Fig 2: Ki67 Immunohistochemistry in the kidney. (A) Ki67 Immunohistochemistry Images. Representative 20× images of kidney sections stained for the proliferative marker Ki67 (brown DAB signal). Untreated animals exhibit markedly increased cell proliferation, particularly in the tubular epithelial cells, which is significantly reduced in CG-treated animals. Uninjured controls demonstrate negligible proliferation. (B) Quantification of Ki67 Positive Area. Bar graph representing the percent area of Ki67-positive signal, quantified separately for the renal cortex and medulla using color deconvolution (ImageJ FIJI). CG significantly reduced I/R-induced proliferation in both zones compared to the untreated. Images obtained on Leica DM500 LED microscope. Scale bar represents 50 microns (μm). * p < 0.05; ** p < 0.01.