Mouse EGFR Antibody AF1280 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse EGFR Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF1280
NCBI Gene Aliases9030024J15Rik, Errb1, wa-2, Erbb, Wa5, Errp, wa2
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Simple Western,Immunofluorescence,Immunocytochemistry
IsotypeIgG
TargetEGFR
Quantity100 ug (also 25 ug)
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Transgenic Mouse
Conjugate/TagUnconjugated
NCBI Full Gene Nameepidermal growth factor receptor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse EGFR, Leu25-Ser647, Accession # Q9EP98
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used to study the role of EGFR in hair follicle biology?

Yes, the EGFR antibody has been shown to be relevant in studies investigating the protective role of EGFR in hair follicle stem cell niches. Research indicates that disruption of EGFR signaling can lead to hypersensitivity in the JAK-STAT1 pathway, which compromises the stem cell niche and contributes to conditions such as scarring alopecia ().

What species can I use this antibody on for my experiments?

The EGFR antibody is reactive with mouse and human samples. It has been specifically demonstrated to detect mouse EGFR in various applications, including Western blotting and immunocytochemistry.

Is there any evidence of cross-reactivity with human EGFR when using this antibody?

The antibody exhibits approximately 20% cross-reactivity with recombinant human EGFR in direct ELISAs, which may be relevant for studies involving both mouse and human samples.

How has this antibody been utilized in scientific research?

The EGFR antibody has been cited in multiple scientific articles, including studies that explore the mechanisms of inflammation and hair follicle destruction associated with EGFR deficiency. It has been used in various applications such as Western blotting and immunohistochemistry to elucidate the role of EGFR in cellular signaling pathways and disease models.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Disruption of Epidermal Growth Factor Receptor Signaling in RPE Cells Increases Resistance to Ocular ToxoplasmosisInvestigative ophthalmology & visual scienceJune 2, 2025Pfaff A, Hubal A, Vos S, Bonilha V, Subauste CS.

(2) JAK-STAT1 as therapeutic target for EGFR deficiency-associated inflammation and scarring alopeciaEMBO molecular medicineDecember 1, 2024Strobl K, Klufa J, Jin R, Artner-Gent L, Krauß D, Novoszel P, Strobl J, Stary G, Vujic I, Griss J, et al.8, 1:200",Abcam,"Cat# ab25901, RRID: AB_448890" "Rabbit anti-mouse EGFR, 1:1000",Bio-Techne,"Cat# AF1280, RRID: AB_354717" "Rat anti-mouse E-Cadherin, 1:200",Abcam,"Cat# ab11512, RRID: AB_298118" "Rat anti-mouse

(3) Single nuclei RNA-seq of mouse placental labyrinth developmenteLifeNovember 3, 2020Marsh B, Blelloch R.Polyclonal antibody,R and D Systems,AF276,IF (1:100) Antibody,Anti-Egfr Polyclonal antibody,R and D Systems,AF1280,IF (1:100) Antibody,Anti-Gata1 Monoclonal antibody,Cell Signaling,3535,IF (1:100) Antibody,Anti-Hbegf

(4) Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal FatesCell reportsMay 5, 2020Micali N, Kim SK, Diaz-Bustamante M, Stein-O'Brien G, Seo S, Shin JH, Rash BG, Ma S, Wang Y, Olivares NA, et al.(AF1979), PDGFR alpha (AF1062; AF307), SOX2 (AF2018; MAB2018), SOX21 (AF3538), TuJ1 (MAB1195), EGFR (AF1280), O4 (MAB 1326) R&D Systems; GFAP (Z 0334) DAKO; HES5 (sc-13859), CUX1 (sc-13024), TLE4 (sc-9125), FGFR3

(5) Nanobody Targeting of Epidermal Growth Factor Receptor (EGFR) Ectodomain Variants Overcomes Resistance to Therapeutic EGFR AntibodiesMolecular cancer therapeuticsApril 1, 2019Tintelnot J, Baum N, Schultheiß C, Braig F, Trentmann M, Finter J, Fumey W, Bannas P, Fehse B, Riecken K, et al.

(6) Epidermal growth factor receptor promotes cerebral and retinal invasion by Toxoplasma gondiiScientific reportsJanuary 24, 2019Corcino YL, Portillo JC, Subauste CS.

(7) iRhom2 promotes lupus nephritis through TNF-α and EGFR signalingThe Journal of clinical investigationApril 2, 2018Qing X, Chinenov Y, Redecha P, Madaio M, Roelofs JJ, Farber G, Issuree PD, Donlin L, Mcllwain DR, Mak TW, et al.phosphorylated EGFR (p-EGFR) (Cell Signaling Technology, Y1068, catalog 3777), EGFR (R&D Systems, catalog AF1280), p-ERK1/2 (Cell Signaling Technology, T202/Y204, catalog 4370), ERK1/2 (Cell Signaling Technology,

(8) Endocannabinoid Signaling in Embryonic Neuronal Motility and Cell-Cell Contact - Role of mGluR5 and TRPC3 ChannelsNeuroscienceApril 1, 2018Turunen PM, Louhivuori LM, Louhivuori V, Kukkonen JP, Åkerman KE.

(9) Characterization of 7A7, an anti-mouse EGFR monoclonal antibody proposed to be the mouse equivalent of cetuximabOncotargetFebruary 23, 2018He X, Cruz JL, Joseph S, Pett N, Chew HY, Tuong ZK, Okano S, Kelly G, Veitch M, Simpson F, et al.(mouse, Absolute Antibody, Oxford, UK), 7A7 Fc Silent (mouse, Absolute Antibody), anti-mouse EGFR “AF1280” (polyclonal goat antibody, R&D Systems, New South Wales, Australia) or β-tubulin (clone 2-28-33, Invitrogen)

(10) The Immunogenicity and Anti-Tumor Efficacy of a Rationally Designed EGFR VaccineCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Cheng C, Deng L, Li R.

(11) EGF transactivation of Trk receptors regulates the migration of newborn cortical neuronsNature neuroscienceApril 1, 2013Puehringer D, Orel N, Lüningschrör P, Subramanian N, Herrmann T, Chao MV, Sendtner M.1:1,000), GFP (Santa Cruz, #sc-8334, 1:2,000), EGFR (Santa Cruz, #sc-03, #sc-81449, 1:1,000; R&D Systems, #AF1280, 1:200), MAP2 (Millipore, AB5622, 1:1,000), Doublecortin (DcX, Millipore, #AB2253, 1:6,000), Nestin

(12) T cells enhance stem-like properties and conditional malignancy in gliomasPloS oneJune 7, 2010Irvin DK, Jouanneau E, Duvall G, Zhang XX, Zhai Y, Sarayba D, Seksenyan A, Panwar A, Black KL, Wheeler CJ.

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