BID Antibody AF860 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemBID Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF860
ApplicationsImmunoprecipitation,Western Blot,Simple Western,Immunocytochemistry
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Plant - Arabidopsis thaliana
HostGoat
TargetBID
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse BID, Met1-Asp195, Accession # AAC71064
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameBH3 interacting domain death agonist
NCBI Gene AliasesFP497

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl-terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Ganglioside GA2-mediated caspase-11 activation drives macrophage pyroptosis aggravating intimal hyperplasia after arterial injuryInternational journal of biological sciencesJanuary 1, 2025Shi Y, He T, Liu H, Li X, Li Z, Wen Q, Dai Z, Sun X, Tan Q, Yang W, et al.(1:1000, 9508T, CST), caspase-3 (1:1000, 9662S, CST), cytochrome C (1:1000, 11940S, CST), BID (1:1000, AF860, R&D Systems), BIM (1:500, sc-374358, Santa Cruz), PUMA (1:500, sc-374223, Santa Cruz), COX IV (1:5000,

(2) Osteosarcoma cells depend on MCL-1 for survival, and osteosarcoma metastases respond to MCL-1 antagonism plus regorafenib in vivoBMC CancerNovember 4, 2024Yanhao Ji, Michael A. Harris, Lucas M. Newton, Tiffany J. Harris, W. Douglas Fairlie, Erinna F. Lee, Christine J. HawkinsCell Signaling Technology, Danvers, MA, USA; 1:1000), BIK (Catalog #4592, Cell Signaling Technology; 1:1000), BID (Catalog #AF860, R&D Systems, Minneapolis, MN, USA; 1:400), PUMA (Catalog #ab9643, Abcam; 1:1000), BAD (Catalog #32445,

(3) Caspase-2 is essential for proliferation and self-renewal of nucleophosmin-mutated acute myeloid leukemiaScience advancesAugust 2, 2024Sakthivel D, Brown-Suedel AN, Lopez KE, Salgar S, Coutinho LE, Keane F, Huang S, Sherry KM, Charendoff CI, Dunne KP, et al.

(4) LINC complex protein nesprin-2 has pro-apoptotic activity via Bcl-2 family proteinsCell Death DiscoveryJanuary 15, 2024Liora Lindenboim, Hila Zohar, Gregg G. Gundersen, Howard J. Worman, Reuven Steinanti-Bcl-w (#MC-501) (Kamiya Biomedical Company) (dilution 1:1000 for immunoblotting), goat anti-Bid (#AF860) (R&D Systems) (dilution 1:10,000 for immunoblotting), rabbit anti-Mcl-1 (#600-401-394) (Rockland) (dilution

(5) Caspase-2 is essential for proliferation and self-renewal of nucleophosmin-mutated acute myeloid leukemiabioRxiv : the preprint server for biologyMay 30, 2023Sakthivel D, Brown-Suedel AN, Keane F, Huang S, Sherry KM, Charendoff CI, Dunne KP, Robichaux DJ, Le B, Shin CS, et al.

(6) High Levels of Progesterone Receptor B in MCF-7 Cells Enable Radical Anti-Tumoral and Anti-Estrogenic Effect of ProgestinBiomedicinesAugust 2, 2022Bajalovic N, Or YZ, Woo ARE, Lee SH, Lin VCL.(Cell Signaling Technology, Danvers, MA, USA, LOT number #9542), BID (R&D Systems, MN, USA, LOT number AF860), BAX (Cell Signaling Technology, Danvers, MA, USA, LOT number #2772), BCL-2 (Santa Cruz Biotechnology Inc

(7) An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic MacrophagesSSRN Electronic JournalApril 2, 2022Nathalia M. de Vasconcelos, Nina Van Opdenbosch, Hanne Van Gorp, Rosa Martín-Pérez, Annalisa Zecchin, Peter Vandenabeele, Mohamed Lamkanfianti-ROCKI Abcam Cat# ab45171; RRID: AB_2182005; clone EP786Y Goat polyclonal anti-Bid R&D systems Cat# AF860; RRID: AB_2065622 Goat polyclonal anti-mouse, HRPconjugated Jackson Immunoresearch Laboratories Cat#

(8) Modular immune-homeostatic microparticles promote immune tolerance in mouse autoimmune modelsScience Translational MedicineMarch 10, 2021Xin Chen, Xiaoshan Yang, Pingyun Yuan, Ronghua Jin, Lili Bao, Xinyu Qiu, Siying Liu, Tao Liu, John Justin Gooding, WanJun Chen, et al.(dilute at 1:1000; Cell Signaling Technology, 2870), and BID antibody (dilute at 1:1000; R&D Systems, AF860, lot: CDK031909A), were used for Western blotting. T cell culture For lymphocyte cultures, mice were

(9) p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury ModelCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Buitrago-Molina LE, Marhenke S, Becker D, Geffers R, Itzel T, Teufel A, Jaeschke H, Lechel A, Unger K, Markovic J, et al.BCL2-like 1 (610211; BD Biosciences), Bcl-2 homology 3 interacting domain death agonist at 1:2000 (AF860; Bio-Techne GmbH, formerly R&D Systems, Wiesbaden-Nordenstadt, Germany), BCL2-like 11 (apoptosis facilitator)

(10) Plants expressing murine pro-apoptotic protein Bid do not have enhanced PCDBMC research notesSeptember 21, 2020Manara A, Imanifard Z, Fracasso L, Bellin D, Crimi M.

