Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a RLIM monoclonal antibody (Product # MA5-27516) at a dilution of 1:500.

Western blot analysis of RLIM in HepG2, HeLa, A549 cells using 35 µg per lane. Samples were probed with RLIM (Product # MA5-27516) monoclonal antibody at a dilution of 1:500.