MyBioSource.com's Anti-PRMT5 Rabbit Monoclonal Antibody is a Rabbit monoclonal antibody. The Anti-PRMT5 Rabbit Monoclonal Antibody was generated using PRMT5 as the antigen. It reacts with Human, Mouse, and Rat. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot.
Description
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA (PubMed: 10531356, PubMed: 11152681, PubMed: 11747828, PubMed: 12411503, PubMed: 15737618, PubMed: 17709427, PubMed: 20159986, PubMed: 20810653, PubMed: 21258366, PubMed: 21917714, PubMed: 22269951). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles (PubMed: 12411503, PubMed: 11747828, PubMed: 17709427). Methylates SUPT5H and may regulate its transcriptional elongation properties (PubMed: 12718890). Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity). Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation (PubMed: 21917714, PubMed: 21258366). Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity (PubMed: 21917714). Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9 (PubMed: 22269951). Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (PubMed: 20810653). Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter (By similarity). Methylates GM130/GOLGA2, regulating Golgi ribbon formation (PubMed: 20421892). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed: 25284789). Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed: 26700805)