For immunofluorescence analysis, HEK-293 cells were stably transfected with human AVPR1A. After 24hr of transfection the cells were fixed and permeabilized for detection of AVPR1A using Anti-AVPR1A Recombinant Rabbit Monoclonal Antibody (Product # 702609, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of the AVPR1A on the transfected cells. The images were captured at 40X magnification.

Sections of Human and Rat Kidney were dewaxed, microwaved in citric acid and incubated with Anti-AVPR1A Recombinant Rabbit Monoclonal Antibody (Product # 702609, 1 µg/mL, 1:500 dilution). Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were further developed in AEC and lightly counterstained with hematoxylin. Panel a & b) shows the specific localization of AVPR1A in Human and Rat Kidney respectively. Panel c) shows the no primary antibody control.