VEGFR3/Flt-4 Antibody AF743 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemVEGFR3/Flt-4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF743
ApplicationsELISA Development,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,CyTOF-ready
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
HostGoat
TargetVEGFR3/Flt-4
Quantity100 ug (also 25 ug)
ReactivityHu, Ms, Rt, CyMk, Hr
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGFR3/Flt-4, Tyr25-Asp770, Accession # P35917
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namefms related receptor tyrosine kinase 4, FMS-like tyrosine kinase 4
NCBI Gene AliasesFLT-4, FLT41, CHTD7, VEGFR3, LMPHM1, PCL, Chy, LMPH1A, Flt-4, VEGFR-3

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse VEGFR3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) VEGFR3 is observed and less than 5% cross-reactivity with recombinant mouse VEGFR2 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study lymphatic endothelial cells in mouse models?

Yes, the VEGFR3/Flt-4 antibody (AF743) has been utilized in various studies involving lymphatic endothelial cells in mouse models. For instance, it was used to characterize podoplanin-positive lymphatic endothelial cells in flow cytometry assays, confirming its effectiveness in identifying these cells in vivo ().

Is there any evidence of this antibody being used in research related to lymphangiogenesis?

The VEGFR3/Flt-4 antibody has been shown to play a role in studies of lymphangiogenesis. For example, it was employed in a study that investigated the effects of double-stranded RNA mimics on neolymphangiogenesis, highlighting its relevance in understanding lymphatic vessel development and function ().

How has this antibody been applied in the context of aging and lymphatic function?

The antibody has been used in research examining the impact of aging on lymphatic function. In one study, it was utilized to assess the expression of VEGFR-3 in lymphatic endothelial cells, revealing that aging decreases VEGFR-3 signaling, which is crucial for maintaining lymphatic vessel density and function ().

What specific applications has this antibody been validated for in scientific literature?

The VEGFR3/Flt-4 antibody has been validated for use in several applications, including immunohistochemistry, flow cytometry, and Western blotting. It has been cited in studies focusing on lymphatic vessel characterization and the signaling pathways involved in lymphangiogenesis and vascular development (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) VEGFR2 is required for VEGF-C-VEGFR3-PI3Kα-mediated sprouting lymphangiogenesisNat CommunMay 15, 2026Hans Schoofs, Yan Zhang, Henrik Ortsäter, Mariya Lytvyn, Rui Benedito, Taija MäkinenSamples were subsequently stained using the following antibodies: goat anti-mouse VEGFR3 (1:100) (#AF743, R&D Systems), goat anti-mouse VEGFR2 (1:100) (#AF644, R&D Systems), rat anti-mouse LYVE1 (1:200) (#MAB2125,

(2) Macrophage-mediated refinement of the dural lymphatic regulates social behaviorNeuronApril 24, 2026Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu, et al.,Thermo Fisher Scientific,"Cat# 53-5381-82, RRID:AB_1106990" Goat polyclonal anti-VEGFR3,R&D Systems,"Cat# AF743, RRID:AB_355563" Mouse monoclonal anti-Claudin 5-A488,Thermo Fisher Scientific,"Cat# 352588, RRID:AB_2532189" Goat

(3) NF-κB–driven lymphangiogenesis affects kidney function via a VEGFR-3–mediated pathwayJCI InsightMarch 9, 2026Arin L. Melkonian, Amie M. Traylor, Anna A. Zmijewska, Kyle H. Moore, Gelare Ghajar-Rahimi, Stephanie Esman, Yanlin Jiang, Hani Jang, Babak J. Mehrara, Timmy C. Lee, et al.separated by SDS-PAGE and probed with the following antibodies: VEGFR3/Flt-4 (1:1,000; R&D Systems, AF743), mouse Podoplanin (1:5,000; R&D Systems, AF3244), Prox1 (1:1,000; Abcam, ab199359), Lyve-1 (1:800;

(4) Cooperative ETS transcription factors are required for lymphatic endothelial cell integrity and resilienceJournal of Clinical InvestigationMarch 2, 2026Myung Jin Yang, Seok Kang, Seon Pyo Hong, Hokyung Jin, Jin-Hui Yoon, Cheolhwa Jin, Chae Min Yuk, Lydia Getachew Gebeyehu, Junho Jung, Sung-Hwan Yoon, et al.AF457, R&D Systems); anti-CD31 (hamster monoclonal, MAB1398Z, Merck); anti-VEGFR3 (goat polyclonal, AF743, R&D Systems); anti-LYVE1 (rabbit polyclonal, 11-034, Angiobio); anti-Reelin (goat polyclonal, AF3820,

(5) Cooperative ETS transcription factors are required for lymphatic endothelial cell integrity and resilienceJ Clin InvestDecember 23, 2025Myung Jin Yang, Seok Kang, Seon Pyo Hong, Hokyung Jin, Jin-Hui Yoon, Cheolhwa Jin, Chae Min Yuk, Lydia Getachew Gebeyehu, Junho Jung, Sung-Hwan Yoon, et al.AF457, R&D Systems); anti-CD31 (hamster monoclonal, MAB1398Z, Merck); anti-VEGFR3 (goat polyclonal, AF743, R&D Systems); anti-LYVE1 (rabbit polyclonal, 11-034, Angiobio); anti-Reelin (goat polyclonal, AF3820,

