Mouse CD4 Antibody AF554 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse CD4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF554
Quantity100 ug (also 25 ug)
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Frozen
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ConcentrationLYOPH
PurityAntigen Affinity-purified
ReactivityMouse,Transgenic Mouse
IsotypeIgG
TargetCD4
HostGoat
Antibody TypePrimary Antibodies
NCBI Gene AliasesL3T4, Ly-4
NCBI Full Gene NameCD4 antigen
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CD4, Lys27-Thr394, Accession # P06332
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) PET-based immunomapping of intratumoral CD4(+) cells to monitor acquired resistance to checkpoint inhibitorsScience advancesJune 27, 2025Pezzana S, Blaess S, Traenkle B, Schaefer A, Ruoff L, Tako B, Castaneda Vega S, Kaiser PD, Wagner T, Gonzalez-Menendez I, et al.

(2) Inhibition of Th2 Differentiation Accelerates Chronic Wound Healing by Facilitating LymphangiogenesisBiomedicinesApril 24, 2025Bracha L. Pollack, Jeremy S. Torrisi, Geoffrey E. Hespe, Gopika Ashokan, Jinyeon Shin, Babak J. Mehrara, Raghu P. Kataruanti-lymphatic vessel endothelial hyaluronan receptor (LYVE)-1 (ab14917; Abcam, Cambridge, MA, USA), anti-CD4 (AF554; R&D Systems, Minneapolis, MN, USA), and anti-Gata3 (sc-22206; Santa Cruz Biotechnology). Secondary

(3) Soluble RAGE Prevents Type 1 Diabetes Expanding Functional Regulatory T CellsDiabetesSeptember 1, 2022Leung SS, Borg DJ, McCarthy DA, Boursalian TE, Cracraft J, Zhuang A, Fotheringham AK, Flemming N, Watkins T, Miles JJ, et al.blocking with 10% donkey serum, and incubation with rabbit anti-CD3 (SP7; Abcam), goat anti-CD4 (no. AF554; R&D Systems), and biotinylated anti-FoxP3 (FJK-16s; eBioscience) antibodies overnight at 4°C. This

(4) LDH-A-Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth ResponsesCancersMay 6, 2022Shindo M, Maeda M, Myat K, Mane MM, Cohen IJ, Vemuri K, Albeg AS, Serganova I, Blasberg R.stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako). Quantification of morphological characteristics

(5) Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structureiScienceNovember 19, 2021Fotheringham AK, Solon-Biet SM, Bielefeldt-Ohmann H, McCarthy DA, McMahon AC, Ruohonen K, Li I, Sullivan MA, Whiddett RO, Borg DJ, et al.#ab16669, clone SP7 -, Abcam, Cambridge, The United Kingdom), anti-CD4 goat polyclonal IgG (10 μg/ml, #AF554 R&D Systems, Minneapolis, USA) and anti-FoxP3 biotinylated rat monoclonal IgG (5 μg/ml, # 13-5773-82,

(6) Selective Inhibition of HDAC3 Targets Synthetic Vulnerabilities and Activates Immune Surveillance in LymphomaCancer discoveryMarch 1, 2020Mondello P, Tadros S, Teater M, Fontan L, Chang AY, Jain N, Yang H, Singh S, Ying HY, Chu CS, et al.Memorial Sloan Kettering Cancer Center. Sections were stained with either anti- CD4 (R&D Systems, cat#AF554, 2ug/ml) or anti-PD-L1 (R&D Systems cat#AF1019, 2ug/ml) or B220 (BD Biosciences, cat# 550286, 0.3ug/ml)

(7) The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune SuppressionCancer cellAugust 12, 2019Su W, Han HH, Wang Y, Zhang B, Zhou B, Cheng Y, Rumandla A, Gurrapu S, Chakraborty G, Su J, et al.550286, 0.3 μg/ml); anti-FoxP3 (eBioscience, cat# 14–577382, 5 μg/ml); anti-CD4 (R&D Systems, cat# AF554, 2 μg/ml); anti-NKp46 (R&D Systems, cat#AF2225, 2 μg/ml) for 5 hours, followed by 60 minutes incubation

(8) Non-conventional Inhibitory CD4⁺Foxp3^-PD-1^hi T Cells as a Biomarker of Immune Checkpoint Blockade ActivityCancer cellJune 11, 2018Zappasodi R, Budhu S, Hellmann MD, Postow MA, Senbabaoglu Y, Manne S, Gasmi B, Liu C, Zhong H, Li Y, et al.anti-mouse IL-21 (clone FFA21),eBioscience,Cat# 17-7211-82 Purified anti-mouse CD4,R&D Systems,Cat# AF554 Purified anti-mouse Foxp3 (clone FJK-16s),eBioscience,Cat# 14-5773-82 Purified anti-mouse PD-1,Sino Biological

(9) Diphtheria toxin-mediated ablation of lymphatic endothelial cells results in progressive lymphedemaJCI insightSeptember 22, 2016Gardenier JC, Hespe GE, Kataru RP, Savetsky IL, Torrisi JS, Nores GD, Dayan JJ, Chang D, Zampell J, Martínez-Corral I, et al.and insight.jci.org doi:10.1172/jci.insight.84095 11 RESEARCH ARTICLE CD4 (catalog AF2125, MAB114, and AF554, respectively; R&D Systems), α-SMA (catalog C6198; Sigma-Aldrich), CD11b (catalog 557395; BD Biosciences),

(10) Determinants in the β and δ subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptorThe Journal of biological chemistryJanuary 3, 2014Rudell JC, Borges LS, Rudell JB, Beck KA, Ferns MJ.

(11) Timing of estrogen replacement influences atherosclerosis progression and plaque leukocyte populations in ApoE-/- miceAtherosclerosisNovember 1, 2008Cann JA, Register TC, Adams MR, St Clair RW, Espeland MA, Williams JK.

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Mouse CD4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (11)

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Mouse CD4 Antibody from R&D Systems, a Bio-Techne Brand

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