Oct-3/4 Antibody AF1759 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemOct-3/4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1759
NCBI Gene AliasesOTF-3, OCT4, OCT3, Oct3/4, OTF3, Oct-3, Oct-4, OTF4
ApplicationsBioassay,Co-Immunoprecipitation,Differentiation,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Simple Western,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetOct-3/4
IsotypeIgG
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Primate - Macaca mulatta (Rhesus Macaque),Primate - Pongo (Orangutan),Rabbit,Rat,Transgenic Mouse,Xenograft
HostGoat
NCBI Full Gene NamePOU class 5 homeobox 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Oct-3/4, Met1-Asn265 (Met262Leu), Accession # Q01860
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Oct-3/4 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunofluorescence applications in stem cell research?

Yes, the Oct-3/4 antibody has been successfully used in immunofluorescence assays, as demonstrated in studies where it was employed to detect pluripotency markers in induced pluripotent stem cells and human fibroblasts (, ).

What species does this antibody react with, and how has it been utilized in research?

The Oct-3/4 antibody is reactive with human, mouse, rabbit, rat, and non-human primate species. It has been utilized in various applications, including Western blotting and immunocytochemistry, to study pluripotency and differentiation in stem cells (, ).

Is there any evidence of this antibody being used in studies related to cancer stem cells?

Yes, the Oct-3/4 antibody has been referenced in studies investigating retinoblastoma, where it was used to identify stem cell-like properties in tumor cells, indicating its relevance in cancer stem cell research ().

How does this antibody contribute to the understanding of pluripotency in stem cells?

The Oct-3/4 antibody is crucial for detecting the expression of the Oct-3/4 protein, a key marker of pluripotency. Its application in various studies has helped elucidate the mechanisms of pluripotency and differentiation in stem cells, as seen in research focusing on induced pluripotent stem cells and embryonic stem cells (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pharmacological Inhibition of SP1 Reverses Cancer Stemness and Enhances Sorafenib Efficacy in Hepatocellular CarcinomaCellsMay 22, 2026Maël Padelli, Christophe Desterke, Aurore Devocelle, Denis Clay, Agnès Bourillon, Georges Uzan, Antoinette Lemoine, Julien Giron-MichelSOX2 (Bio-Techne, Minneapolis, MN, USA, #MAB2018, 1/500), Oct4 (Bio-Techne, Minneapolis, MN, USA, #AF1759, 1/1000), Nanog (Santa Cruz Biotechnology, Dallas, TX, USA, #sc-134218, 1/200), CD133 (Miltenyi Biotec,

(2) Biomaterial-minimalistic photoactivated bioprinting of cell-dense tissuesCellJanuary 8, 2026Mian Wang, Wanlu Li, Jin Hao, Ling Cai, Xuan Mei, Regina Sanchez Sanchez Flores, Penélope Cerón Cerón Castillo, Carlos Ezio Garciamendez-Mijares, Xuan Mu, Xiao Kuang, et al.anti-SOX-2,R&D Systems,Cat# MAB2018; RRID AB_358009 Goat monoclonal anti-Oct-3/4,R&D Systems,Cat# AF1759; RRID: AB_354975 Rabbit monoclonal anti-Albumin,Abcam,Cat# ab207327; RRID: AB_2755031 F-actin monoclonal

(3) The epigenetic regulator SETDB1 as a key component of cancer stem cells and drug resistance in primary liver cancerCell Oncol (Dordr)January 6, 2026Maël Padelli, Christophe Desterke, Aurore Devocelle, Georges Uzan, Antoinette Lemoine, Julien Giron-Michelprimary antibodies: SETDB1 (ab-107225, Abcam, 1:750), HIF1A (cat.610359, BD Biosciences, 1:1000), OCT4 (AF1759, Biotechne, 1/1000), NANOG (sc-134218, SantaCruz Biotechnology, 1/200), CD133 (130-092-395, Miltenyi Biotec

(4) Molecular and epistatic interactions between pioneer transcription factors shape nucleosome dynamics and cell differentiationNature CommunicationsDecember 10, 2025Rémi-Xavier Coux, Agnès Dubois, Almira Chervova, Inma Gonzalez, Sandrine Vandormael-Pournin, Michel Cohen-Tannoudji, Pablo Navarro#14-1401-82), Rabbit Monoclonal anti-SOX17 (Abcam #ab224637), goat polyclonal anti-OCT4 (R&D systems #AF1759), goat polyclonal anti GATA4 (Santa Cruz #sc1237), goat polyclonal anti-OCT4 (Santa Cruz N19 #sc8628),

(5) Generation of a human iPSC line with EGFP knock-in at the ATRX locus for visualization of chromatin remodeler dynamics and nuclear condensate formationStem cell researchDecember 1, 2025Tomooka R, Okano H, Kohyama J.

