Fig 1: Immunofluorescence labeling of the bladders. Representative immunohistochemical images with antibodies against Htr3b (green, a and d), Tubb3 (red, b and e), and merged image (c and f) along nuclei staining using DAPI (blue) on the bladder sections from female mice in the control (a–c) and VCR (d–f) groups. Arrowheads indicate the cluster-forming Tubb3 IR. Representative immunohistochemical images on the bladder sections from male mice in the control (g–i and m–o) and VCR (j–l and p–r) groups with antibodies against Itga1 (green, g and j), Tubb3 (red, h and k), Desmin (green, m and p), Trpa1 (red, n and q) and merged image (i, l, o, and r) along nuclei staining using DAPI (blue). Scale bars = 250 µm. Figures were prepared using Adobe Photoshop CS6.