CD4 Antibody AF-379-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF-379-NA
NCBI Gene AliasesCD4mut, Leu-3, IMD79, T4, OKT4D
ApplicationsFlow Cyomtetry,IF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Neutralization,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Primate,Primate - Chlorocebus pygerythrus (Vervet Monkey),Xenograft
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetCD4
HostGoat
NCBI Full Gene NameCD4 molecule
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4, Lys26-Trp390, Accession # P01730
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human CD4 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this CD4 antibody be used for immunofluorescence microscopy in human samples?

Yes, the CD4 antibody from R&D Systems (AF-379-NA) has been utilized in immunofluorescence microscopy, as demonstrated in studies where it was used to detect CD4+ T cells in various human tissues, including lymph nodes and peripheral blood mononuclear cells.

Is this antibody effective for flow cytometry applications?

The CD4 antibody has been shown to work effectively in flow cytometry applications, as indicated by its use in studies that involved the analysis of CD4+ T cell populations in different experimental setups.

What species can this CD4 antibody detect?

This antibody is reactive with human (Homo sapiens), mouse (Mus musculus), and non-human primate species, making it versatile for various research applications involving these organisms.

Has this antibody been cited in any relevant scientific literature?

Yes, the CD4 antibody (AF-379-NA) has been cited in at least 28 scientific articles, indicating its widespread use and acceptance in the research community for studying CD4+ T cells and related immune responses.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pathology-oriented multiplexing enables integrative disease mappingNatureAugust 1, 2025Kuehl M, Okabayashi Y, Wong MN, Gernhold L, Gut G, Kaiser N, Schwerk M, Gräfe SK, Ma FY, Tanevski J, et al.

(2) Lymphedema pathogenesis involves antigen-driven expansion of CD4+ T cells in skinFront ImmunolAugust 1, 2025Adana-Christine Campbell, Annica R. Stull-Lane, Jung Eun Baik, Ananta Sarker, Jinyeon Shin, Gopika Ashokan, Hyeung Ju Park, Bracha L. Pollack, Pradhi Pakkerakari, Yollanda Franco Parisotto, et al.sectioned into 5-µm sections. The following anti-human antibodies were used for staining: CD4+ (1:1000; cat#AF379NA; R&D Systems), CD45RO (1:400; cat#MA511532; Thermo Fisher Scientific), and IR (1:1000; cat#AB137747;

(3) Stem-like T cells are associated with the pathogenesis of ulcerative colitis in humansNature immunologyJuly 1, 2024Li Y, Ramírez-Suástegui C, Harris R, Castañeda-Castro FE, Ascui G, Pérez-Jeldres T, Diaz A, Morong C, Giles DA, Chai J, et al.

(4) Transcriptome profiling and characterization of peritoneal metastasis ovarian cancer xenografts in humanized miceScientific ReportsMay 24, 2024Sung Wan Kang, Ji-young Lee, Ok-Ju Kang, Yong-Man Kim, Eun Kyung Choi, Shin-Wha Leeovernight at 4 °C with primary antibodies against anti-CD3 (EP449E, Abcam, Cambridge, MA, USA), anti-CD4 (AF-379-NA, R&D Systems, Minneapolis, MN, USA), anti-CD8 (4B11, Novus Biologicals, Centennial, CO, USA), and anti-PD-L1

(5) Smith-specific regulatory T cells halt the progression of lupus nephritisNature communicationsFebruary 6, 2024Eggenhuizen PJ, Cheong RMY, Lo C, Chang J, Ng BH, Ting YT, Monk JA, Loh KL, Broury A, Tay ESV, et al.

