ASAP1 antibody orb420376 from Biorbyt

Product Specs
ItemASAP1 antibody
CompanyBiorbyt
Price $292.00Supplier Page
Catalog Numberorb420376
Quantity25 µl
ApplicationsELISA, WB
Concentration1.04 mg/ml
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityThis affinity purified antibody is directed against human ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity to human 100%, bovine 93%, and rat and mouse 87% homology for the immunogen sequence. Cross-reactivity from other sources has not been determined
ReactivityHuman
TargetASAP1
IsotypeIgG
HostRabbit
ImmunogenAnti-ASAP1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-terminus region of human ASAP1 protein
NCBI Full Gene NameArfGAP with SH3 domain, ankyrin repeat and PH domain 1
NCBI Gene AliasesPAP, CENTB4, AMAP1, PAG2, ZG14P, DDEF1
ClonalityPolyclonal
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing

Specifications/Features

Application Notes: Anti-ASAP1 Antibody has been tested for use in ELISA and Western Blot. Expect a band approximately ~125kDa using positive control lysates over-expressing ASAP1, or other specific lysates or tissues. Although not tested, this antibody could be useful in Immunofluorescence or Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Application Dilutions: ELISA: 1:50,000, WB: 1µg/mL

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(1) Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3'UTR bindingScientific reportsJuly 16, 2020Garone MG, Alfano V, Salvatori B, Braccia C, Peruzzi G, Colantoni A, Bozzoni I, Armirotti A, Rosa A.Western blot. Western blot analysis was carried out using anti-VCP (5) (sc-57492; Santa Cruz Biotechnology), anti-ASAP1 (orb420376; Biorbyt), anti-TUBB3 (T2200; Sigma-Aldrich), anti-GAPDH (MAB-10578; Immunological sciences) primary

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ASAP1 antibody from Biorbyt

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Specifications/Features

Biorbyt

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ASAP1 antibody from Biorbyt

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