Paxillin Antibody PA5-78142 from Thermo Fisher Scientific

Supplier Images

WB

Western blot was performed using Anti-Paxillin Polyclonal Antibody(Product # PA5-78142) and a 64kDa band corresponding to Paxillin was observed across all tested cell lines and tissues. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), COS-7 (Lane 3), PC-12 (Lane 4), Caco-2 (Lane 5), MCF7 (Lane 6), T-47D (Lane 7), Mouse Lung (Lane 8), Rat Lung (Lane 9), NIH/3T3 (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).An additional band at ~55kDa was observed, which corresponds to the proteolytic cleavage products of Paxillin
ICC/IF

Immunofluorescence analysis of Paxillin was performed using 70 percent confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Paxillin Polyclonal Antibody (Product # PA5-78142) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing focal adhesions localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supplier Page from
Thermo Fisher Scientific for
Paxillin Antibody

Thermo Fisher Scientific's Paxillin Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, and Western Blot. The Paxillin Antibody was generated using Paxillin, and Pxn as the antigen and it reacts with Homo Sapiens, and Human.

WB

ICC/IF

Paxillin Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

Reviews

Paxillin Antibody from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map