Mouse Mer Antibody AF591 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Mer Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $559.00Supplier Page View Company Product Page
Catalog NumberAF591
NCBI Gene AliasesNyk, Eyk, nmf12, Mer
ApplicationsActivation,IF/IHC,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Proximity Ligation Assay,In vivo assay,Neutralization,Immunofluorescence,Immunocytochemistry,CyTOF-reported,CyTOF-ready,Functional Assay,Cell Culture,Dual RNAscope ISH-IHC Compatible,ELISA Capture
IsotypeIgG
HostGoat
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rat,Transgenic Mouse
TargetMer
NCBI Full Gene NameMER proto-oncogene tyrosine kinase
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer, Glu23-Phe498, Accession # Q60805
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What applications has the Mer antibody been shown to work in, particularly in relation to phagocytic processes?

The Mer antibody has been utilized in various applications including immunofluorescence and immunohistochemistry, particularly in studies examining the roles of astrocytes and microglia in phagocytosis during neuronal corpse removal. It has been shown that MERTK signaling is critical for the engagement of microglia with dying neurons, highlighting its importance in maintaining brain homeostasis during phagocytic processes ().

Can the Mer antibody be used to study macrophage functions in disease models?

Yes, the Mer antibody has been employed in studies investigating macrophage functions in various disease contexts, including atherosclerosis. Research indicates that the receptor MerTK is involved in the regulation of efferocytosis, which is crucial for resolving inflammation and maintaining tissue homeostasis. Specifically, it has been shown that MerTK signaling is impaired in diabetic conditions, affecting macrophage-mediated clearance of apoptotic cells ().

Is the Mer antibody effective in studying the immune response to viral infections?

The Mer antibody has been used to explore the role of MerTK in the immune response to viral infections. Studies have demonstrated that MerTK signaling helps maintain blood-brain barrier integrity during neuroinvasive viral infections, suggesting that it plays a protective role against such infections by modulating immune responses ().

What is the specificity of the Mer antibody in terms of cross-reactivity with other proteins?

The Mer antibody is specific for mouse Mer, with approximately 20% cross-reactivity observed with recombinant human Mer in direct ELISAs. It shows less than 1% cross-reactivity with recombinant mouse Axl and Dtk, indicating a high level of specificity for its intended target ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Macrophage-mediated refinement of the dural lymphatic regulates social behaviorNeuronApril 24, 2026Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu, et al.,Thermo Fisher Scientific,"Cat# 13-0032-82, RRID:AB_2572762" Goat polyclonal anti-MerTK,R&D Systems,"Cat# AF591, RRID:AB_2098565" Goat polyclonal anti-Axl,R&D Systems,"Cat# AF854, RRID:AB_355663" Rat monoclonal

(2) Myeloid HDAC3 deletion protects against traumatic optic injuryCell Death DiscoveryMarch 18, 2026Rami A. Shahror, Carol A. Morris, Ashlynn Cunningham, Piyanan Chuesiang, Abdelrahman Y. Foudacryostat. Sections were then labeled for Iba-1, CD68 (BioLegend, Cat. #137002), MerTk (R&D Systems, Cat. # AF591), MBP (Abcam, Cat. # ab7349), HDAC3 (Proteintech, Cat. # 10255-1-AP), and GFAP (Abcam, Cat. #13-0300).

(3) Opposing functions of miR-155-5p and Socs1 drive vascular inflammation in diabetes-accelerated atherosclerosisCardiovascular DiabetologyFebruary 24, 2026María Kavanagh, Isabel Herrero del Real, Ignacio Prieto, Raquel Alvarez-Moreno, Jesus Egido, Oscar Lopez-Franco, Iolanda Lázaro, Carmen Gomez-GuerreroAB_869007) and Mer receptor tyrosine kinase (soluble form, sMerTK; 1:50 dilution; R&D Systems Cat# AF591, RRID: AB_2098565). Detection was performed with biotinylated secondary antibodies (1:200 dilution;

(4) Macrophages Are Critical Inducers of Bleomycin-Induced Fibrosis in a Systemic Scleroderma ModelThe American Journal of PathologyFebruary 1, 2026Blandine Baffert, Kevin Schneider, Audrey Wetzel, Ludivine Dal Zuffo, Cécile Chagué, Eléa Mitifiot, Francis Bonnefoy, Alicja Kuzniewska, Philippe Saas, Jamal Bamoulid, et al.transferred onto PVDF membrane. Western blotting was performed using a primary antibody against soluble Mer (AF591, R&D Systems, Minneapolis, MN, USA). Chemiluminescent signals were detected using the Western Lightning

