Western blot was performed using Anti-PDX1 Polyclonal Antibody (Product # PA5-78024) and a 38 kDa band corresponding to PDX1 was observed across cell lines and tissues tested except Mouse Brain which is reported to be negative. Whole cell extracts (30 µg lysate) of PANC-1 (Lane 1), MIA-PaCa-2 (Lane 2), tissue extracts of Mouse Pancreas (Lane 3) and Mouse Brain (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).