Mouse Leptin R Antibody AF497 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Leptin R Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF497
Quantity100 ug (also 25 ug)
ApplicationsCyTof,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ConcentrationLYOPH
PurityAntigen Affinity-purified
HostGoat
ReactivityHuman,Mouse,Transgenic Mouse
TargetLeptin R
IsotypeIgG
Antibody TypePrimary Antibodies
NCBI Gene Aliasesobl, Leprb, db, OB-RGRP, Modb1, LEPROT, obese-like, Obr, diabetes
NCBI Full Gene Nameleptin receptor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Leptin R, Ala20-Gly839, Accession # Q3US58
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What applications has the Leptin R antibody been used for in scientific research?

The Leptin R antibody has been shown to work in various applications including Western blotting, flow cytometry, immunocytochemistry, immunohistochemistry, and immunoprecipitation. For instance, it was utilized in immunofluorescence imaging to assess leptin receptor expression in bone marrow tissues ().

How does the Leptin R antibody perform in terms of species reactivity?

The Leptin R antibody is reactive with mouse and human leptin receptors. It has approximately 30% cross-reactivity with recombinant human leptin receptor in direct ELISAs, making it suitable for studies involving both species ().

Can you provide an example of how the Leptin R antibody has been applied in a specific study?

In a study investigating the role of leptin receptors in hematopoietic stem cell niches, the Leptin R antibody was used to stain tissues for immunofluorescence, allowing researchers to visualize the expression of leptin receptors in perivascular cells of the bone marrow ().

What is the recommended dilution for using the Leptin R antibody in immunofluorescence?

The Leptin R antibody is recommended to be used at a dilution of 1:100 for immunofluorescence applications, as indicated in various studies that employed this antibody for detecting leptin receptor expression ().

R&D Systems, a Bio-Techne Brand

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Company Profile

Website: http://www.rndsystems.com

(1) GREM1 acts in leptin receptor-expressing skeletal cells to mediate peri-implant fibrosisNature CommunicationsMarch 23, 2026Vincentius Jeremy Suhardi, Anastasia Oktarina, Yingzhen Niu, Andrew L. Thomson, Jϋrgen Alphonsus, Nicolas Suhardi, Jason McCormick, Ugur Ayturk, Matthew B. Greenblatt, Lionel B. Ivashkiv, et al.antibodies for IHC Primary antibodies used for immunofluorescence included murine LEPR (R&D System, AF497, 1:100 dilution), human LEPR (R&D system, MAB867), GREM1 (LSBio, LS-C125371, 1:100 dilution), murine

(2) Bone marrow endosteum houses Hedgehog-susceptible Dlx5-expressing osteoblast precursor cellsCommunications BiologyFebruary 27, 2026Keita Kondo, Yuki Matsushita, Shion Orikasa, Yuta Nakai, Aiko Kuroda, Shinsuke Ohba, Wanida Ono, Noriaki Onopolyclonal antibody (1:200, R&D systems, AF2910), goat anti-LepR polyclonal antibody (1:200, R&D systems, AF497), and rabbit anti-Cathepsin K monoclonal antibody (1:200, Abcam, ab300569) overnight at 4 °C, and subsequently

(3) Female Infertility and Risk for Later-Life Cardiovascular Disease: Lessons from a Mouse Model of Human Cardiovascular DiseaseReproductive SciencesJanuary 16, 2026Ayaka Tanaka, Hitomi Nakamura, Namhyo Kim, Hajime Nakaoka, Makoto Nishida, Keiichi Kumasawa, Yasushi Sakata, Shizuya Yamashita, Tadashi KimuraAviva Systems biology, CA, US) at 1:400 dilution, polyclonal goat anti-mouse leptin receptor antibody (AF497; R&D Systems, MN, US) at 1:200 dilution, and polyclonal rabbit anti-mouse cyclooxygenase (COX) −2 antibody

(4) Senescent Cells Involved in Deterioration of Bone Microstructure by High-Frequency Parathyroid Hormone 1-34 Administration and Bone LossAging CellDecember 23, 2025Masayuki Bun, Yuichiro Ukon, Masato Ikuta, Takayuki Kitahara, Takuya Furuichi, Hiromasa Hirai, Daisuke Tateiwa, Yuya Kanie, Masayuki Furuya, Takahito Fujimori, et al.Cell Signaling Technology), rabbit monoclonal anti‐phospho‐4EBP1 (1:800), mouse Leptin R antibody (AF497, 1:250, R&D Systems), mouse Dentin Matrix Protein 1 (DMP‐1) antibody (AF4386, 1:250, R&D Systems), Runt‐related