(6) Stage-sensitive potential of isolated rabbit ICM to differentiate into extraembryonic lineages†Biology of ReproductionNovember 14, 2025Katarzyna Filimonow, Anna Chołoniewska, Jan Chołoniewski, Zofia E Madeja, Katarzyna Barłowska, Joanna Grabarek, Elżbieta Wenta-Muchalska, Berenika Plusa, Anna PiliszekNumber,RRID CDX2,Mouse,BioGenex,MU392A-UC,AB_2923402 GATA3,Mouse,BioLegend,653801,AB_2561776 GATA3,Mouse,BioLegend,653809,AB_2563217 GATA3,Goat,R&D systems,AF2605,AB_2108571 OCT4,Goat,R&D systems,AF1759,AB_354975 SOX2,Rabbit,Abcam,ab97959,AB_2341193 SOX2,Goat,R&D systems,AF2018,AB_355110 SOX17,Goat,R&D systems

(7) The NuRD component CHD3 promotes BMP signalling during cranial neural crest cell specificationEMBO ReportsAugust 20, 2025Zoe H Mitchell, Joery den Hoed, Willemijn Claassen, Martina Demurtas, Laura Deelen, Philippe M Campeau, Karen Liu, Simon E Fisher, Marco TrizzinoActive Motif 39843), anti-NANOG (goat, 1:50, Bio-techne AF1997), anti-OCT4 (goat, 1:50, Bio-techne AF1759), anti-TFAP2A (mouse, 1:100, Fisher Scientific 11594723), anti-CHD3 (rabbit, 1:500, Bethyl A301-220A),

(8) deepBlastoid: A Deep Learning Model for Automated and Efficient Evaluation of Human BlastoidsLife MedicineJuly 11, 2025Zejun Fan, Zhenyu Li, Yiqing Jin, Arun Pandian Chandrasekaran, Ismail M Shakir, Yingzi Zhang, Aisha Siddique, Mengge Wang, Xuan Zhou, Yeteng Tian, et al.primary antibodies used included SOX2 (Cell Signaling Technology, MAB4900S, 1:50), OCT4 (R&D Systems, AF1759), GATA3 (R&D Systems, MAB6300), and GATA4 (Cell Signaling Technology, MAB36966S). After primary antibody

(9) Physiological role and mechanisms of action for a long noncoding haplotype regionCell ReportsJune 24, 2025Hong Xue, Manoj K. Mishra, Yong Liu, Pengyuan Liu, Michael Grzybowski, Rajan Pandey, Kristie Usa, Mark A. Vanden Avond, Niharika Bala, Abdel A. Alli, et al.incubation with relevant antibodies for 1 h at room temperature. For iPSCs characterization, OCT-4 (1:200, AF1759, R&D Systems), NANOG (1:200, 09–0020, Reprocell), TRA-1 (1:100, sc-21706, Santa Cruz Biotechnology)

(10) Changes in metabolite profiles in the cerebrospinal fluid and in human neuronal cells upon tick-borne encephalitis virus infectionJ NeuroinflammationJune 14, 2025Satoshi Suyama, Sally Boxall, Benjamin Grace, Andrea Fořtová, Martina Pychova, Lenka Krbkova, Rupasri Mandal, David Wishart, Diane E. Griffin, Daniel Růžek, et al.1. Immunofluorescence assays The expression of Nanog (R&D Systems; AF1997), Oct-3/4 (R&D Systems; AF1759), beta-III Tubulin (TuJ-1) (Abcam; ab18207), HB9 (Stratech; ORB157435-BOR) and TBEV envelope (E) glycoprotein

(11) Self-organization of mouse embryonic stem cells into reproducible pre-gastrulation embryo models via CRISPRa programmingCell Stem CellJune 5, 2025Gerrald A. Lodewijk, Sayaka Kozuki, Clara J. Han, Benjamin R. Topacio, Seungho Lee, Lily Nixon, Abolfazl Zargari, Gavin Knight, Randolph Ashton, Lei S. Qi, et al.polyclonal anti-Sox17,R&D Systems,Cat#AF1924; RRID: AB_355060 Goat polyclonal anti-Oct3/4,R&D Systems,Cat#AF1759; RRID: AB_354975 Mouse monoclonal anti-Oct3/4,Santa Cruz Biotechnology,Cat#sc-5279; RRID: AB_628051 Rabbit

(12) Drug and siRNA screens identify ROCK2 as a therapeutic target for ciliopathiesCommun Med (Lond)April 19, 2025Claire E. L. Smith, Andrew J. Streets, Alice V. R. Lake, Subaashini Natarajan, Sunayna K. Best, Katarzyna Szymanska, Magdalena Karwatka, Thomas Stevenson, Rachel Trowbridge, Gary Grant, et al.modification (GT335, Adipogen, AG-20B-0020-C100), anti g-tubulin (Abcam Ab137822), anti-OCT3/4 (R&D Systems AF1759) and anti-SSEA4 (BD Biosciences 560218) preconjugated with Alexa Fluor 555, anti-RPGRIP1L (Proteintech

