PDGF R beta Antibody MAB1263 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPDGF R beta Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $449.00Supplier Page View Company Product Page
Catalog NumberMAB1263
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunocytochemistry
ReactivityHuman,Mouse
HostMouse
Conjugate/TagUnconjugated
ClonePR7212
Quantity250 ug (also 25 ug)
TargetPDGF R beta
IsotypeIgG1
ClonalityMonoclonal
PurityProtein A or G purified from ascites
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening
NCBI Full Gene Nameplatelet derived growth factor receptor beta
NCBI Gene AliasesCD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT
ImmunogenHuman skin fibroblast membrane extracts
FormatSupplied as a 0.2 µm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human PDGF Rß in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor ß-subunit complexed with 125I-PDGF-BB (3). It does not recognize the PDGF receptor alpha -subunit. Detects the PDGF receptor ß-subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.

Sizes Available: 250 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study PDGFRβ in human fibroblasts?

Yes, the PDGF R beta Antibody has been shown to effectively detect human PDGF Rβ in various applications, including immunocytochemistry and Western blotting, as evidenced by its use in studies involving human dermal fibroblasts and cancer-associated fibroblasts.

Is this antibody suitable for use in flow cytometry?

The PDGF R beta Antibody has been utilized in flow cytometry, as indicated by its application in studies that involve sorting and analyzing fibroblast populations, such as in the characterization of cancer-associated fibroblasts.

What species does this antibody react with?

This antibody specifically detects the PDGF receptor β-subunit in human and primate species, including monkeys and baboons. However, it does not recognize the PDGF receptor alpha-subunit or receptors from rat or mouse species.

Are there any specific experimental conditions or concentrations recommended for using this antibody?

In various studies, the PDGF R beta Antibody has been used at dilutions ranging from 1:200 to 1:1000, depending on the application, such as immunohistochemistry or Western blotting, which suggests that optimization may be necessary based on specific experimental conditions.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) AMPK-p38 axis converts human pluripotent stem cells to naive stateiScienceMay 15, 2026Zhennan Yang, Yajing Liu, Huaigeng Xu, Junko Yamane, Akitsu Hotta, Wataru Fujibuchi, Jun K. YamashitaAB_1857931 Rabbit anti-Thy1,Gene Tex,Cat# GTX130072; RRID: AB_ Mouse anti-PDGFR-β (clone PR7212),R&D Systems,Cat# MAB1263; RRIS: AB_2886168 Goat anti-SOX17,R&D Systems,Cat# AF1924; RRID: AB_355060 Mouse anti-CXCR4 Antibody

(2) PHLDA1 is a shared diagnostic and key mediator of inflammatory fibrosis in heart and kidneyFrontiers in ImmunologyFebruary 5, 2026Lei Hua, Liangru Shen, Yongshou Tao, Chentong Wang, Xiaohang Shaofrom another donor (73 years old, male, eGFR: 42 ml/min/1.73 m2) was obtained with PDGFRβ antibody (MAB1263, R&D Systems, dilution: 1:100) and separated by magnetic microbeads (130-047-102, Miltenyi Biotec).

(3) Immunohistochemical and Ultrastructural Characterization of Telocytes in Normal and Diabetic Human KidneysBiomoleculesAugust 8, 2024Valente S, Villacampa Lahoz M, Vasuri F, Pasquinelli G.

(4) Gliovascular transcriptional perturbations in Alzheimer's disease reveal molecular mechanisms of blood brain barrier dysfunctionNature communicationsJune 20, 2024İş Ö, Wang X, Reddy JS, Min Y, Yilmaz E, Bhattarai P, Patel T, Bergman J, Quicksall Z, Heckman MG, et al.