(5) Mertk promotes early microglial-mediated synaptic engulfment in Alzheimer's diseaseTheranosticsJanuary 1, 2026Yifan Wu, Yuanteng Fan, Shisan Bao, Yinghao Song, Ruizhu Wang, Jiaxuan Wu, Xuan Liu, Jingwen Jin, Lingchen Kong, Baohua Hou, et al.(1:1000, ab178846, abcam, UK); Mouse anti-IBA1 (1:500, ab283319, abcam, UK), Goat anti-Mertk (1:200, AF591, R&D systems, USA); Rabbit anti-PSD95 (1:200, 51-6900, Invitrogen, USA); Mouse anti-Synapsin I (1:400,

(6) Spatial protein profiling reveals active roles for astrocytes in the chronic active lesion core during multiple sclerosisActa NeuropathologicaNovember 1, 2025Brandon C. Smith, Anthony Chomyk, Maria L. Habean, Benjamin C. Shaw, Rachel A. Tinkey, Bruce D. Trapp, Jessica L. Williams(Cell Signaling, 8753T), 4-1BB (ThermoFisher, 14-9056), Tim-3 (BioLegend, 119702), MERTK (R&D Systems, AF591), CTSD (Novus, MAB1029), TMEM119 (BioLegend, 853301), LAMP1 (Abcam, AB25630), and CD68 (Novus Biologicals,

(7) STK38 Kinase Promotes Cell Migration Induced by Oncogenic Ras via MerTK ActivationInternational Journal of Molecular SciencesOctober 25, 2025Satoshi Ohta, Kenji Tago, Katsumi Kasashima, Masayuki Ebina, Kaoru Tominagaproved with anti-α-tubulin (Merck Millipore, T6199), anti-MerTK (R&D systems [Minneapolis, MN, USA], AF591), anti-STK38 (Abnova corporation [Taipei, Taiwan], 00011329-MO2), anti-phosho-STK38 (Thermo Fisher Scientific,

(8) Pathology-oriented multiplexing enables integrative disease mappingNatureAugust 1, 2025Kuehl M, Okabayashi Y, Wong MN, Gernhold L, Gut G, Kaiser N, Schwerk M, Gräfe SK, Ma FY, Tanevski J, et al.

(9) PTP Inhibition Improves the Macrophage Antitumor Immune Response and the Efficacy of Chemo- and RadiotherapyCancer immunology researchMay 2, 2025Prieto-Dominguez N, Goel P, Ojo OA, Moretto K, Holtzhausen A, Humphryes A, Zhou X, Kuznetsova V, Dempsey F, Pittman K, et al.Triton X-100 (Cat. #BP151-100, Fisher Scientific) and incubated with antibodies against Mer (Cat. #AF591, R&D Systems) and Stat1 (Cat. #9172S, Cell Signaling Technology). Protein-protein interactions were

(10) Inflammatory crosstalk impairs phagocytic receptors and aggravates atherosclerosis in clonal hematopoiesis in miceThe Journal of clinical investigationNovember 12, 2024Liu W, Hardaway BD, Kim E, Pauli J, Wettich JL, Yalcinkaya M, Hsu CC, Xiao T, Reilly MP, Tabas I, et al.ab23914,1:1,000), anti–histone H3 (Cell Signaling Technology, 4499, 1:1,000), anti-MERTK (R&D Systems, AF591, 1:1,000), and β-actin (Cell Signaling Technology, 4970s, 1:5,000) at 4°C overnight and detected using

(11) Genetically Engineered Macrophages Co-Loaded with CD47 Inhibitors Synergistically Reconstruct Efferocytosis and Improve Cardiac Remodeling Post Myocardial Ischemia Reperfusion InjuryAdvanced healthcare materialsJune 1, 2024Tan H, Li W, Pang Z, Weng X, Gao J, Chen J, Wang Q, Li Q, Yang H, Dong Z, et al.transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, USA), and probed with anti‐Mer (AF591, R&D System, USA) and anti‐CCR2 (MA5‐41175, ThermoFisher Scientific, USA). Antigen‐antibody complexes

(12) Rapid unleashing of macrophage efferocytic capacity via transcriptional pause releaseNatureApril 1, 2024Tufan T, Comertpay G, Villani A, Nelson GM, Terekhova M, Kelley S, Zakharov P, Ellison RM, Shpynov O, Raymond M, et al.activating PtdSer receptors: Axl, R&D Systems, AF854, 20 nM final concentration; Mer, R&D Systems, AF591, 20 nM final concentration; IgG isotype control, R&D Systems, AB-108-C, 20 nM final concentration. Opsonization. The

