GFR alpha-2/GDNF R alpha-2 Antibody AF429 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGFR alpha-2/GDNF R alpha-2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF429
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry
TargetGFR alpha-2/GDNF R alpha-2
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Rat,Transgenic Mouse
IsotypeIgG
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse GFR alpha -2/GDNF R alpha -2, Ser22-Ser441, Accession # Q3UUD8
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameGDNF family receptor alpha 2
NCBI Gene AliasesGDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse GFR alpha -2/GDNF R alpha -2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human GFR alpha -3 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study GFR alpha-2 in mouse models?

Yes, the GFR alpha-2/GDNF R alpha-2 antibody has been shown to effectively detect GFR alpha-2 in mouse models, as evidenced by its use in immunohistochemistry in studies involving peripheral nerve injury and chronic pain models (, ).

What applications has this antibody been utilized in according to the literature?

The GFR alpha-2/GDNF R alpha-2 antibody has been utilized in various applications including Western blotting, immunofluorescence, and immunohistochemistry, as demonstrated in studies examining neuronal signaling and regeneration in response to injury (, ).

Is there any information on the specificity of this antibody in detecting GFR alpha-2?

The antibody has been characterized to detect human and mouse GFR alpha-2 with less than 5% cross-reactivity with recombinant human GFR alpha-3, making it a specific tool for studying GFR alpha-2 in various experimental contexts ().

How has this antibody contributed to understanding chronic pain mechanisms?

The GFR alpha-2/GDNF R alpha-2 antibody has been used to investigate the role of GFR alpha-2 in chronic pain models, revealing its involvement in the signaling pathways that contribute to pain hypersensitivity and neuronal regeneration following injury (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

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(2) Peripheral immune response and axonal degeneration in the hind paw skin of mice with experimental autoimmune encephalomyelitisNeurobiology of PainJanuary 1, 2026Andrea G. Klassen, Timothy N. Friedman, Gustavo Tenorio, Jason R. Plemel, Anna M.W. Taylor, Bradley J. KerrBiotechnology 187–010116, 1:500), CGRP (Millipore Sigma PC205L-100UL, 1:500), and GFRα2 (R&D Systems AF429, 1:200). The following day, slides were washed 2 × 10 min in PBST and 1 × 10 min in PBS prior to secondary

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(4) Maternal stress triggers early-life eczema through fetal mast cell programmingNatureOctober 1, 2025Serhan N, Abdullah NS, Gheziel N, Loste A, Ekren R, Labit E, Gonzalez AA, Oliva G, Tarot P, Petitfils C, et al.1:100), Alexa Fluor 647 anti-Tubulin β3 (BioLegend; 801210; 1:50), anti-human/mouse GFRα2 (R&D Systems; AF429; 1:200), anti-mouse NFH (Sigma; AB1989; 1:1,000), anti-GFP (Proteintech; pabg1; 1:200), anti-RFP (Takara;

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(6) Early life microbiota colonization programs nociceptor sensitivity by regulating NGF production in mast cellsMucosal immunologyApril 1, 2025Abdullah NS, Bradaia A, Defaye M, Ohland C, Svendsen K, Dickemann A, Delanne-Cumenal M, Hassan A, Iftinca M, McCoy KD, et al.

(7) Skin Reinnervation by Collateral Sprouting Following Spared Nerve Injury in MiceJ NeurosciApril 10, 2024Sang-Min Jeon, Aishwarya Pradeep, Dennis Chang, Leah McDonough, Yijia Chen, Alban Latremoliere, LaTasha K. Crawford, Michael J. Caterina(Sicgen, 1:500, #AB0020), rabbit anti CGRP (ImmnuoStar, 1:1000, #24112), Goat anti-GFRα2 (R&D Systems, #AF429), goat anti CGRP (Abcam, 1:500, #AB36001), Biotin-IB4 (Sigma, 1:100, #L2140), sheep anti-TH (EMD Millipore,

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(9) Expression of Glial Cell-Derived Neurotrophic Factor Receptors Within Nucleus Ambiguus During Rat DevelopmentThe LaryngoscopeSeptember 1, 2023Blount Q, Hernandez-Morato I, Moayedi Y, Pitman MJ.RRID:AB_2314683; GFRα-1: (R and D Systems Cat# AF560, RRID:AB_2110307); GFRα-2: (R and D Systems Cat# AF429, RRID:AB_2294621); GFRα-3: (R and D Systems Cat# AF2645, RRID:AB_2110295); Ret: (R and D Systems Cat#

