Western blot analysis of APBB1IP in, Lane 1: human THP-1 whole cell lysates, Lane 2: Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: U937 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with RIAM Polyclonal Antibody (Product # PA5-78787) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for APBB1IP at approximately 110-120KD. The expected band size for APBB1IP is at 73KD.

Immunocytochemistry analysis of RIAM in JURKAT cells. Sample was incubated with RIAM polyclonal antibody (Product # PA5-78787).