(6) Immuno-surveillance and protection of the human cochleaFrontiers in neurologyJanuary 1, 2024Liu W, Li H, Kämpfe Nordström C, Danckwardt-Lillieström N, Agrawal S, Ladak HM, Rask-Andersen H.States" CD11b,Monoclonal,1:50,Rabbit,AB52478,"Abcam, Cambridge, United Kingdom" CD4,Polyclonal,1:150,Goat,AF-379-NA,"R&DSystems, Minneapolis, United States" CD8α,Monoclonal,1:100,Mouse,MAB1509,"R&DSystems, Minneapolis,

(7) IL-33-primed human mast cells drive IL-9 production by CD4(+) effector T cells in an OX40L-dependent mannerFrontiers in immunologyJanuary 1, 2024Battut L, Leveque E, Valitutti S, Cenac N, Dietrich G, Espinosa E.Pharmingen), rabbit anti-OX40L (polyclonal rabbit, PA5-116057, Invitrogen), and anti-CD4 (polyclonal goat, AF-379-NA, R&D Systems). They were next incubated with matched AlexaFluor-conjugated secondary antibodies (Invitrogen)

(8) AZD5582 plus SIV-specific antibodies reduce lymph node viral reservoirs in antiretroviral therapy-suppressed macaquesNature medicineOctober 1, 2023Dashti A, Sukkestad S, Horner AM, Neja M, Siddiqi Z, Waller C, Goldy J, Monroe D, Lin A, Schoof N, et al.HNBP2-34039, Novus), mouse monoclonal anti-CD20 (1:500, M0755, DAKO) and goat polyclonal anti-CD4 (1:1,000, AF-379-NA, R&D Systems). Slides were washed and then incubated with secondary donkey anti-goat IgG-Alexa Fluor

(9) Tertiary lymphoid structures correlate with enhancement of antitumor immunity in esophageal squamous cell carcinomaBritish journal of cancerOctober 1, 2023Nakamura S, Ohuchida K, Hayashi M, Katayama N, Tsutsumi C, Yamada Y, Hisano K, Okuda S, Ohtsubo Y, Iwamoto C, et al.

(10) An agonistic anti-signal regulatory protein α antibody for chronic inflammatory diseasesCell reports. MedicineAugust 15, 2023Xie MM, Dai B, Hackney JA, Sun T, Zhang J, Jackman JK, Jeet S, Irizarry-Caro RA, Fu Y, Liang Y, et al.

(11) Circulating extracellular vesicles containing S100A9 reflect histopathology, immunophenotype and therapeutic responses of liver metastasis in colorectal cancer patientsBJC RepAugust 2, 2023Tsamchoe M, Lazaris A, Kim D, Krzywon L, Bloom J, Mayer T, Petrillo SK, Dejgaard K, Gao ZH, Rak J, et al.antibody anti-S100A9 (TFS, Catalogue PA5-82145, dilution 1:8000), anti-CD4 (R and D systems, Catalogue AF-379-NA, dilution 1:1200), anti-CD8 (Cell signalling, Catalogue 70306, dilution 1:200), anti-CD68 (Catalogue

(12) Polyclonal lymphoid expansion drives paraneoplastic autoimmunity in neuroblastomaCell ReportsAugust 2, 2023Miriam I. Rosenberg, Erez Greenstein, Martin Buchkovich, Ayelet Peres, Eric Santoni-Rugiu, Lei Yang, Martin Mikl, Zalman Vaksman, David L. Gibbs, Dan Reshef, et al.incubated with the slides for 1 h at room temperature, and b) Goat anti-human CD4 (1:400, R&D Systems AF-379-NA) and Rabbit anti-human CD8 (1:400, Thermo RB-9009-P0), which were both incubated for 1 h at room temperature.

