CD47 N-terminal IgV-like Extracellular Domain Antibody AF1866 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD47 N-terminal IgV-like Extracellular Domain Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1866
NCBI Gene AliasesB430305P08Rik, 9130415E20Rik, Itgp, MER6, OA3, IAP
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunofluorescence,Immunocytochemistry
Conjugate/TagUnconjugated
HostGoat
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Rat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetCD47
NCBI Full Gene NameCD47 molecule, CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like, Gln19-Pro158, Accession # NP_034711
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
IsotypeIgG

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CD47 N-terminal IgV-like in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human CD47 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancerMolecular OncologyApril 20, 2026Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thuantibodies on lysates prepared with RIPA buffer from serum‐starved cells: mouse CD47 (1 : 1000, R&D Systems AF1866), human CD47 (#63000; Cell Signaling Technologies, 1 : 1000), p44/42 MAPK (Erk1/2) (#4696S; Cell Signaling Technologies

(2) Dual-mode action of scalable, high-quality engineered stem cell-derived SIRPα-extracellular vesicles for treating acute liver failureNature communicationsFebruary 23, 2025Kim S, Kim YK, Kim S, Choi YS, Lee I, Joo H, Kim J, Kwon M, Park S, Jo MK, et al.at room temperature. The primary antibodies [pMLKL (1:200, Abcam, ab196436), CD47 (1:40, R&D system, AF1866), and F4/80 (1:200, Bio-Rad, MCA497)] were applied to stain slides using a 1% BSA/PBS solution. After

(3) Smooth muscle cell-specific CD47 deletion suppresses atherosclerosisLife SciencesJanuary 15, 2025Naveed Pervaiz, Rashid Mehmood, Ravi Varma Aithabathula, Ishita Kathuria, WonMo Ahn, Britney-Thuy Le, Ki-Suk Kim, Udai P. Singh, Gabor Csanyi, Bhupesh Singlaovernight at 4°C with primary antibodies against ACTA2 (Abcam, #ab7817, 1:100), CD47 (R&D Systems, #AF1866, 1:100), TSP1 (Abcam, #ab85762, 1:80) and CD68 (ThermoFisher Scientific, #MA5–13324, 1:100). Afterward,

(4) Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infectionNature communicationsApril 30, 2024Moon S, Han S, Jang IH, Ryu J, Rha MS, Cho HJ, Yoon SS, Nam KT, Kim CH, Park MS, et al.

(5) CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunityNature immunologyDecember 1, 2023Tang Z, Zhong MC, Qian J, Galindo CC, Davidson D, Li J, Zhao Y, Hui E, Veillette A.utilized anti-Flag MAb M2 (Sigma-Aldrich), anti-Myc MAb 9B11 (Cell Signaling Technology), anti-mCD47 MAb AF1866 (R&D Systems), anti-mSLAMF7 rabbit antiserum (generated in our laboratory), anti-hCD47 MAb D3O7P (Cell

(6) EGFR-Induced and c-Src-Mediated CD47 Phosphorylation Inhibits TRIM21-Dependent Polyubiquitylation and Degradation of CD47 to Promote Tumor Immune EvasionAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Du L, Su Z, Wang S, Meng Y, Xiao F, Xu D, Li X, Qian X, Lee SB, Lee JH, et al.China). Polyclonal antibodies against human CD47 (AF4670, 1 µg mL−1 for immunoblotting) and mouse CD47 (AF1866, 1 µg mL−1 for immunoblotting) were obtained from R&D Systems (Minneapolis, MN). A monoclonal antibody

(7) Engineering Human Pluripotent Stem Cell Lines to Evade Xenogeneic Transplantation BarriersbioRxiv : the preprint server for biologyJune 29, 2023Pizzato HA, Alonso-Guallart P, Woods J, Johannesson B, Connelly JP, Fehniger TA, Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D.

(8) Macrophage LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Is Required for the Effect of CD47 Blockade on Efferocytosis and Atherogenesis-Brief ReportArteriosclerosis, thrombosis, and vascular biologyJanuary 1, 2022Mueller PA, Kojima Y, Huynh KT, Maldonado RA, Ye J, Tavori H, Pamir N, Leeper NJ, Fazio S.transferred to nitrocellulose membrane. CD47 was detected with goat anti-CD47 (0.25mg/mL; R&D Systems; Cat# AF1866) with a smear containing multiple bands stretching from 40kD to 50kD resulting from the presence of

(9) Loss of microglial SIRPα promotes synaptic pruning in preclinical models of neurodegenerationNature communicationsApril 1, 2021Ding X, Wang J, Huang M, Chen Z, Liu J, Zhang Q, Zhang C, Xiang Y, Zen K, Li L.antibodies overnight at 4 °C. Primary antibodies were used as follows: goat anti-CD47 (1:1000, R&D Systems, AF1866), rabbit anti-SIRPα (1:5000, Abcam, ab8120), rabbit anti SIRPB1 (1:2000, LifeSpan Biosciences, LS‑C679465),

