Mouse IL-15R alpha Biotinylated Antibody BAF551 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse IL-15R alpha Biotinylated Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberBAF551
ApplicationsFlow Cytometry,Western Blot,Immunocytochemistry,Functional Assay
HostGoat
Conjugate/TagBiotin
IsotypeIgG
ReactivityMouse
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
TargetIL-15R alpha
Quantity50 ug
NCBI Full Gene Nameinterleukin 15 receptor, alpha chain
ClonalityPolyclonal
ConcentrationLYOPH
PurityAntigen Affinity-purified
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
Antibody TypePrimary Antibodies
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha, Gly33-Lys205, Accession # Q60819
NCBI Gene AliasesIL-15RA

Specifications/Features

Available conjugates: Biotin

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) NK cells mediate clearance of CD8(+) T cell-resistant tumors in response to STING agonistsScience immunologyMarch 20, 2020Nicolai CJ, Wolf N, Chang IC, Kirn G, Marcus A, Ndubaku CO, McWhirter SM, Raulet DH.(MAR1–5A3); BD Biosciences: anti-H-2Kb (AF6–88.5) and anti-granzyme B (GB11); R&D Systems: anti-IL-15Rα (BAF551). Ex vivo cytotoxicity assay Cytotoxicity by splenocytes was assessed with a standard 4-hour 51Cr-release

(2) NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial ImmunityJournal of immunology (Baltimore, Md. : 1950)March 15, 2019Simonović N, Witalisz-Siepracka A, Meissl K, Lassnig C, Reichart U, Kolbe T, Farlik M, Bock C, Sexl V, Müller M, et al.

(3) Murine Th17 cells utilize IL-2 receptor gamma chain cytokines but are resistant to cytokine withdrawal-induced apoptosisCancer immunology, immunotherapy : CIIJune 1, 2017Neitzke DJ, Bowers JS, Andrijauskaite K, O'Connell NS, Garrett-Mayer E, Wrangle J, Li Z, Paulos CM, Cole DJ, Rubinstein MP.in PMC 2018 June 01. Neitzke et al. Page 4 Author Manuscript biotinylated polyclonal IL15Rα (BAF551, R&D Systems), which was detected by secondary stain with streptavidin (eBioscience). Cytokine production

(4) IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopeniaCell & bioscienceJanuary 1, 2016Xu A, Bhanumathy KK, Wu J, Ye Z, Freywald A, Leary SC, Li R, Xiang J.

(5) IL-15 receptor α signaling constrains the development of IL-17-producing γδ T cellsProceedings of the National Academy of Sciences of the United States of AmericaAugust 4, 2015Colpitts SL, Puddington L, Lefrançois L.

(6) Autophagy is a critical regulator of memory CD8(+) T cell formationeLifeNovember 11, 2014Puleston DJ, Zhang H, Powell TJ, Lipina E, Sims S, Panse I, Watson AS, Cerundolo V, Townsend AR, Klenerman P, et al.(B8.2C12) PE/APC; Bcl-2 (BCL/10C4) PE; all from Biolegend (San Diego, CA, USA). Glut1 (q) PerCP; IL-15Rα (BAF551) biotinylated; both from R&D Systems (Minneapolis, USA). For surface staining, cells were suspended

(7) Protein energy malnutrition impairs homeostatic proliferation of memory CD8 T cellsJournal of immunology (Baltimore, Md. : 1950)January 1, 2012Iyer SS, Chatraw JH, Tan WG, Wherry EJ, Becker TC, Ahmed R, Kapasi ZF.Ki-67-FITC (B56) (all from BD Biosciences), CD127-PE-Cy7 (A7R34) (eBioscience, San Diego, CA), IL-15Ra-FITC (BAF551) (R&D Systems, Minneapolis, MN), CD44-Pacific blue (IM7) (Biolegend, San Diego, CA). Intracellular staining

(8) Different NK cell developmental events require different levels of IL-15 trans-presentationJournal of immunology (Baltimore, Md. : 1950)August 1, 2011Lee GA, Liou YH, Wang SW, Ko KL, Jiang ST, Liao NS.

