Mouse RANK/TNFRSF11A Antibody AF692 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse RANK/TNFRSF11A Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF692
NCBI Gene AliasesODFR, OFE, Rank, mRANK, Ly109, TRANCE-R
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Agonist Activity,Neutralization,Functional Assay
IsotypeIgG
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Transgenic Mouse
TargetRANK/TNFRSF11A
NCBI Full Gene Nametumor necrosis factor receptor superfamily, member 11a, NFKB activator
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse RANK/TNFRSF11A, Gln30-Pro213, Accession # O35305
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used to study thymic epithelial cells in mice?

Yes, the RANK/TNFRSF11A antibody has been utilized in studies examining thymic epithelial cells. For instance, in a study by Baik et al. (2013), the antibody was added to thymus organ cultures to investigate the role of RANK in the development of thymic epithelial compartments.

Is there any evidence that this antibody can help in understanding the role of RANK in salivary gland tumors?

Yes, the RANK/TNFRSF11A antibody has been referenced in research focused on salivary gland tumors. Szwarc et al. (2015) demonstrated that aberrant activation of the RANK signaling pathway contributes to tumor development in murine models, indicating the antibody's relevance in studying this signaling axis in cancer.

How effective is this antibody in flow cytometry applications?

The RANK/TNFRSF11A antibody has been shown to work effectively in flow cytometry applications. Danzl et al. (2014) utilized the antibody in their experiments to analyze thymic epithelial cell populations, confirming its utility in flow cytometric analysis.

What is the specificity of this antibody regarding mouse and human RANK?

The RANK/TNFRSF11A antibody specifically detects mouse RANK/TNFRSF11A with approximately 20% cross-reactivity with recombinant human RANK in direct ELISAs, making it suitable for studies primarily focused on mouse models while allowing for some comparative analysis with human RANK.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Acute Extrinsic Activation of the RANKL Pathway Decreases Wound Healing and Functional Recovery After Spinal Cord Injury in MiceGliaMay 1, 2025A. L. Vivinetto, A. Bernstein, M. Soliman, J. W. Cave, E. HollisThermo Fisher Scientific Cat# PA1-10004, RRID:AB_1074620), RANKL (1:500, goat, R and D Systems Cat# AF692, RRID:AB_2205357), and Sox-9 (1:500, goat, R and D Systems Cat# AF3075, RRID:AB_2194160). Primary astrocyte culture

(2) Mechanosensitive hormone signaling promotes mammary progenitor expansion and breast cancer riskCell stem cellJanuary 4, 2024Northey JJ, Hayward MK, Yui Y, Stashko C, Kai F, Mouw JK, Thakar D, Lakins JN, Ironside AJ, Samson S, et al.

(3) Differential osteoblastic activity in primary metaphyseal trabecular and secondary trabeculae of c-fos deficient miceJournal of oral biosciencesDecember 1, 2023Yamamoto T, Abe M, Hongo H, Maruoka H, Yoshino H, Haraguchi-Kitakamae M, Udagawa N, Li M, Amizuka N, Hasegawa T.

(4) RANK links senescence to stemness in the mammary epithelia, delaying tumor onset but increasing tumor aggressivenessDevelopmental cellJune 21, 2021Benítez S, Cordero A, Santamaría PG, Redondo-Pedraza J, Rocha AS, Collado-Solé A, Jimenez M, Sanz-Moreno A, Yoldi G, Santos JC, et al.pressure cooker (20 min) prior to overnight antibody incubation at 4°C. Anti-mouse Rank (R&D Systems AF692) immunostaining was performed pretreating sections with Protease XXIV 5 U/ml (Sigma-Aldrich) for 5 min

(5) Prevention of Acute Lung Injury by a Novel CD14-Inhibitory Receptor Activator of the NF-κB Ligand Peptide in MiceImmunoHorizonsJune 15, 2021Ju N, Hayashi H, Shimamura M, Yoshida S, Nakamaru R, Nakagami H, Morishita R, Rakugi H.Western blotting, and immunostaining included the following: anti-CD14 (EPR21847; Abcam), anti-RANK (AF692; R&D Systems), anti–phospho-IRF3 (Ser396) (4D4G) (Cell Signaling Technology), anti–NF-kB p65 (D14E12)

(6) Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongationNature cell biologyApril 1, 2019Romeo SG, Alawi KM, Rodrigues J, Singh A, Kusumbe AP, Ramasamy SK.