(11) An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic MacrophagesCell reportsJuly 28, 2020de Vasconcelos NM, Van Opdenbosch N, Van Gorp H, Martín-Pérez R, Zecchin A, Vandenabeele P, Lamkanfi M.anti-ROCKI,Abcam,Cat# ab45171; RRID: AB_2182005; clone EP786Y Goat polyclonal anti-Bid,R&D systems,Cat# AF860; RRID: AB_2065622 "Goat polyclonal anti-mouse, HRP-conjugated",Jackson Immunoresearch Laboratories,Cat#

(12) Phenotypic selection with an intrabody library reveals an anti-apoptotic function of PKM2 requiring Mitofusin-1PLoS biologyJune 1, 2019Liu T, Kuwana T, Zhang H, Vander Heiden MG, Lerner RA, Newmeyer DD.antibody (Santa Cruz N20), anti-Bak antibody (Cell Signaling 3814), anti-Bid antibody (R&D Systems AF860), anti-Bim antibody (Sigma B7929), anti-Puma antibody (Cell Signaling 4976), antiBcl-2 antibody (Abcam

(13) Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane ruptureCell death and differentiationJanuary 1, 2019de Vasconcelos NM, Van Opdenbosch N, Van Gorp H, Parthoens E, Lamkanfi M.(9662S, Cell Signaling Technology) and anti-β-Actin-HRP (sc-47778, Santa Cruz Biotechnology), anti-BID (AF860, R&D Systems). Horseradish peroxidase (HRP)-conjugated secondary antibodies were acquired from Jackson

(14) Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolismCell death & diseaseOctober 9, 2018Naghdi S, Slovinsky WS, Madesh M, Rubin E, Hajnóczky G.Abcam), anti-Bax (N-20, sc-493, SantaCruz), anti-MCL-1 (ADI-AAP-240, Enzo life sciences), anti-Bid (no. AF860, R&D systems), anti-Bim (no.2933, Cell Signaling), anti-Bcl-xL (no. 610211, BD transduction), anti-Actin

(15) Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics studyeLifeOctober 3, 2018Salisbury-Ruf CT, Bertram CC, Vergeade A, Lark DS, Shi Q, Heberling ML, Fortune NL, Okoye GD, Jerome WG, Wells QS, et al.FHA-BidBH3 construct; Zinkel Laboratory; See Table 1 for primer sequences Antibody Bid (goat polyclonal) R and D systems AF860 RRID: AB_2065622 1:1000 (5% milk, Western Blot (WB)) Antibody Bid (rabbit polyclonal) PMID: 8918887 Antibody

(16) The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3CellNovember 16, 2017Gaidt MM, Ebert TS, Chauhan D, Ramshorn K, Pinci F, Zuber S, O'Duill F, Schmid-Burgk JL, Hoss F, Buhmann R, et al.,Santa Cruz Biotechnology,Cat#sc-2004 Human IL-1 beta /IL-1F2 Antibody,R&D Systems,Cat#AF-201-NA Human/Mouse BID Antibody,R&D Systems,Cat#AF860 IRF-3 (D83B9) Rabbit mAb,Cell Signaling Technology,Cat#4302 Mouse IL-1 beta /IL-1F2 Antibody,R&D Systems,Cat#AF-401-NA MUTED Antibody

(17) Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristineCell death & diseaseOctober 5, 2017Miles MA, Hawkins CJ.USA; M037-3), mouse anti-FLAG (M2) (Sigma; #3165), goat anti-Bid (R&D Systems, Minneapolis, MN, USA; AF860), mouse anti-caspase-3 (clone 19) (BD Biosciences, San Jose, CA, USA; #610323), rabbit anti-caspase-7

(18) Survivin, a key player in cancer progression, increases in obesity and protects adipose tissue stem cells from apoptosisCell death & diseaseMay 18, 2017Ejarque M, Ceperuelo-Mallafré V, Serena C, Pachón G, Núñez-Álvarez Y, Terrón-Puig M, Calvo E, Núñez-Roa C, Oliva-Olivera W, Tinahones FJ, et al.(#2772 and #9664, respectively; Cell Signaling Technology, Beverly, MA, USA); anti-tBID and anti-FLIP (AF860 and AF821, respectively; R&D Systems); anti-GAPDH (MA5-15738; Sigma-Aldrich); anti-FAA and anti-Ub (sc-66223

(19) Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like propertiesPloS oneJanuary 1, 2017Lacoste B, Raymond VA, Cassim S, Lapierre P, Bilodeau M.

(20) Subcellular localization of PUMA regulates its pro-apoptotic activity in Burkitt's lymphoma B cellsOncotargetNovember 10, 2015Ambroise G, Portier A, Roders N, Arnoult D, Vazquez A.Pharmingen; FLAG (F1804, clone M2) and GAPDH (G9545) from Sigma-Aldrich; NOXA (IMG-349A) from Imgenex; Bid (AF860) from R&D Systems; Bak (38145) caspase-3 (9662) and P-p38 (9211) from Cell Signaling Technology. Anti-human

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BID Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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BID Antibody from R&D Systems, a Bio-Techne Brand

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