(5) Unraveling the role of natriuretic peptide clearance receptor (NPR3) in glomerular diseasesScientific ReportsMay 24, 2024Dina Dabaghie, Emmanuelle Charrin, Pernilla Tonelius, Birgitta Rosengren, Gizem Korkut, Anna B. Granqvist, Mark Lal, Jaakko Patrakka(ab97389, abcam, 1:1000), Synaptopodin (61094; Progen, 1:700), CD31 (303105; Biolegend, 1:500), PDGFRβ (MAB1263; R&D System, 1:1000), Nephrin (BP5030; Origene, 1:200), Wilms-Tumor1 (WT1) (ab89901; abcam, 1:100),

(6) Evidence of steady-state fibroblast subtypes in the normal human breast as cells-of-origin for perturbed-state fibroblasts in breast cancerBreast Cancer ResearchJanuary 16, 2024Mikkel Morsing Bagger, Jonas Sjölund, Jiyoung Kim, Katharina Theresa Kohler, René Villadsen, Abbas Jafari, Moustapha Kassem, Kristian Pietras, Lone Rønnov-Jessen, Ole William Petersen,AbD Serotec/MCA1557A488,,,1:25, CD105,SN6,Abcam/Ab11414,1:100,,,M CD26,202–36,Abcam/Ab3154,1:50,,,M CD140b,PR7212,R&D Systems/MAB1263,1:200,,,F1 Tenascin,BC-24,"Sigma, T2551",1:5000,,,M Vimentin,SP20,ThermoFisher Scientific/RM-9120,1:200,,,FFPE α-sm

(7) Generation of vascularized human cardiac organoids for 3D in vitro modelingSTAR protocolsSeptember 15, 2023Voges HK, Mills RJ, Porrello ER, Hudson JE.1:1000",Sigma,RRID: AB_476766 "Mouse anti-PDGF Rβ Monoclonal Antibody, use 1:250",R&D Systems,Cat# MAB1263 "Mouse IgG2B anti-VE-cadherin antibody, use 1:100",R&D Systems,RRID: AB_2260374 "Goat anti-Mouse IgG2a

(8) Proteomics profiling identifies extracellular vesicles' cargo associated with tumour cell induced platelet aggregationBMC cancerSeptember 29, 2022McNamee N, de la Fuente LR, Santos-Martinez MJ, O'Driscoll L.

(9) Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal-epidermal junctionScientific reportsJanuary 17, 2022Iriyama S, Ogura Y, Nishikawa S, Hosoi J, Amano S.keratin-14 (20R-CP002, guinea pig pAb, Fitzgerald Industries International, Acton, MA) and PDGFRbeta (MAB1263, mouse mAb, R&D Systems, Minneapolis, MN). Primary antibodies were detected using Alexa488- or Alexa594-conjugated

(10) Single-cell RNA sequencing reveals the mesangial identity and species diversity of glomerular cell transcriptomesNature communicationsApril 9, 2021He B, Chen P, Zambrano S, Dabaghie D, Hu Y, Möller-Hackbarth K, Unnersjö-Jess D, Korkut GG, Charrin E, Jeansson M, et al.instructions. Then, cells were fixed with 4% PFA-PBS and stained with anti-mouse PDGFRB primary antibody (MAB1263, 1:1000, R&D Systems), followed by an Alexa fluor 488 anti-mouse secondary antibody (A-11001, 1:3000,

(11) Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancerOncology reportsDecember 1, 2020Khan T, Seddon AM, Dalgleish AG, Khelwatty S, Ioannou N, Mudan S, Modjtahedi H.(MAB3481), anti-HER4 (MAB11311), ALK7 (MAB77491), HGF R/c-MET (MAB3582), PDGFRα (MAB1264), PDGFRβ (MAB1263) and IGF-IR (MAB391) were purchased from R&D Systems (Europe Ltd. UK) and Insight Biotechnology (Middlesex,

(12) Comparing the Effect of TGF-β Receptor Inhibition on Human Perivascular Mesenchymal Stromal Cells Derived from Endometrium, Bone Marrow and Adipose TissuesJournal of personalized medicineDecember 1, 2020Gurung S, Ulrich D, Sturm M, Rosamilia A, Werkmeister JA, Gargett CE.

