Fig 2: (A) Representative double immunofluorescence stainingphotomicrograph showing neuronal nulei (NeuN; red), myelin basic protein (MBP; green) and cell nuclei (DAPI; blue) in the brain cortex in experimental rats. Quantification ofstaining intensity of (B) NeuN and (C) MBP was performed using ImageJ software. GA gallic acid, AA ascorbic acid.

Fig 3: Myelin basic protein (MBP) and Iba1 immunohistochemistry in spinal cord tissue. Transverse sections of the spinal cord collected from the distal side of the injury (T11–T12). MBP immunofluorescence (red) at 4x magnification (A–E) and 20x (F–J); Iba1 immunofluorescence (green) at 20x magnification (K–O). Arrows and arrowheads show representative cells (resting and active microglia, respectively). Experimental groups: sham (control), spinal cord injury (SCI), GnRH, GH, and GnRH+GH (G+G). Scale bar [(A–E) 50 uM] [(F–O) 20 uM].