(6) Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down SyndromeImmunologyNovember 27, 2025Koki Harada, Keiichi Ishihara, Sayaka Wakayama, Teruhiko Wakayama, Sae Yamamoto, Haruhiko Sago, Shun Shimohama, Satoshi Akiba, Florent Ginhoux, Kazuyuki Takataanti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Mouse IgG2B Isotype Control (1:200, R&D Systems, MAB004) and Normal Goat IgG Control (1:40, R&D Systems,

(7) Site-1 protease–mediated cholesterol metabolism is essential for lymphatic development in miceJCI InsightOctober 22, 2025Yuji Kondo, Yizhi Jiang, Xin Geng, Jianhua Song, Summer Simeroth, J. Michael McDaniel, Pengchun Yu, R. Sathish Srinivasan, Lijun Xiaused as a control. Antibodies and chemicals. Antibodies against VEGFR3 (13-5988-82, eBioscience, or AF743, R&D Systems), CD31 (2H8, in-house), Alexa Fluor 647–conjugated CD31 (102515, BioLegend), Alexa Fluor

(8) Acute and chronic nasal inflammation induces lymphangiogenesis in the olfactory mucosa in miceBrain Behav Immun HealthOctober 7, 2025Suzuho Komaki, Ryuichi Imai, Yuzuki Sugimoto, Rei Settsu, Aki Obara, Atsuyoshi Shimada, Robert Dantzer, Geoffroy Laumet, Fumiaki Imamura, Sanae Hasegawa-IshiiBuffer) Ly6G,rat,AdipoGen,1:200,AG-20 B-0043 PF,No LYVE-1,goat,R&D Systems,1:500,AF2125,No VEGFR-3,goat,R&D Systems,1:500,AF743,No Prox-1,goat,R&D Systems,1:1000,AF2727,Yes (Tris-EDTA Buffer) Ki-67,rat,Invitrogen,1:500,14-5698-82,Yes

(9) Three-dimensional modelling of lymphangiogenesis in-vitro using bioorthogonal click-crosslinked gelatin hydrogelsMater Today BioOctober 1, 2025Dana E. Al-Ansari, Yangshuo Hu, Nicola Contessi Negrini, Daisy Jones, Graeme M. Birdsey, Adam D. Celizincubation with anti-CD31 antibody (Abcam, #Ab28364, 1:200 dilution) or anti-FLT4 antibody (R&D Systems, #AF743, 1:100 dilution) for 18 h at 4 °C. The slides were then washed with 0.05 % PBST followed by 1 h incubation

(10) Lymphatic endothelial mTORC1 instructs metabolic and developmental signaling during lymphangiogenesisDevelopmental CellSeptember 8, 2025Fei Han, Summer Simeroth, Jie Zhu, Irma Gryniuk, Atul Pranay, Weiqing Chen, Yuan Wang, Yuanyuan Cai, Zhiyuan Shen, Guangyu Wang, et al.mounting. The following primary antibodies were used: PECAM1 (BD Pharmingen, 553370), VEGFR3 (R&D Systems, AF743), PROX1 (Angiobio, 11–002), PROX1 (R&D Systems, AF2727), NRP2 (R&D Systems, AF567), PH3 (Sigma-Aldrich,

(11) Inhibition of VEGFR-3 by SAR131675 decreases renal inflammation and lymphangiogenesis in the murine lupus nephritis modelCell Death DiscovJuly 12, 2025Tian Wang, Wenjia Li, Ji-Hyun Yeom, Zhiheng Liu, Kyoung Min Kim, Kyung Pyo Kang1:100; Abcam), a rabbit anti-VEGF-C (PA5-29772; dilution 1:200; Invitrogen), a gout anti-mouse VEGFR-3 (AF743; dilution 1:100; R&D systems), a rabbit anti-mouse CD21 (ab75985; dilution 1:250; Abcam), a rat anti-mouse

(12) Increased CSF drainage by non-invasive manipulation of cervical lymphaticsNatureJune 4, 2025Hokyung Jin, Jin-Hui Yoon, Seon Pyo Hong, Yu Seok Hwang, Myung Jin Yang, Jieun Choi, Hae Jin Kang, Seung Eun Baek, Cheolhwa Jin, Junho Jung, et al.used were: anti-mouse LYVE1 (rabbit polyclonal; 11-034, Angiobio); anti-mouse VEGFR3 (goat polyclonal; AF743, R&D Systems); anti-mouse αSMA-Cy3 (mouse monoclonal, clone 1A4; C6198, Sigma); anti-mouse laminin α5