(5) IPSC-derived organoid-sourced skin cells enable functional 3D skin modeling of recessive dystrophic epidermolysis bullosaJ Tissue EngDecember 1, 2025Laura Garriga-Cerda, Alberto Pappalardo, Charlotte Y. Lee, Jeffrey Kysar, Kristin Myers, Hasan Erbil Abaciused were: collagen VII (EMD Millipore #234192), keratin 14 (BioLegend #906004), LEPr (R&D Systems #AF497), LEF1(Santa Cruz #sc-374412), CD90 (BioLegend #328110). All samples were incubated with the corresponding

(6) A Novel Therapeutic Strategy for Bone Marrow Failure: Niche Rejuvenation Using Costal Cartilage‐Derived Stem CellsAdvanced ScienceNovember 1, 2025Rui Dong, Zhiguo Ling, Pengyuan Fan, Debao Li, Jinsong Wang, Wenjiong Shi, Rui Zuo, Runfeng Chen, Xuemin Sun, Lang Xiao, et al.USA), CD31 (390, BioLegend, USA), F4/80 (BM8, BioLegend, USA), CD11b (M1/70, BioLegend, USA), Lepr (AF497, R&D Systems, France), and Apoptosis Detection Kit (88‐8006‐72, eBioscience, USA). Flow cytometry assays

(7) Megakaryocytes transfer mitochondria to bone marrow mesenchymal stromal cells to lower platelet activationJournal of Clinical InvestigationApril 15, 2025Chengjie Gao, Yitian Dai, Paul A. Spezza, Paul Boasiako, Alice Tang, Giselle Rasquinha, Hui zhong, Bojing Shao, Yunfeng Liu, Patricia A. Shi, et al.Triton X-100. Tissues were stained with CD41 (Biolegend, catalog 133908) and Lepr (R&D Systems, catalog AF497) and subsequent secondary antibody staining of goat anti-donkey Alexa Fluor 568 (Fisher, catalog A-11057).

(8) Megakaryocytes transfer mitochondria to bone marrow mesenchymal stromal cells to lower platelet activationJ Clin InvestFebruary 27, 2025Chengjie Gao, Yitian Dai, Paul A. Spezza, Paul Boasiako, Alice Tang, Giselle Rasquinha, Hui Zhong, Bojing Shao, Yunfeng Liu, Patricia A. Shi, et al.Triton X-100. Tissues were stained with CD41 (Biolegend, catalog 133908) and Lepr (R&D Systems, catalog AF497) and subsequent secondary antibody staining of goat anti-donkey Alexa Fluor 568 (Fisher, catalog A-11057).

(9) Western diet promotes endometriotic lesion growth in mice and induces depletion of Akkermansia muciniphila in intestinal microbiotaBMC MedicineNovember 6, 2024Guillaume Parpex, Benoît Chassaing, Mathilde Bourdon, Pietro Santulli, Ludivine Doridot, Marine Thomas, Frédéric Batteux, Sandrine Chouzenoux, Charles Chapron, Carole Nicco, et al.anti-Ki67 antibody (ab15580, Abcam) at a 1/500 dilution and with an leptin (Ob) receptor antibody (af497, R&D Systems) at a 1/100 dilution. After washing, the secondary antibody HRP conjugate was applied,

(10) A Murine Model of Non-Wear-Particle-Induced Aseptic LooseningBiomimetics (Basel)November 4, 2024Vincentius Suhardi, Anastasia Oktarina, Yingzhen Niu, Branden Sosa, Julia Retzky, Matthew Greenblatt, Lionel Ivashkiv, Mathias Bostrom, Xu Yang(711765, Invitrogen, NY, USA); anti-Pu.1 (22585, Cell Signaling, Danvers, MA, USA); anti-Leptin receptor (AF497, R&D Systems, Minneapolis, MN, USA); Anti-Endomucin (sc65495, Santa Cruz Biotech, Santa Cruz, CA, USA);

(11) Uptake of ox-LDL by binding to LRP6 mediates oxidative stress-induced BMSCs senescence promoting obesity-related bone lossCellular signallingMay 1, 2024Meng S, Wang Z, Liu X, Shen K, Gu Y, Yu B, Wang L.

