Western blot analysis of ALDH2 in Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate using 50 µg per well. Sample was incubated with ALDH2 (Product # PA5-78757) at a dilution of 0.5 µg/mL.
Immunohistochemistry analysis of ALDH2 on paraffin-embedded human kidney cancer tissue. Sample was incubated with ALDH2 polyclonal antibody (Product# PA5-78757).
Immunocytochemistry analysis of ALDH2 using anti-ALDH2 antibody (Product # PA5-78757). ALDH2 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-ALDH2 antibody (Product # PA5-78757)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Supplier Page from Thermo Fisher Scientific for ALDH2 Antibody