(9) Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15RαBloodApril 14, 2011Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y.

(10) No Requirement ofTransPresentations of IL-15 for Human CD8 T Cell ProliferationThe Journal of ImmunologyNovember 15, 2010Naruhisa Ota, Mitsuyo Takase, Hidemi Uchiyama, Shaun K. Olsen, Osami Kanagawa(Tokyo University, Tokyo, Japan) and cultured in DMEM containing 10% calf serum. Anti-mouse IL-15Ra (BAF551) and anti-mouse CD122 (Rb, 1321221) biotin-conjugated Abs were purchased from R&D Systems (Minneapolis,

(11) Qualitatively different memory CD8+ T cells are generated after lymphocytic choriomeningitis virus and influenza virus infectionsJournal of immunology (Baltimore, Md. : 1950)August 15, 2010Mueller SN, Langley WA, Li G, García-Sastre A, Webby RJ, Ahmed R.(JES6-5H4) (all from BD Biosciences), CD127-PE (A7R34) (eBioscience, San Diego, CA), biotinylated IL15Ra (BAF551) (R&D Systems, Minneapolis, MN), or anti-human granzyme B-PE (Caltag Laboratories, Burlingame, CA).

(12) IL-18, but not IL-15, contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum in vivoEuropean journal of immunologyJune 1, 2010Haeberlein S, Sebald H, Bogdan C, Schleicher U.(HL3), anti-I-A/I-E (M5/114.15.2), anti-F4/80 (CI:A3-1, AbD Serotec, Düsseldorf, Germany), anti-IL-15Rα (BAF551, R&D Systems, Wiesbaden, Germany), anti-NK1.1 (PK136), anti-NKp46 (29A1.4; eBioscience, Hatfield, UK),

(13) Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cellsProceedings of the National Academy of Sciences of the United States of AmericaMay 25, 2010Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, et al.(HM40-3), CD80 (16-10A1), CD86 (GL1), MHC class II (M5/114, 15.2), Ly49H (3D10) (eBioscience), and IL15Ra (BAF551) (R&D Systems). Statistical Analysis. The statistical signiﬁcance of differences was determined by a

(14) IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activityJournal of immunology (Baltimore, Md. : 1950)June 15, 2009Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, et al.

(15) Interleukin-15 combined with an anti-CD40 antibody provides enhanced therapeutic efficacy for murine models of colon cancerProceedings of the National Academy of Sciences of the United States of AmericaMay 5, 2009Zhang M, Yao Z, Dubois S, Ju W, Müller JR, Waldmann TA.

(16) Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in transJournal of immunology (Baltimore, Md. : 1950)December 15, 2008Rowley J, Monie A, Hung CF, Wu TC.have been previously reported (28 –30). Biotinylated IL-15 (catalog no. BAF447) and IL-15RA (catalog no. BAF551) Abs were purchased from R&D Systems. Murine NK cell and CD8 T cell-negative selection kits were purchased

(17) IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune responseBloodNovember 1, 2008Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW.

(18) IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell developmentJournal of immunology (Baltimore, Md. : 1950)September 1, 2008Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA.

(19) A synaptic basis for paracrine interleukin-2 signaling during homotypic T cell interactionImmunityAugust 15, 2008Sabatos CA, Doh J, Chakravarti S, Friedman RS, Pandurangi PG, Tooley AJ, Krummel MF.

(20) IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentationThe Journal of experimental medicineMay 12, 2008Mortier E, Woo T, Advincula R, Gozalo S, Ma A.(PeproTech). IL-15Rα expression on cell surfaces was detected by using biotinylated anti–IL-15Rα antibody BAF551 (R&D Systems) as described previously (15). IFN-γ and granzyme B production by NK cells was determined

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Mouse IL-15R alpha Biotinylated Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Mouse IL-15R alpha Biotinylated Antibody from R&D Systems, a Bio-Techne Brand

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