(13) Mer tyrosine kinase regulates bone metabolism, and its deficiency partially ameliorates periodontitis- and ovariectomy-induced bone loss in miceJBMR PlusFebruary 15, 2024Ka-Young Ryu, Nitin Kumar Pokhrel, Hye-Jin Jung, Hyo Jeong Kim, Jiwon Seok, Tae-Young Kim, Hyung Joon Kim, Ji Hye Lee, Jae-Young Kim, Yong-Gun Kim, et al.human/mouse/rat BMP-2 (355-BM) was purchased from R&D Systems (Minneapolis, MN, USA). Anti-mouse MerTK antibody (AF591) was from R&D Systems. Anti-Samd1 (9743), phospho-Smad1/5/8 (9511), β-catenin (9562), GSK3β (12456),

(14) PFKFB2-mediated glycolysis promotes lactate-driven continual efferocytosis by macrophagesNature metabolismMarch 1, 2023Schilperoort M, Ngai D, Katerelos M, Power DA, Tabas I.13064), Akt (CST, no. 4691), phospho-Akt (CST, no. 4060), TXNIP (CST, no. 14715), MerTK (R&D systems, AF591), LRP1 (Abcam, Ab92544), or β-actin (CST, no. 5125; HRP-conjugated), diluted in blocking solution (1:1,000

(15) Walnut inclusion in a palm oil-based atherogenic diet promotes traits predicting stable atheroma plaque in Apoe-deficient miceFrontiers in nutritionJanuary 1, 2023Lázaro I, Bobi J, Cofán M, Kapravelou G, Amor AJ, Surra J, Gómez-Guerrero C, Ortega E, Osada J, Dantas AP, et al.immunohistochemistry with a goat anti-MerTK antibody specific for the ectodomain of mouse MerTK (Cat# AF591, R&D system, Bio-techne, Minneapolis, MN, United States) (15). Concentration used for each antibody

(16) AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate CellsCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Pop OT, Geng A, Flint E, Singanayagam A, Ercan C, Possamai L, Patel VC, Kuenzler P, Meier MA, Soysal S, et al.

(17) Adaptable toolbox to characterize Alzheimer's disease pathology in mouse modelsSTAR protocolsDecember 16, 2022Huang Y, Lemke G.12575182 (lot 4285684) RRID: AB_2572623 "anti-Mer, polyclonal goat (For IF, 1:50)",R&D Systems,"Cat# AF591, Lot DGS0517061, and Lot DGS0213111, RRID: AB_2098565" "anti-mouse Gas6, polyclonal goat (For IF, 1:50–100)",R&D Systems

(18) Anti-inflammatory clearance of amyloid-β by a chimeric Gas6 fusion proteinNature medicineSeptember 1, 2022Jung H, Lee SY, Lim S, Choi HR, Choi Y, Kim M, Kim S, Lee Y, Han KH, Chung WS, et al.

(19) Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunityCell reportsAugust 9, 2022Feriotti C, Sá-Pessoa J, Calderón-González R, Gu L, Morris B, Sugisawa R, Insua JL, Carty M, Dumigan A, Ingram RJ, et al.RRID:AB_2749828 CD335/NKp46,Fluidigm,Cat# 3167008B; RRID:AB_2922922 CD8a,Fluidigm,Cat# 3168003B; RRID:AB_2811241 MerTK,R&D Systems,Cat# AF591; RRID:AB_2098565 CD161/NK1.1,Fluidigm,Cat# 3170002C: RRID:AB_2885023 CD44,Fluidigm,Cat# 3171003C:

(20) Pentoxifylline alleviates ischemic white matter injury through up-regulating Mertk-mediated myelin clearanceJournal of neuroinflammationMay 31, 2022Zheng L, Jia J, Chen Y, Liu R, Cao R, Duan M, Zhang M, Xu Y.anti-MBP (Abcam, ab7349, 1:500), Rabbit anti-Iba-1 (Abcam, ab178847, 1:500), Goat-anti-Mertk (R&D systems, AF591, 1:300), Rabbit anti-PPAR-γ (Abcam, ab45036, 1:500). After incubating with appropriate secondary antibodies

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5/3/2017In Vivo Use Of Mer Antibody Juwen DuBoisWe wanted to determine whether Anti-Mer antibody would inhibit Mer activation and lead to a worse clinical outcome during murine autoimmune diseases.

Mer Antibody

Mer Antibody

Mer Antibody

Mer Antibody

Mer Antibody

Mouse Mer Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (86)

Reviews

Mouse Mer Antibody from R&D Systems, a Bio-Techne Brand

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