(10) Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivoMucosal immunologyMarch 1, 2021Azcutia V, Kelm M, Luissint AC, Boerner K, Flemming S, Quiros M, Newton G, Nusrat A, Luscinskas FW, Parkos CA.E-selectin-Fc, VCAM-1-Fc and ICAM-1-Fc chimera molecules, and the polyclonal goat anti-mouse CD47 (cat#AF1866) and goat anti-human JAM-A (cat#AF1077) antibodies were from R&D Systems (Minneapolis, MN). Recombinant

(11) Epithelial CD47 is critical for mucosal repair in the murine intestine in vivoNature communicationsNovember 1, 2019Reed M, Luissint AC, Azcutia V, Fan S, O'Leary MN, Quiros M, Brazil J, Nusrat A, Parkos CA.Novus Biologicals: Anti-TGF-b1 (clone 7F6; NBP2-22114; d:1/250). From R&D systems: Anti-mouse CD47 (AF1866; WB:1/2000, IF:1/100), Anti-mouse E-Cadherin (AF748; IF:1/100), Rat anti- mouse Integrin beta 1 (MAB2405;

(12) CD47-blocking antibodies restore phagocytosis and prevent atherosclerosisNatureAugust 4, 2016Kojima Y, Volkmer JP, McKenna K, Civelek M, Lusis AJ, Miller CL, Direnzo D, Nanda V, Ye J, Connolly AJ, et al.

(13) Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancerInternational journal of cancerJuly 1, 2013Vermeer DW, Spanos WC, Vermeer PD, Bruns AM, Lee KM, Lee JH.

(14) CD47 gene knockout protects against transient focal cerebral ischemia in miceExperimental NeurologyMay 1, 2009Guang Jin, Kiyoshi Tsuji, Changhong Xing, Yong-Guang Yang, Xiaoying Wang, Eng H. Loloaded per lane. The primary antibody was a 1:1000 dilution of goat polyclonal antibody against CD47 (AF1866, R&D Systems). Western blots were performed with horseradish peroxidase (HRP)-conjugated anti-goat IgG

(15) Raft aggregation with specific receptor recruitment is required for microglial phagocytosis of Abeta42GliaFebruary 1, 2009Persaud-Sawin DA, Banach L, Harry GJ.incubation with anti-mouse CD36 (cat# 100011, Cayman Chemical, Ann Arbor MI; 1:200) or anti-mouse CD47 (cat# AF1866, R&D systems, Minneapolis, MN; 1:100) for 1 hr. Cells were washed and incubated with an Alexafluor 350

(16) CD47 prevents the elimination of diseased fibroblasts in sclerodermaJCI insightJanuary 1, 0001"Lerbs T, Cui L, King ME, Chai T, Muscat C, Chung L, Brown R, Rieger K, Shibata T, Wernig G."(Dako, m0823), CD47 (Thermo Fisher Scientific, 14-0479-82, clone B6H12), CD47 (R&D Systems, Bio-Techne, AF1866), CD68 (Agilent, GA60691-2, clone KP1), cleaved caspase-3 (CST, 96645, clone 5A1E), FSP1 (MilliporeSigma,

(17) Neuronal signal-regulatory protein alpha drives microglial phagocytosis by limiting microglial interaction with CD47 in the retinaImmunityJanuary 1, 0001"Jiang D, Burger CA, Akhanov V, Liang JH, Mackin RD, Albrecht NE, Andrade P, Schafer DP, Samuel MA."monoclonal anti-CD47,BD Biosciences,Cat#555297; RRID:AB 395713 Goat polyclonal anti-CD47,R&D Systems,Cat#AF1866; RRID:AB 2074942 Chicken polyclonal anti-GFP,Abcam,Cat#ab13970; RRID:AB 300798 Rabbit polyclonal anti-Vglut1,Synaptic Systems

(18) CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis"Arteriosclerosis, thrombosis, and vascular biology"January 1, 0001"Singla B, Aithbathula RV, Pervaiz N, Kathuria I, Swanson M, Ekuban FA, Ahn W, Park F, Gyamfi M, Cherian-Shaw M, et al."(Thermo Fisher Scientific; 53-0031-80, 1:80), and CD47 (Novus Biologicals; NBP2-31106, 1:100 or R&D Systems; AF1866, 1:70) overnight at 4 °C. Next, sections were incubated with Alexa Fluor 488/Alexa Fluor 594–labeled

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Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antibody

CD47 N-terminal IgV-like Extracellular Domain Antibody

CD47 N-terminal IgV-like Extracellular Domain Antibody

CD47 N-terminal IgV-like Extracellular Domain Antibody

CD47 N-terminal IgV-like Extracellular Domain Antibody

CD47 N-terminal IgV-like Extracellular Domain Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (18)

Reviews

CD47 N-terminal IgV-like Extracellular Domain Antibody from R&D Systems, a Bio-Techne Brand

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