Human VEGFR3/Flt-4 Antibody AF349 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman VEGFR3/Flt-4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF349
NCBI Gene AliasesFLT-4, FLT41, CHTD7, VEGFR3, LMPHM1, PCL, LMPH1A, VEGFR-3
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
ReactivityHuman,Mouse,Transgenic Mouse
HostGoat
TargetVEGFR3/Flt-4
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
IsotypeIgG
NCBI Full Gene Namefms related receptor tyrosine kinase 4
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human VEGFR3/Flt-4, Tyr25-Ile776, Accession # P35916
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The development of early human lymphatic vessels as characterized by lymphatic endothelial markersThe EMBO journalMarch 1, 2024Yamaguchi S, Minamide N, Imai H, Ikeda T, Watanabe M, Imanaka-Yoshida K, Maruyama K.(AF2727, R&D Systems, 1:150, RRID:AB_2170716), LYVE1 (ab14917, abcam, 1:150, RRID:AB_301509), VEGFR3 (AF349, R&D Systems, 1:100, RRID:AB_355314), Coup-TF2 (EPR18443, abcam, 1:150, RRID:AB_2895604), D2-40 (anti-PDPN)

(2) Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedemaEMBO molecular medicineFebruary 1, 2024Creff J, Lamaa A, Benuzzi E, Balzan E, Pujol F, Draia-Nicolau T, Nougué M, Verdu L, Morfoisse F, Lacazette E, et al.#9571S), eNOS (Cell Signalling #5880S, 1/10,000), E2F8 (Abcam, AB109596, 1/2000), VEGFR3 (R&D System AF349, 1/200), Phospho-VEGFR3 (Affinity AF3676, 1/1000), CCBE1 (Sigma SAB1402017, 1/100). After three washes

(3) B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancerCancer gene therapyNovember 1, 2023Garg R, Endzhievskaya S, Williamson M.(F1804, Sigma (1:1000)), rabbit polyclonal anti-Myc (C3956, Sigma-Aldrich (1:1000)), goat anti-PlexinB1 (AF349, R&D Systems (1:500)), mouse anti-RanGAP1 (NBP2-0262, Novus Biologicals (1:500)), rabbit anti-Ran (4462,

(4) VEGFR3 is required for button junction formation in lymphatic vesselsCell reportsJuly 25, 2023Jannaway M, Iyer D, Mastrogiacomo DM, Li K, Sung DC, Yang Y, Kahn ML, Scallan JP.Fluor 647 donkey anti-goat IgG (H + L),Invitrogen,A–21447; RRID:AB_141844 goat anti-VEGFR3,R&D Systems,AF349; RRID:AB_355314 rabbit anti-VEGFR2,Cell Signaling,2479; RRID:AB_2212507 rabbit anti-phospho-VEGFR2

(5) Cysteine oxidation of copper transporter CTR1 drives VEGFR2 signalling and angiogenesisNature cell biologyJanuary 1, 2022Das A, Ash D, Fouda AY, Sudhahar V, Kim YM, Hou Y, Hudson FZ, Stansfield BK, Caldwell RB, McMenamin M, et al.

(6) Cellular crosstalk regulates the aqueous humor outflow pathway and provides new targets for glaucoma therapiesNature communicationsOctober 18, 2021Thomson BR, Liu P, Onay T, Du J, Tompson SW, Misener S, Purohit RR, Young TL, Jin J, Quaggin SE.as above, using rat anti-mouse CD31 antibody (BD; #553370; 1:100) and goat anti-VEGFR3 (R&D systems #AF349; 1:100) or goat anti-PROX1 antibodies (R&D systems AF2727; 1:250). Samples were then imaged using a

(7) REDD1 is a determinant of low-dose metronomic doxorubicin-elicited endothelial cell dysfunction through downregulation of VEGFR-2/3 expressionExperimental & molecular medicineOctober 1, 2021Park M, Kim J, Kim T, Kim S, Park W, Ha KS, Cho SH, Won MH, Lee JH, Kwon YG, et al.Sigma-Aldrich, Burlington, MA), anti-REDD1 (#10638-1-AP; Proteintech, Rosement, IL), anti-VEGFR-3 (#AF349; R&D Systems), anti-p-VEGFR-3 (#CY111; Cell Applications, Inc., San Diego, CA), and goat anti-mouse

(8) Expression of Vascular Endothelial Growth Factor-C in the Trabecular Meshwork of Patients with Neovascular Glaucoma and Primary Open-Angle GlaucomaJournal of Clinical MedicineJuly 2, 2021Keitaro Hase, Satoru Kase, Atsuhiro Kanda, Yasuhiro Shinmei, Kousuke Noda, Susumu Ishidaincubated in 5% BSA/PBS for 30 min and then incubated with a primary goat antibody against VEGFR3 (1:50) (AF349) (R&D systems) at 4 °C overnight prior to the exposure to Alexa Fluor 546 anti-goat IgG (1:400) for

(9) Influence of VEGF-A, VEGFR-1-3, and neuropilin 1-2 on progression-free: and overall survival in WHO grade II and III meningioma patientsJournal of molecular histologyApril 1, 2021Bernatz S, Monden D, Gessler F, Radic T, Hattingen E, Senft C, Seifert V, Ronellenfitsch MW, Plate KH, Harter PN, et al.(clone: 55B11; Cell Signalling, Danvers, MA, US), goat IgG anti-human VEGFR-3, dilution 1:500 (clone: AF349; R&D Systems, Minneapolis, MN, US), monoclonal rabbit anti-human NRP-1, dilution 1:100 (clone: ab81321;

