Purified anti-Tubulin beta 3 (TUBB3) 801213 from BioLegend

Product Specs
ItemPurified anti-Tubulin beta 3 (TUBB3)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number801213
Quantity25 µL
ApplicationsImmunohistochemistry (IHC)-P, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC), SB
Concentration1.00 mg/mL
Conjugate/TagUnconjugated
FormatPhosphate-buffered solution + 0.03% Thimerosal
ReactivityHuman, Mouse, Rat
TargetTubulin beta-3
IsotypeIgG2a, Kappa
CloneTUJ1
HostMouse
ImmunogenThis antibody was raised against microtubules derived from rat brain
NCBI Full Gene Nametubulin beta 3 class III
NCBI Gene AliasesCDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
ClonalityMonoclonal

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Developing Bovine Brain-Derived Extracellular Matrix Hydrogels: a Screen of Decellularization Methods for Their Impact on Biochemical and Mechanical PropertiesACS OmegaOctober 10, 2023Duygu Turan Sorhun, Alican Kuşoğlu, Ece Öztürkserum for 2 h. Primary antibodies anti-Neun (1:300, ab177487, Abcam) and anti-β-Tubulin III (2 μg/mL, #801213, Biolegend) were used for immunolabeling. After overnight incubation at 4 °C in constant rotation, samples

(2) Customization of the translational complex regulates mRNA-specific translation to control CNS regenerationNeuronSeptember 20, 2023Schaeffer J, Vilallongue N, Decourt C, Blot B, El Bakdouri N, Plissonnier E, Excoffier B, Paccard A, Diaz JJ, Humbert S, et al.HPA058396) 1:100; mouse anti-puromycin (Sigma-Aldrich, MABE343) 1:250; mouse anti-Tubulin β III (Biolegend, 801213) 1:500; rabbit anti-SCG10 (Novus Biologicals, NBP1-49461) 1:1000. After several washes in PBS, sections

(3) Induction of MYCN-amplified neuroblastoma differentiation through NMYC suppression using PPAR-γ antagonistJournal of Clinical Biochemistry and NutritionJune 28, 2023Yukako Nakao-Ise, Takumi Narita, Shingo Miyamoto, Motoki Watanabe, Takuji Tanaka, Yoshihiro Sowa, Yosuke Iizumi, Mitsuharu Masuda, Gen Fujii, Yasuko Hirai, et al.they were then incubated for 1 h and stained with a primary antibody against tubulin beta 3 (TUBB3) (801213; Biolegend, San Diego, CA) and MAP2 (ab32454; Abcam) in PBS solution containing 1% bovine serum albumin (BSA

(4) Injury-induced activation of the endocannabinoid system promotes axon regenerationiScienceJune 16, 2023Martinez-Torres S, Mesquida-Veny F, Del Rio JA, Hervera A.anti-Stathmin-2,Novus,"Cat# NBP1-49461, RRID:AB_10011569" Mouse monoclonal anti-Tubulin β 3,Biolegend,"Clone: Tuj-1, Cat# 801213, RRID:AB_2728521" Rabbit monoclonal anti-pAkt (Ser473),Cell Signaling Technology,"Clone, D9E, Cat#

(5) Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after strokeiScienceJanuary 20, 2023Jin S, Chen X, Zheng H, Cai W, Lin X, Kong X, Ni Y, Ye J, Li X, Shen L, et al.RRID:AB_2862549" Mouse anti-HA.11 Epitope Tag,Biolegend,"Cat# 901516, RRID:AB_2820200" Mouse anti-TUJ1,BioLegend,"Cat# 801213, RRID:AB_2728521" Mouse anti-p53,Cell Signaling,"Cat# 2524, RRID:AB_331743" Mouse anti-mTOR,Cell Signaling,"Cat#

(6) Highly sensitive and non-disruptive detection of residual undifferentiated cells by measuring miRNAs in culture supernatantScientific ReportsDecember 1, 2022Kanako Masumoto, Yuki Aihara, Mao Miyagawa Kuroishi, Natsuki Maeda, Yumiko Sakai, Yuma Oka, Yusuke Takahashi, Kenta Oda, Masatoshi Yanagidadifferentiation into NPCs, the cells were fixed using 4% paraformaldehyde (PFA) and stained with TUJ1 antibody (801213, BioLegend) and DAPI. Preparation of residual undifferentiated cell model iPSCs (7 × 104) were fixed

