Cripto Antibody AF1538 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCripto Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF1538
ApplicationsWestern Blot,Blockade of Receptor-ligand Interaction,Immunocytochemistry
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
HostSheep
ReactivityMouse
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetCripto
IsotypeIgG
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Cripto, Arg26-Gln150, Accession # P51865
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namecripto, EGF-CFC family member
NCBI Gene AliasesCR, CR-1, CRGF, TDGF1

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Cripto in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Cripto-1 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Biomanufacturing Recombinantly Expressed Cripto-1 Protein in Anchorage-Dependent Mammalian Cells Growing in Suspension Bioreactors within a Three-Dimensional Hydrogel MicrocarrierGels (Basel, Switzerland)March 18, 2023Lev R, Bar-Am O, Lati Y, Guardiola O, Minchiotti G, Seliktar D.concentration) or recombinant mouse activin receptor IB/Fc (for active protein concentration) (R&D systems AF1538 and 1477-AR, respectively). Briefly, 96–well plates were coated with 0.5 ng/mL of either activin RIB/ALK-4

(2) CRIPTO Is a Marker of Chemotherapy-Induced Stem Cell Expansion in Non-Small Cell Lung CancerFrontiers in oncologyJanuary 1, 2022Francescangeli F, De Angelis ML, Rossi R, Sette G, Eramo A, Boe A, Guardiola O, Tang T, Yu SC, Minchiotti G, et al.Assay ELISA–based assay was performed as previously described (42). Sheep anti-mouse CRIPTO Ab (R&D System, #AF1538) and mouse CRIPTO biotinylated Ab (R&D System, #BAF 1538) were used for coating and detection, respectively.

(3) Conditional Cripto overexpression in satellite cells promotes myogenic commitment and enhances early regenerationFrontiers in cell and developmental biologyJanuary 1, 2015Prezioso C, Iaconis S, Andolfi G, Zentilin L, Iavarone F, Guardiola O, Minchiotti G.Briefly ninety-six well plates were coated with 0.5 μg/ml of sheep anti-mouse Cripto Ab (R&D System, AF1538) in PBS overnight (O.N.) at 4°C. Unbinding sites were blocked with PBS-BSA 1% for 2 h at RT. After washing,

(4) Defects in GPI biosynthesis perturb Cripto signaling during forebrain development in two new mouse models of holoprosencephalyBiology openSeptember 15, 2012McKean DM, Niswander L.Western blots were performed with Smad2, phosphor-Smad2 (Cell Signaling 3122 and 3101) and Cripto (R&D Systems AF1538) antibodies. Blots were performed in triplicate and quantitated on Bio-Rad gel documentation system. Real time polymerase chain reaction

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Cripto Antibody

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Cripto Antibody

Cripto Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Cripto Antibody from R&D Systems, a Bio-Techne Brand

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