(10) The role of antibody expression and their association with bladder cancer recurrence: a single-centre prospective clinical-pilot study in 35 patientsBMC urologyNovember 25, 2020Ella-Tongwiis P, Lamb RM, Makanga A, Shergill I, Hughes SF.UK), monoclonal anti-thrombomodulin (clone: 1009; Cell marque UK) and monoclonal anti-VEGFR-3 (clone: AF349; R&D systems UK) were used in this study. Antibodies were stored and treated according to manufacturer’s

(11) Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signalingCell death & diseaseMay 4, 2020Wei ST, Huang YC, Hsieh ML, Lin YJ, Shyu WC, Chen HC, Hsieh CH.VEGFR1 (AF321, R&D Systems, 1:1000 dilution), VEGFR2 (AF357, R&D Systems, 1:1000 dilution), VEGFR3 (AF349, R&D Systems, 1:1000 dilution), PLC-γ1 (#2822, Cell Signaling, 1:1000 dilution), phospho-PLC-γ1 (#2821,

(12) Identification of ILK as a critical regulator of VEGFR3 signalling and lymphatic vascular growthThe EMBO journalJanuary 15, 2019Urner S, Planas-Paz L, Hilger LS, Henning C, Branopolski A, Kelly-Goss M, Stanczuk L, Pitter B, Montanez E, Peirce SM, et al.MAB1997). The following antibodies were used for in vitro PLA studies: goat anti‐human VEGFR3 (R&D Systems, AF349) and mouse anti‐human β1 integrin (Millipore, MAB1987). Co‐stainings were performed using rabbit anti‐mouse

(13) VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 SignalingCirculation researchApril 28, 2017Heinolainen K, Karaman S, D'Amico G, Tammela T, Sormunen R, Eklund L, Alitalo K, Zarkada G.1:1000; Cell Signaling), goat anti-human VEGFR2 (AF357, 1:500; R&D Systems), goat anti-human VEGFR3 (AF349, 1:1000; R&D Systems), or mouse anti-human VEGFR3 (9D9,25 1:1000)–specific primary antibodies. The blots

(14) Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarctionEuropean journal of pharmacologyFebruary 5, 2017Parry TJ, Ganguly A, Troy EL, Luis Guerrero J, Iaci JF, Srinivas M, Vecchione AM, Button DC, Hackett CS, Zolty R, et al.

(15) Carbohydrate-binding protein CLEC14A regulates VEGFR-2- and VEGFR-3-dependent signals during angiogenesis and lymphangiogenesisThe Journal of clinical investigationFebruary 1, 2017Lee S, Rho SS, Park H, Park JA, Kim J, Lee IK, Koh GY, Mochizuki N, Kim YM, Kwon YG.(Cell Signaling Technology; catalog 2479); p–VEGFR-3 (Cell Applications Inc.; catalog CY1115); VEGFR-3 (R&D Systems, catalogs AF349 for human and AF743 for mouse; Santa Cruz Biotechnology Inc., catalog sc321); collagen type IV (Cosmo Bio

(16) Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4PloS oneJanuary 1, 2017Volk-Draper LD, Hall KL, Wilber AC, Ran S.

(17) Specific niches for lung-resident memory CD8+ T cells at the site of tissue regeneration enable CD69-independent maintenanceThe Journal of experimental medicineDecember 12, 2016Takamura S, Yagi H, Hakata Y, Motozono C, McMaster SR, Masumoto T, Fujisawa M, Chikaishi T, Komeda J, Itoh J, et al.

(18) TGF-β1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)PloS oneJanuary 1, 2016Scherer SD, Bauer J, Schmaus A, Neumaier C, Herskind C, Veldwijk MR, Wenz F, Sleeman JP.(R&D Systems, Cat. No. AF2089), 0.2 μg/ml polyclonal goat-anti-human VEGF-R3 (R&D Systems, Cat. No. AF349) and 0.1 μg/ml polyclonal goat-anti-human vimentin (R&D Systems, Cat. No. AF2105) antibodies. For evaluation

(19) Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complexThe Journal of General PhysiologyApril 1, 2015Brian G. Coon, Nicolas Baeyens, Jinah Han, Madhusudhan Budatha, Tyler D. Ross, Jennifer S. Fang, Sanguk Yun, Jeon-Leon Thomas, Martin A. Schwartzphospho-VEGFRs were detected with rabbit anti-VEGFR2 (#2479; Cell Signaling Technology), goat anti-HsVEGFR3 (AF349; R&D Systems), goat anti-MmVEGFR3 (AF743; R&D Systems), rabbit anti-VEGFR2pY1175 (#2478; Cell Signaling Technology),

(20) Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complexThe Journal of cell biologyMarch 30, 2015Coon BG, Baeyens N, Han J, Budatha M, Ross TD, Fang JS, Yun S, Thomas JL, Schwartz MA.phospho-VEGFRs were detected with rabbit anti-VEGFR2 (#2479; Cell Signaling Technology), goat anti-HsVEGFR3 (AF349; R&D Systems), goat anti-MmVEGFR3 (AF743; R&D Systems), rabbit anti-VEGFR2pY1175 (#2478; Cell Signaling Technology

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VEGFR3/Flt-4 Antibody

VEGFR3/Flt-4 Antibody

Human VEGFR3/Flt-4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (33)

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Human VEGFR3/Flt-4 Antibody from R&D Systems, a Bio-Techne Brand

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