Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.