(7) Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammalseLifeNovember 29, 2022Iyer AA, Hosamani I, Nguyen JD, Cai T, Singh S, McGovern MM, Beyer L, Zhang H, Jen HI, Yousaf R, et al.25–6790,AB_10015251,1:300 Myosin VIIa,Mouse polyclonal,DSHB 138–1,AB_2282417,1:200 TUJ1,Mouse polyclonal,BioLegend 801213,AB_2728521,1:1000 VGLUT3,Rabbit polyclonal,Synaptic systems 135203,AB_887886,1:300 PRESTIN,Rabbit

(8) Prognostic Analysis of Human Pluripotent Stem Cells Based on Their Morphological Portrait and Expression of Pluripotent MarkersInternational journal of molecular sciencesOctober 26, 2022Krasnova OA, Gursky VV, Chabina AS, Kulakova KA, Alekseenko LL, Panova AV, Kiselev SL, Neganova IE.differentiated EBs were stained for germ layer markers with antibodies anti-TUJ1 (BioLegend, San Diego, CA, USA; 801213), anti-AFP (GeneTex, Irvine, CA, USA; GTX131311), and anti-alpha-SMA (Sigma Aldrich, St. Louis, MO,

(9) Modeling Human Cerebellar Development In Vitro in 2D StructureJournal of Visualized ExperimentsOctober 3, 2022Deniz A. Madencioglu, Karina A. Kruth, Thomas H. Wassink, Vincent A. Magnotta, John A. Wemmie, Aislinn J. WilliamsSystems,MAB7617, "Human anti-PTF1a, rabbit",Novus Biologicals,NBP2-98726, "Human anti-TUBB3, mouse",Biolegend,801213, IMDM,Gibco,12440053, Insulin,Gibco,12585, Laminin Mouse Protein,Gibco,23017015, Matrigel Matrix,Corning,354234,Basement

(10) Severe congenital myasthenic syndromes caused by agrin mutations affecting secretion by motoneuronsActa neuropathologicaOctober 1, 2022Jacquier A, Risson V, Simonet T, Roussange F, Lacoste N, Ribault S, Carras J, Theuriet J, Girard E, Grosjean I, et al.polyclonal anti-GRP78/BIP (#11587–1-AP; Proteintech; for WB 1:3000); mouse monoclonal anti-Tuj1 (# 801213; BioLegend; for IF 1:1000); goat polyclonal anti-Islet1 (#GT15051; Neuromics, for IF 1:200); mouse monoclonal

(11) Archetype tasks link intratumoral heterogeneity to plasticity and cancer hallmarks in small cell lung cancerCell SystemsSeptember 1, 2022Sarah M. Groves, Geena V. Ildefonso, Caitlin O. McAtee, Patricia M.M. Ozawa, Abbie S. Ireland, Philip E. Stauffer, Perry T. Wasdin, Xiaomeng Huang, Yi Qiao, Jing Shan Lim, et al.saponin, with PBS washes in between. Cells were incubated with anti-Tubulin beta 3 (TUBB3 Clone TUJ1, Cat# 801213, Biolegend) diluted 1:500 in Blocking solution for 1 hour at room temperature. Cells were washed with

(12) Silencing of circular RNA‑ZYG11B exerts a neuroprotective effect against retinal neurodegenerationInternational Journal of Molecular MedicineJune 21, 2022Cong Ma, Mu-Di Yao, Xiao-Yan Han, Ze-Hui Shi, Biao Yan, Jian-Ling Duno. ab7260; Abcam), NeuN (1:300; cat. no. ab128886; Abcam) or anti-tubulin β3 (TUJ1; 1:300; cat. no. 801213; Biolegend, Inc.) overnight at 4°C. The slides were then washed with PBS containing 0.1% Tween 20 and

(13) Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoidsSTAR protocolsJune 17, 2022Eriksen AZ, Møller R, Makovoz B, tenOever BR, Blenkinsop TA.ab92516Lot GR3185160-1; RRID: AB_10562796 Purified anti-Tubulin β 3 (TUBB3) antibody,BioLegend,Cat# 801213; RRID: AB_2728521 Sheep anti Human chx 10 (Visual system homeobox 2) (CT),Exalpha,Cat# X1179P;Lot 13703;

(14) TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13ANatureMarch 1, 2022Brown AL, Wilkins OG, Keuss MJ, Hill SE, Zanovello M, Lee WC, Bampton A, Lee FCY, Masino L, Qi YA, et al.