(13) Optimization of Transcription Factor-Driven Neuronal Differentiation from Human Induced Pluripotent Stem Cells for Disease Modelling and Drug ScreeningStem cell reviews and reportsApril 1, 2025Servetti M, Caramia M, Parodi G, Loiacono F, Nano E, Biddau G, Ferrando L, Morinelli L, Valente P, Martinoia S, et al.were used: SOX2 (Millipore; AB5603; 1:500), NANOG (R&D Systems; AF1997; 1:500), OCT3/4 (R&D Systems; AF1759; 1:40), and SSEA-4 (Abcam; 16,287; 1:200). Secondary antibodies were used at a dilution of 1:500. Synaptic

(14) Generation of human induced pluripotent stem cell lines derived from patients of cystic biliary atresiaHuman CellNovember 13, 2024Ningxin Ge, Kan Suzuki, Iori Sato, Michiya Noguchi, Yukio Nakamura, Mami Matsuo-Takasaki, Jun Fujishiro, Yohei Hayashi/western blot Antibody,Dilution,Company cat #,RRID Pluripotency marker,Goat anti-OCT3/4,1:200,"R and D Systems, Cat# AF1759",RRID:AB_354975 Pluripotency marker,Rabbit anti-NANOG,1:500,"ReproCELL Incorporated, Cat# RCAB004P-F,",RRID:AB_1560380 Pluripotency

(15) Physioxia rewires mitochondrial complex composition to protect stem cell viabilityRedox biologyNovember 1, 2024Raabe J, Wittig I, Laurette P, Stathopoulou K, Brand T, Schulze T, Klampe B, Orthey E, Cabrera-Orefice A, Meisterknecht J, et al.

(16) Glycogen myophosphorylase loss causes increased dependence on glucose in iPSC-derived retinal pigment epitheliumThe Journal of biological chemistryAugust 1, 2024Basu B, Karwatka M, China B, McKibbin M, Khan K, Inglehearn CF, Ladbury JE, Johnson CA.were fixed with 4% paraformaldehyde for 15 min before staining for antibodies. The Oct3/4 antibody (AF1759, R&D Systems, Inc) was used at 10 μg/ml and the preconjugated SSEA4 Alexa Fluor 555 antibody (560218,

(17) Generation of an Alagille Syndrome (ALGS) patient-derived induced pluripotent stem cell line (TRNDi036-A) carrying a heterozygous mutation (p.Cys693*) in the JAG1 geneStem Cell ResearchJune 1, 2024Omer Hatim, Miao Xu, Ivan Pavlinov, Kaari Linask, Jeanette Beers, Jizhong Zou, Chengyu Liu, Steven Rodems, Karsten Baumgärtel, Melissa A. Gilbert, et al."R & D systems, Cat# MAB2018",RRID: AB_358009 Pluripotency Markers,Goat anti-OCT3/4,1:200,"R & D, Cat# AF1759",RRID: AB_354975 Pluripotency Markers,Mouse anti-TRA-1–60,1:200,"Cell signaling, Cat# 4746",RRID: AB_2119059 Pluripotency

(18) Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxivMarch 10, 2024Gerrald A. Lodewijk, Sayaka Kozuki, Clara Han, Benjamin R. Topacio, Abolfazl Zargari, Seungho Lee, Gavin Knight, Randolph Ashton, Lei S. Qi, S. Ali ShariatiFoxa1,"Abcam, ab55178",1:2000 Goat anti Sox17,"R&D Systems, AF1924",1:2000 Goat anti Oct3/4,"R&D Systems, AF1759",1:1000 Mouse anti Oct3/4,"Santa Cruz Biotechnology, sc-5279",1:200

(19) Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fateMolecular CellFebruary 1, 2024Satoshi Matsui, Marissa Granitto, Morgan Buckley, Katie Ludwig, Sandra Koigi, Joseph Shiley, William J. Zacharias, Christopher N. Mayhew, Hee-Woong Lim, Makiko IwafuchiRRID:AB_390153 PRDM1,Cell Signaling Technology,Cat#9115S; RRID:AB_2169699 OCT4/POU5F1,R&D systems,Cat#AF1759; RRID:AB_354975 PRDM14,Abcam,Cat#ab187881; RRID:N/A SALL4,Cell Signaling Technology,Cat#8459S; RRID:AB_10949321 RING1B/RNF2,Bethyl,Cat#A302-869A;

(20) Generation of five induced pluripotent stem cell lines from patients with MECP2 Duplication SyndromeStem cell researchFebruary 1, 2024Mendonca D, Cappuccio G, Sheppard J, Delacruz M, Bengtsson J, Carvalho CMB, Bajic A, Park H, Kim JJ, Jafar-Nejad P, et al.#MAB4381,RRID:AB_177638 ,Rabbit Anti-SOX2,1:50,Millipore #AB5603,RRID: AB_2286686 ,Goat Anti- OCT3/4,1:20,R&D Systems #AF1759,RRID:AB_354975 Endoderm marker,Goat Anti-human SOX17,10ug/ml,R&D Systems #963121,RRID:AB_355060 Ectoderm

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Oct-3/4 Antibody

Oct-3/4 Antibody

Oct-3/4 Antibody

Oct-3/4 Antibody

Oct-3/4 Antibody

Oct-3/4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Oct-3/4 Antibody from R&D Systems, a Bio-Techne Brand

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