Immunohistochemistry was performed on paraffin-embedded human gastric tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a RPL7A monoclonal antibody (Product # MA5-27534) at a dilution of 1:500.

Western blot analysis of RPL7A in A431, Raji, HeLa, MCF7, 293T, Raw264.7, NIH/3T3 cells using 35 µg per lane. Samples were probed with RPL7A (Product # MA5-27534) monoclonal antibody at a dilution of 1:500.