(13) Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) modelExperimental & molecular medicineAugust 1, 2023Lee AR, Lee SY, Choi JW, Um IG, Na HS, Lee JH, Cho ML.Paraffin-embedded sections were incubated with 10% normal goat serum for 30 min and stained with anti-CD4 (AF-379-NA; R&D Systems), anti-IL-17 (MAB3171-100; R&D Systems), anti-IL-4 (PA5-25165; Thermo Fisher Scientific),

(14) Human galectin-9 promotes the expansion of HIV reservoirs in vivo in humanized miceAIDSMarch 15, 2023Zhe Yuan, Leila B. Giron, Colin Hart, Akwasi Gyampoh, Jane Koshy, Kai Ying Hong, Toshiro Niki, Thomas A. Premeaux, Lishomwa C. Ndhlovu, Claire Deleage, et al.containing 0.05% Tween-20, then incubated with primary antibody to CD4 (Goat anti-CD4, R&D system ref: AF-379-NA), CD68 (Rabbit anti-CD68, Sigma ref: HPA048982) and CD163 (rabbit anti-CD163, Lifespan Biosciences ref:

(15) Step-dose IL-7 treatment promotes systemic expansion of T cells and alters immune cell landscape in blood and lymph nodesiScienceFebruary 17, 2023Pandit H, Valentin A, Angel M, Deleage C, Bergamaschi C, Bear J, Sowder R, Felber BK, Pavlakis GN.for Tfh Ki67+ quantification IFA was performed by combining ON goat anti-CD4 (R&D system, 1:1000 cat#AF-379-NA), rabbit anti-KI67 (Thermo science, 1:200 cat# MA5-14520) and mouse anti-BCL6 (DAKO, 1:100) in TBS-tween.

(16) Human Galectin-9 Promotes the Expansion of HIV Reservoirs in vivo in Humanized MiceAIDSNovember 25, 2022Zhe YUAN, Leila B. GIRON, Colin HART, Akwasi GYAMPOH, Jane KOSHY, Kai Ying HONG, Toshiro NIKI, Thomas A. PREMEAUX, Lishomwa C. NDHLOVU, Claire DELEAGE, et al.containing 0.05% Tween-20, then incubated with primary antibody to CD4 (Goat anti-CD4, R&D system ref: AF-379-NA), CD68 (Rabbit anti-CD68, Sigma ref: HPA048982) and CD163 (rabbit anti-CD163, Lifespan Biosciences ref:

(17) Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal AdenocarcinomaJournal of personalized medicineApril 13, 2022Haddad R, Zlotnik O, Goshen-Lago T, Levi M, Brook E, Brenner B, Kundel Y, Ben-Aharon I, Kashtan H.

(18) E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune ResponsesVaccinesFebruary 15, 2022Sangare K, Helmold Hait S, Moore M, Hogge C, Hoang T, Rahman MA, Venzon DJ, LaBranche C, Montefiori D, Robert-Guroff M, et al.(Cat# 5420, Advanced Bioscience Laboratories, Inc. (ABL), Rockville, MD, USA); goat anti-hCD4 IgG (Cat# AF-379-NA, R&D Systems); mouse anti-DNA binding protein (DBP) (gift from David Ornelles, Wake Forest University,

(19) Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signalingiScienceJanuary 21, 2022Akusjärvi SS, Ambikan AT, Krishnan S, Gupta S, Sperk M, Végvári Á, Mikaeloff F, Healy K, Vesterbacka J, Nowak P, et al.min at RT using 4% PFA. Cells were blocked using 3% BSA and antibody detection of CD4 (R&D systems, #AF-379-NA) and Alexa 647 conjugated CD8 (Abcam, #ab196193) performed prior permeabilization 10 min using 0.2%

(20) Microbial Protein Binding to gC1qR Drives PLA2G1B-Induced CD4 T-Cell AnergyFrontiers in immunologyJanuary 1, 2022Pothlichet J, Meola A, Bugault F, Jeammet L, Savitt AG, Ghebrehiwet B, Touqui L, Pouletty P, Fiore F, Sauvanet A, et al.stained using anti-human CD4 (mouse anti-CD4 clone RPA-T4, 555344, BD Biosciences; or goat anti-CD4, AF-379-NA, R&D/Novus), followed by donkey anti-mouse-AlexaFluor488 (A21202, Thermofisher). Phosphorylation of

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CD4 Antibody

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CD4 Antibody

CD4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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CD4 Antibody from R&D Systems, a Bio-Techne Brand

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