(13) Exercise-Induced Cardiac Lymphatic Remodeling Mitigates Inflammation in the Aging HeartAging cellJune 1, 2025Roh K, Li H, Freeman RN, Zazzeron L, Lee A, Zhou C, Shen S, Xia P, Guerra JRB, Sheffield C, et al.anti‐LYVE‐1 (1:100, #11–034, Angiobio), anti‐LYVE‐1 (1:100, #MAB2125‐100, R&D SYSTEMS), anti‐VEGFR3 (1:100, #AF743, R&D SYSTEMS), anti‐VE‐Cadherin (1:100, # AF1002, R&D SYSTEMS), anti‐Ki‐67 (1:50, #14–5698‐82, eBioscience),

(14) Embryological cellular origins and hypoxia-mediated mechanisms in PIK3CA-driven refractory vascular malformationsEMBO Molecular MedicineApril 16, 2025Sota Torii, Keiki Nagaharu, Nanako Nakanishi, Hidehito Usui, Yumiko Hori, Katsutoshi Hirose, Satoru Toyosawa, Eiichi Morii, Mitsunaga Narushima, Yoshiaki Kubota, et al.Prox1 (11-002, AngioBio, 1:100, RRID:AB_10013720; AF2727, R&D Systems, 1:100, RRID:AB_2170716), VEGFR3 (AF743, R&D Systems, 1:100, RRID:AB_355563), GFP (ab290, Abcam, 1:250, RRID:AB_303395), phospho-S6 (#2215,

(15) Epicardial VEGFC/D signaling is essential for coronary lymphangiogenesisEMBO ReportsMarch 24, 2025Ester de la Cruz, Vanessa Cadenas, Susana Temiño, Guillermo Oliver, Miguel TorresSM22α abcam ab14106, Rabbit 1:200; VEGFD Cusabio CSB-PA07554A0Rb, Rabbit 1:200; VEGFR3 R&D Systems AF743, Goat 1:100/1:200; CXCL12 R&D Systems MAB350, Mouse 1:100. Whole-mount immunofluorescence For hearts,

(16) Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisectionsBone ResJanuary 13, 2025Yuehan Ni, Jiamiao Wu, Fengqi Liu, Yating Yi, Xiangjiao Meng, Xiang Gao, Luyi Xiao, Weiwei Zhou, Zexi Chen, Peng Chu, et al.,Santa Cruz,sc-65495 Goat anti-endomucin,1 ng/μL,R&D Systems,AF4666 Goat anti-VEGFR3/Flt-4,1:100,R&D Systems,AF743 Goat anti-VE-Cadherin,1:100,R&D Systems,AF1002 Rabbit anti-DsRed,1:250,Takara Bio,632496 Rabbit anti-laminin

(17) Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expressioniScienceDecember 20, 2024Kako Hanada, Yuki Saito, Takahiro Takagi, Mitsuki Go, Yota Nakano, Toshihiko Inagawa, Hideyo Hirai, Marcus Fruttiger, Susumu Itoh, Fumiko Itohmonoclonal anti-PECAM-1,BD Biosciences,Cat# 550274; RRID: AB_393571 Goat anti VEGFR3,R&D systems,Cat# AF743; RRID: AB_355563 "Mouse anti-Actin, alpha-Smooth Muscle – Cy3 ™",Millipore Sigma,Cat# C6198; RRID:

(18) Dynamic behavior and lineage plasticity of the pulmonary venous endotheliumNature Cardiovascular ResearchDecember 9, 2024Joanna Wong, Gan Zhao, Stephanie Adams-Tzivelekidis, Hongbo Wen, Prashant Chandrasekaran, Sylvia N. Michki, Maria E. Gentile, Madeline Singh, Sara Kass-Gergi, Meryl Mendoza, et al.600–401-379); anti-mouse RFP (goat, 1:250; Rockland, 200–101-379); anti-mouse VEGFR3 (goat, 1:200; R&D Systems, AF743); anti-mouse SYP (rabbit; Proteintech, 17785–1-AP); anti-mouse RAGE (rat, 1:500; R&D Systems, MAB1179–100);

(19) Specialized post-arterial capillaries facilitate adult bone remodellingNature cell biologyDecember 1, 2024Mohanakrishnan V, Sivaraj KK, Jeong HW, Bovay E, Dharmalingam B, Bixel MG, Dinh VV, Petkova M, Paredes Ugarte I, Kuo YT, et al.

(20) EphrinB2-mediated CDK5/ISL1 pathway enhances cardiac lymphangiogenesis and alleviates ischemic injury by resolving post-MI inflammationSignal transduction and targeted therapyNovember 18, 2024Bai Y, Chen L, Guo F, Zhang J, Hu J, Tao X, Lu Q, Li W, Chen X, Gong T, et al.antibodies utilized were as follows: EphrinB2 (10 μg/ml;# AF496, R&D system), VEGFR3/FLT4 (2 μg/ml; #AF743, R&D system), LYVE-1 (1:100; #67538, Cell Signal Technology), CD31 (1:100; #ab9498, Abcam), CD68 (1:100;

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