MyBioSource.com's Rabbit anti NFkB p65 Polyclonal Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blot. The Rabbit anti NFkB p65 Polyclonal Antibody was generated using RELA as the antigen and it reacts with Human, and Rat.
Description
NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls the transcription of DNA. NF-kB is important in regulating cellular responses because it belongs to the category of "rapid-acting" primary transcription factors. NF kappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit. In unstimulated cells, the NF-kB dimers are sequestered in the cytoplasm by a family of inhibitors, called IkBs (Inhibitor of kB). Activation of the NF-kB is initiated by the signal-induced degradation of IkB proteins. Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor. NF-kB complex enters the nucleus where it can 'turn on' the expression of specific genes that have DNA-binding sites for NF-kB nearby. The activation of these genes by NF-kB then leads to the given physiological response, for example, an inflammatory or immune response, a cell survival response, or cellular proliferation