Western blot was performed using Anti-PLCB2 Polyclonal Antibody (Product # PA5-78108) and a 134 kDa band corresponding to PLCB2 was observed across cell lines. Whole cell extracts (60 µg lysate) of THP-1 (Lane 1), HEL 92.1.7 (Lane 2), NK-92 (Lane 3), Jurkat (Lane 4), HL-60 (Lane 5), U-937 (Lane 6), HeLa (Lane 7), SK-O-V3 (Lane 8) and A549 (Lane 9) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). THP-1, HEL 92.17, NK-92 are high expressing than Jurkat, HL-60, U-937, HeLa, SK-O-V3 and A549 cell lines for PLCB2.