(13) Fibroblasts direct differentiation of human breast epithelial progenitorsBreast cancer research : BCRSeptember 29, 2020Morsing M, Kim J, Villadsen R, Goldhammer N, Jafari A, Kassem M, Petersen OW, Rønnov-Jessen L.no,Peroxidase,Fluorescence,FACS,Fixation α-SMA,1A4,Sigma/A2547,,1:5000,,F1 CD105,SN6,Abcam/Ab11414,1:200,,,F2/M CD26,202-36,Abcam/Ab3154,1:50,,,F2/M CD140b,PR7212,R&D Systems/MAB1263,1:1000–1:2000,,,F1 CD248,EPR17081,Abcam/ab204914,1:1000–1:2500,,,F1 K17,E3,DAKO/M7046,,1:50,,F1/F2/M K14,LL002,Monosan/MONX10687,,1:25–1:50,,F1/F2/M K19,Ba16,GenWay/GWB22664E,1:200,,,F2/M K19,Ba16,Abcam/ab20210,1:200,1:50,,F2/M K19,A53-B/A2,Abcam/ab7754,,1:100,,F/M CD271,ME20.4,BioLegend/345102,,1:25,,F1 CD271-APC,ME20.4,Cedarlane/CL10013APC,,,1:50, CD326–488,9C4,BioLegend/324210,,,1:50, CD326,9C4,BioLegend/324202,,1:25,,F1 MUC1,115D8,Biogenesis/1510-5025,,1:10–1:20,1:50,F2 Vimentin,SP20,Thermo Fisher Scientific

(14) A novel sensitive assay for detection of a biomarker of pericyte injury in cerebrospinal fluidAlzheimer's & dementia : the journal of the Alzheimer's AssociationJune 1, 2020Sweeney MD, Sagare AP, Pachicano M, Harrington MG, Joe E, Chui HC, Schneider LS, Montagne A, Ringman JM, Fagan AM, et al.human PDGFRβ monoclonal mouse IgG against PDGFRβ protein in human skin fibroblast extracts (Catalog no. MAB1263, R&D Systems); human PDGFRβ monoclonal mouse IgG raised against amino acids 33–531 (Catalog no. MAB385,

(15) Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signalingCell death & diseaseMay 4, 2020Wei ST, Huang YC, Hsieh ML, Lin YJ, Shyu WC, Chen HC, Hsieh CH.CXCR7 (GTX100027, GeneTex Inc., 1:500 dilution), PDGFRα (MAB322, R&D Systems, 1:2000 dilution), PDGFRβ (MAB1263, R&D Systems, 1:1000 dilution), VEGFR1 (AF321, R&D Systems, 1:1000 dilution), VEGFR2 (AF357, R&D Systems,

(16) GPRC5b Modulates Inflammatory Response in Glomerular Diseases via NF-κB PathwayJournal of the American Society of Nephrology : JASNSeptember 1, 2019Zambrano S, Möller-Hackbarth K, Li X, Rodriguez PQ, Charrin E, Schwarz A, Nyström J, Wernerson AÖ, Lal M, Patrakka J.HPA015247, 1:500; Atlas Antibodies), CD31 (catalog number 303106, 1:2000; Biolegend), PDGFRb (catalog number MAB1263, 1:1000; R&D System), CD45 (catalog number ab64100, 1:250; Abcam), mouse nephrin (catalog number BP5030,

(17) Spatial and temporal characterization of endometrial mesenchymal stem-like cells activity during the menstrual cycleExperimental cell researchJanuary 1, 2017Xu S, Chan RWS, Ng EHY, Yeung WSB.

(18) A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migrationNature communicationsJuly 13, 2015Cao X, Kaneko T, Li JS, Liu AD, Voss C, Li SS.Fermentas Life Sciences. Mouse anti-human PDGFR-α (MAB322, R.D. Systems) and Mouse anti-human PDGFR-β (MAB1263, R.D. Systems) were gifts from Zia Khan (Western University, Canada). The following antibodies were

(19) The tyrosine phosphatase SHP-2 controls urokinase-dependent signaling and functions in human vascular smooth muscle cellsExperimental cell researchApril 1, 2009Kiyan J, Haller H, Dumler I.

(20) PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokinesInvestigative ophthalmology & visual scienceDecember 1, 2007Li R, Maminishkis A, Wang FE, Miller SS.

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Human PDGF R beta Antibody