(7) RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesisJournal of translational medicineMarch 15, 2019Jhun J, Lee SH, Kim SY, Ryu J, Kwon JY, Na HS, Jung K, Moon SJ, Cho ML, Min JK.

(8) Receptor activator of nuclear factor κB expression is a prognostic factor in human osteosarcomaOncology lettersSeptember 1, 2015Trieb K, Windhager R.h at room temperature. Mouse RANK affinity purified polyclonal antibody, goat IgG (1:500; cat. no. AF692) and isotype control polyclonal goat IgG1 (1:250; cat. no. AF108) (R&D Systems, Inc., Minneapolis, MN,

(9) Aberrant Activation of the RANK Signaling Receptor Induces Murine Salivary Gland TumorsPloS oneJanuary 1, 2015Szwarc MM, Kommagani R, Jacob AP, Dougall WC, Ittmann MM, Lydon JP.previously reported [15]. Primary antibodies for the following antigens used in these studies were: RANK ((AF692) 1:200 dilution; R&D Systems, Inc., Minneapolis, MN); RANKL ((AF462) 1:200 dilution; R&D Systems, Inc.);

(10) OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in miceProceedings of the National Academy of Sciences of the United States of AmericaJune 3, 2014Shimamura M, Nakagami H, Osako MK, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R.

(11) MHC class II transactivator is an in vivo regulator of osteoclast differentiation and bone homeostasis co-opted from adaptive immunityJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchFebruary 1, 2014Benasciutti E, Mariani E, Oliva L, Scolari M, Perilli E, Barras E, Milan E, Orfanelli U, Fazzalari NL, Campana L, et al.

(12) Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6PloS oneJanuary 1, 2014Danzl NM, Jeong S, Choi Y, Alexandropoulos K.g/L glucose and L-glutamine and 10% FCS in the presence or absence of stimulating anti-RANK antibody (AF692, R&D Systems, 10 µg/mL) as described [32]. At the end of the incubation, cells were stained with anti-MHCII-eFluor450,

(13) Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parityAutophagyJanuary 1, 2014Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V.from Leica, ESR1/ERα (sc-542) and RELA/p65 (sc-8008) from Santa Cruz Biotechnology, TNFRSF11A/TNR11 (AF692) and TNFSF11/TNF11 (AF462) from R&D Systems, SQSTM1 (BML-PW9860) from Enzo, LC3 (NB100-2331) and IL1B/IL-1β

(14) Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitorsEuropean journal of immunologyMarch 1, 2013Baik S, Jenkinson EJ, Lane PJ, Anderson G, Jenkinson WE.dissected following physical removal of the parathyroid. A total of 10 μg/mL agonistic anti-RANK antibody (AF692, R&D Systems) was added to cultures where indicated 13. Immunohistochemistry Thymus grafts were processed

(15) The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouseDevelopmental biologyApril 1, 2009Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP.

(16) The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosisJournal of immunology (Baltimore, Md. : 1950)March 15, 2007Kovacić N, Lukić IK, Grcević D, Katavić V, Croucher P, Marusić A.

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RANK/TNFRSF11A Antibody

RANK/TNFRSF11A Antibody

RANK/TNFRSF11A Antibody

RANK/TNFRSF11A Antibody

Mouse RANK/TNFRSF11A Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (16)

Reviews

Mouse RANK/TNFRSF11A Antibody from R&D Systems, a Bio-Techne Brand

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