Fig 2: The associations between six genes and survival rates are shown in cervical cancer patientsAnalysis of the correlation between PLS1, TM7SF2, PYCR1, SLC19A1, TTYH3, and NIPSNAP1 expression and survival rates in patients with HPV-negative cervical cancer (22 samples), patients with HPV-positive cervical cancer (281 samples), and all patients with cervical cancer (304 samples). All samples information was obtained from the TCGA.

Fig 3: The protein levels of eleven upregulated genes in HPV-negative cervical cancer tissuesThe protein expression level was determined by calculating immunohistochemical staining score. Immunohistochemical staining scores for 11 proteins, PRAME, HMGA2, SBK1, MEX3A, TM7SF2, SLC19A1, ETV4, PYCR1, TTYH3, PLS1, and NIPSNAP1, in HPV-negative cervical cancer tissues, HPV-positive cervical cancer tissues, and normal cervical tissues are shown. **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data are presented as the means ± SD from triplicate experiments.

Fig 4: Representative image of immunohistochemical staining for several genesThe images of ETV4, PYCR1, TTYH3, PLS1, and NIPSNAP1 were presented in HPV-negative cervical cancer tissues, HPV-positive cervical cancer tissues, and normal cervical tissues.

Fig 5: The mRNA expression of seventeen upregulated genes in HPV-negative cervical cancer cellsC33A is an HPV-negative cervical cancer cell line. SiHa, Caski, MS751, and HeLa cells are HPV-positive cervical cancer cell lines. Ect1 is a normal cervical squamous cell line. (A and B) The mRNA expression of 17 upregulated genes, including PRAME, HMGA2, SBK1, ETV4, MEX3A, PLS1, TM7SF2, NIPSNAP1, PYCR1, SLC19A1, TTYH3 (A), MT1G, SLC7A5, RHPN1, CHD7, HSPH1, and SLC25A13 (B) was detected in different cells. *p < 0.05, **p < 0.01, ***p < 0.001, and ns, not significant. Data are presented as the means ± SD from triplicate experiments.