(15) Sarm1 activation produces cADPR to increase intra-axonal Ca++ and promote axon degeneration in PIPNJournal of Cell BiologyFebruary 7, 2022Yihang Li et al.0.1% Triton X-100 for 1 h at room temperature and incubated with mouse anti-Tuj1 (1:500; BioLegend; 801213) overnight at 4°C. Cultures were then washed twice with PBS and incubated with goat anti-mouse Alexa

(16) Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineageDevelopmentJanuary 15, 2022Sophie Colombo, Valérie Petit, Roselyne Y. Wagner, Delphine Champeval, Ichiro Yajima, Franck Gesbert, Zackie Aktary, Irwin Davidson, Véronique Delmas, Lionel Larueβ-galactosidase (Abcam ab9361, 1/400), mouse monoclonal antibody against tubulin β3 (clone TUJ1, BioLegend 801213, 1/200), rabbit monoclonal antibody against gp100/Pmel (Abcam ab137078, 1/300) and rabbit polyclonal

(17) Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene EditingCellsJanuary 13, 2022Nouri P, Zimmer A, Brüggemann S, Friedrich R, Kühn R, Prakash N.1 SOX2,Rabbit,1:250,ab97959,abcam TRA-1-60,Mouse,1:500,ab16288,abcam TH,Mouse,1:1000,MAB318,Merck Millipore TH,Rabbit,1:1000,AB152,Merck Millipore TUBB3,Mouse,1:1000,801213,BioLegend Primary antibodies for ICC and WB. 1 For WB. 2 Developmental Studies Hybridoma Bank, University

(18) Myelinating Schwann cells and Netrin-1 control intra-nervous vascularization of the developing mouse sciatic nerveeLifeJanuary 12, 2022Taïb S, Lamandé N, Martin S, Coulpier F, Topilko P, Brunet I.IgG2a),R&D,"Cat# BAM1195, RRID:AB_356859",(1:500) Antibody,Tuj-1(mouse monoclonal IgG2a),Biolegend,"Cat# 801213, RRID:AB_2728521",(1:400) Antibody,SMA-cy3(mouse monoclonal),Sigma,"Cat# C6198, RRID:AB_476856",(1:500) Antibody,Neurofilament

(19) The histone methyltransferase G9a mediates stress-regulated alcohol drinkingAddiction biologyJanuary 1, 2022Anderson EM, Lopez MF, Kastner A, Mulholland PJ, Becker HC, Cowan CW.

(20) Nuclear IMPDH Filaments in Human GliomasJournal of neuropathology and experimental neurologyOctober 26, 2021Ahangari N, Munoz DG, Coulombe J, Gray DA, Engle EC, Cheng L, Woulfe J."Millipore Sigma, Burlington, MA, USA",T8660 TUBB3,TUJ1,mouse monoclonal,1:200,"Biolegend, San Diego, CA, USA",801213 TUBB3,TU20,mouse monoclonal,1:500,"Millipore Sigma, Burlington, MA, USA",MAB1637 TUBB3,N/A,rabbit

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(1)

11/8/2019Excellent Antibody for Tubulin b3/Tuj1 from BioLegend Elena SeranovaWould recommend for characterizing neuronal cultures. Can be combined with other assays and fluorescent labels.
(1)

6/6/2019Very Good Tubulin Beta 3 Antibody from BioLegend Elva ZhangI recommend that this antibody to be commonly used in immunofluorescent staining of neurons, no matter in CNS or in PNS.

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Purified anti-Tubulin beta 3 (TUBB3) from BioLegend

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BioLegend

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Purified anti-Tubulin beta 3 (TUBB3) from BioLegend

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