(10) Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heartNPJ Regenerative medicineMarch 3, 2023Siatra P, Vatsellas G, Chatzianastasiou A, Balafas E, Manolakou T, Papapetropoulos A, Agapaki A, Mouchtouri ET, Ruchaya PJ, Korovesi AG, et al.labeled with the following antibodies: anti-human/mouse GFRA2 Polyclonal Goat IgG (R&D Systems, Cat no. AF429), rat monoclonal anti-PDGFR alpha antibody conjugated with PE (Abcam, APA5, Cat no. ab93531), donkey

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(12) A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exerciseCell stem cellApril 7, 2022Gadomski S, Fielding C, García-García A, Korn C, Kapeni C, Ashraf S, Villadiego J, Toro RD, Domingues O, Skepper JN, et al.RRID:AB_1269734), rabbit anti-VIP (Progen, Cat. No. 11428), goat anti-GFRα2 (R&D Systems, Cat. No. AF429; RRID:AB_2294621), rat anti-RUNX2 (BioLegend, Cat. No. 692802; RRID:AB_2632769), goat anti-osteolectin

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(14) Gut-innervating TRPV1+ Neurons Drive Chronic Visceral Pain via Microglial P2Y12 ReceptorCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Defaye M, Abdullah NS, Iftinca M, Hassan A, Agosti F, Zhang Z, Cumenal M, Zamponi GW, Altier C.rabbit anti-CGRP, 1:1000, Sigma-Aldrich, Cat. No.PC205L; goat anti-GFRα2, 1:500, R&D Systems, Cat. No. AF429; anti-IB4-coupled Alexa 594, 1:1000, Invitrogen, Cat. No. I21412; anti-HA, 1:500, Biolegend, Cat. No.

(15) Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specificationNeural developmentSeptember 21, 2021Muzyka VV, Badea TC.information. See also extensive reference list in RRID). GFRα2 1:100 Anti- GFRα2 antibody (R&D Systems; AF429, RRID: AB_2294621) is a polyclonal goat antibody raised against NS0-derived recombinant mouse GFRα-2/GDNF

(16) Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditionsNature metabolismJune 1, 2021Boyd JT, LoCoco PM, Furr AR, Bendele MR, Tram M, Li Q, Chang FM, Colley ME, Samenuk GM, Arris DA, et al.Biolegend), TRPV1 (1:700; GP14100, Neuromics), PLA2g7 (1:500; 15526–1-AP, ProteinTech), and GFRA2 (1:500; AF429, R&D Systems). Isolectin GS-IB4 Alexa Fluor® 488 Conjugate (1:800 from stock; 121411, ThermoFisher)

(17) ARID1a Associates with Lymphoid-Restricted Transcription Factors and Has an Essential Role in T Cell DevelopmentJournal of immunology (Baltimore, Md. : 1950)September 1, 2020Astori A, Tingvall-Gustafsson J, Kuruvilla J, Coyaud E, Laurent EMN, Sunnerhagen M, Åhsberg J, Ungerbäck J, Strid T, Sigvardsson M, et al.

(18) Cutaneous TRPV1(+) Neurons Trigger Protective Innate Type 17 Anticipatory ImmunityCellAugust 8, 2019Cohen JA, Edwards TN, Liu AW, Hirai T, Jones MR, Wu J, Li Y, Zhang S, Ho J, Davis BM, et al.(URA-1),Biolegend,146803 Fixable viability dye eFlour780,Invitrogen,65-0865-14 goat anti-GFRα2,R&D Systems,AF429 goat anti-GFRα3,R&D Systems,AF2645 rabbit anti-PGP9.5,Thermo Fisher,PA121097 rabbit anti-TRPV1,Alomone Labs

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(20) GDNF, Neurturin, and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute to Inflammatory Bone PainThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 23, 2018Nencini S, Ringuet M, Kim DH, Greenhill C, Ivanusic JJ.445) RRID:AB_2110307 1:300 GFR␣2 Mouse myeloma cell line NS0-derived recombinant Goat polyclonal; catalog #AF429, R&D Systems/ RRID:AB_2294621 mouse GFR␣2 (22– 441) 1:300 GFR␣3 Goat polyclonal; catalog #AF2645,

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