(12) Mdm2/p53 levels in bone marrow mesenchymal stromal cells are essential for maintaining the hematopoietic niche in response to DNA damageCell death & diseaseJune 23, 2023Pourebrahim R, Heinz Montoya R, Alaniz Z, Ostermann L, Lin PP, Liu B, Ayoub E, Burks JK, Andreeff M.primary antibody incubation against perilipin (Cell Signaling 9349 S), leptin receptor (R&D Systems AF497), and p53 (CM5, Leica P53-CM5P-L) was performed at antibody-specific dilutions in a light-protected

(13) Mdm2/p53 levels in bone marrow mesenchymal stromal cells is essential for maintaining the hematopoietic niche in response to DNA damageResearch squareMarch 2, 2023Pourebrahim R, Montoya RH, Alaniz Z, Ostermann L, Lin PP, Liu B, Ayoub E, Burks JK, Andreeff M.primary antibody incubation against perilipin (Cell Signaling 9349S), leptin receptor (R&D Systems AF497), and p53 (CM5, Leica P53-CM5P-L) was performed at antibody-specific dilutions in a light-protected

(14) Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in MiceNutrientsFebruary 21, 2023Decourtye-Espiard L, Clemessy M, Leneuve P, Mire E, Ledent T, Le Bouc Y, Kappeler L.antibody,"Proteogenix, VC-15","Rabbit, polyclonal",1/500 Leptin R,Goat anti-mouse leptin receptor,"R&D system, AF497",Goat polyclonal,1/250 Neurofilament,Anti-160 kD Neurofilament Medium antibody,"Abcam, ab72998","Chicken,

(15) Bone Metastasis Initiation Is Coupled with Bone Remodeling through Osteogenic Differentiation of NG2+ CellsCancer discoveryFebruary 6, 2023Zhang W, Xu Z, Hao X, He T, Li J, Shen Y, Liu K, Gao Y, Liu J, Edwards DG, et al.(EMD Millipore, Cat# AB5320, RRID: AB_11213678, 1mg/ml, 1:200); Goat anti-mouse Leptin Receptor (R&D system, Cat# AF497, RRID: AB_2281270, 1mg/ml, 1:100); Rabbit anti-mouse PDGFRβ (Abcam, Cat# ab32570, RRID: AB_7771650.162mg/ml,

(16) Multiparametric senescent cell phenotyping reveals CD24 osteolineage cells as targets of senolytic therapy in the aged murine skeletonbioRxiv : the preprint server for biologyJanuary 13, 2023Doolittle ML, Saul D, Kaur J, Rowsey JL, Vos SJ, Pavelko KD, Farr JN, Monroe DG, Khosla S.

(17) Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageingNature cell biologyJanuary 1, 2023Mitchell CA, Verovskaya EV, Calero-Nieto FJ, Olson OC, Swann JW, Wang X, Hérault A, Dellorusso PV, Zhang SY, Svendsen AF, et al.and washes 3x with PBS. For LepR staining, sections were first stained with LepR-biotin (R&D systems, AF497) and rabbit anti-mouse laminin, and then with SA-A555 (Thermo Fisher, S21381) and goat anti-rabbit A594

(18) Ras drives malignancy through stem cell crosstalk with the microenvironmentNatureDecember 1, 2022Yuan S, Stewart KS, Yang Y, Abdusselamoglu MD, Parigi SM, Feinberg TY, Tumaneng K, Yang H, Levorse JM, Polak L, et al.

(19) Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repairCell stem cellNovember 3, 2022Jeffery EC, Mann TLA, Pool JA, Zhao Z, Morrison SJ.

(20) Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissuesSTAR protocolsSeptember 16, 2022Saçma M, Matteini F, Mulaw MA, Hageb A, Bogeska R, Sakk V, Vollmer A, Marka G, Soller K, Milsom MD, et al.(1:100),Jackson ImmunoResearch,Cat# 705-605-003; RRID: AB_2340436 anti-LepR (1:100),R&D Systems,Cat# AF497; RID: AB_2281270 anti-goat Cy3-conjugated (1:100),Jackson ImmunoResearch,Cat# 705-165-003; RRID: AB_2340411 anti-H4K16ac

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Leptin R Antibody

Leptin R Antibody

Mouse Leptin R Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (56)

Reviews

Mouse Leptin R Antibody from R&D Systems, a Bio-Techne Brand

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