Mouse Netrin-1 Antibody AF1109 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Netrin-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF1109
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetNetrin-1
HostGoat
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Rat
IsotypeIgG
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Netrin-1, Val22-Ala603, Accession # AAC52971
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namenetrin 1
NCBI Gene AliasesNetrin-1

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunohistochemistry in mouse models?

Yes, the Netrin-1 antibody has been shown to work in immunohistochemistry applications. For instance, in a study, the antibody was used at a dilution of 1:100 for staining in mouse tissues, demonstrating its effectiveness in this application ().

Is there any evidence that this antibody can be used in studies related to cardiac development?

Yes, the Netrin-1 antibody has been utilized in research investigating cardiac development. A study indicated that Netrin-1 plays a crucial role in cardiac patterning and that its dosage impacts congenital heart defects, with the antibody being used for immunohistochemical analysis in this context ().

How does this antibody perform in studies related to cancer?

The Netrin-1 antibody has been employed in cancer research, particularly in studies examining its role in lymphomas. It was used to demonstrate that Netrin-1 acts as a survival factor for tumor cells in diffuse large B-cell lymphoma and mantle cell lymphoma, indicating its relevance in cancer biology ().

Can this antibody be used to study macrophage behavior in obesity-related research?

Yes, the Netrin-1 antibody has been applied in studies focusing on macrophage behavior in obesity. Research has shown that Netrin-1 is involved in the retention of macrophages in adipose tissue during obesity, and the antibody was used for immunofluorescence analysis to assess its expression in this context ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defectsNature Cardiovascular ResearchDecember 29, 2025Irfan S. Kathiriya, Martin H. Dominguez, Kavitha S. Rao, Jonathon M. Muncie-Vasic, W. Patrick Devine, Kevin M. Hu, Swetansu K. Hota, Bayardo I. Garay, Diego Quintero, Piyush Goyal, et al.performed using 5% donkey serum in 0.1% PBST at RT for 1–2 h. Anti-Netrin-1 antibody 1:500 (R&D Systems AF1109) in 1% BSA + 0.1% PBST was incubated at 4 °C overnight. After washing three times for 5 min per wash

(2) AQP5 Regulates Corneal Nerve Distribution Through NETRIN-1 and SLIT3Investigative Ophthalmology & Visual ScienceNovember 4, 2025Wenshuo Han, Yaning Liu, Peirong Song, Yu Gu, Fei Li, Wenbo Yan, Guohu Di, Dianqiang Wang, Peng Chen#9296-SL; R&D Systems), corneal epithelial extracts, or neutralizing antibodies against NETRIN-1 (4 µg/mL; #AF1109; R&D Systems) and SLIT3 (4 µg/mL; #AF3629; R&D Systems) were added for 48 hours to assess their effects

(3) Netrin-1 and B-cell maturation antigen expression in a large cohort of 361 lymphomas: sensitive and specific staining in plasmablastic lymphomas, and therapeutic perspectivesJ Pathol Clin ResApril 15, 2025Marie Donzel, Alexis Trecourt, Hervé Ghesquières, Thi-Thuy-Trinh Nguyen, Sara Dandash, Morgane Denis, Emeline Cros-Perrial, Juliette Fontaine, Charles Dumontet, Alexandra Traverse-Glehenprotocol and the present one may explain this discrepancy. Broutier et al employed a distinct antibody (AF1109, R&D Systems, Minneapolis, MN, USA), corresponding to a polyclonal goat IgG, which is validated for Western blot

(4) Netrin1 patterns the dorsal spinal cord through modulation of Bmp signalingCell ReportsNovember 26, 2024Sandy Alvarez, Sandeep Gupta, Yesica Mercado-Ayon, Kaitlyn Honeychurch, Cristian Rodriguez, Riki Kawaguchi, Samantha J. Butlertag,Abcam,Cat#ab32; RRID: AB_303599 Mouse anti GFP,Invitrogen,Cat#A-11120 Goat anti mouse netrin1,R&D Systems,Cat#AF1109; RRID: AB_2298775 Goat anti chicken netrin1,R&D Systems,Cat#AF128; RRID:AB_354716 Goat anti chicken

(5) Netrin-1 co-cross-linked hydrogel accelerates diabetic wound healing in situ by modulating macrophage heterogeneity and promoting angiogenesisBioactive materialsSeptember 1, 2024Shu F, Huang H, Xiao S, Xia Z, Zheng Y.experimental group were injected via the tail vein with mouse netrin-1-neutralizing antibody (10 mg/kg, AF1109, R&D Systems) on days 3 and 6, with the control mice injected with the isotype antibody (10 mg/kg, AB108C,

(6) A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defectsbioRxiv : the preprint server for biologyMarch 4, 2024Kathiriya IS, Dominguez MH, Rao KS, Muncie-Vasic JM, Devine WP, Hu KM, Hota SK, Garay BI, Quintero D, Goyal P, et al.performed using 5% donkey serum in 0.1% PBST at RT for 1–2hr. Anti-Netrin-1 antibody 1:500 (R&D Systems AF1109) in 1% BSA + 0.1% PBST was incubated at 4C overnight. After washing 3 x 5 min in 0.1% PBST, slides incubated

(7) Netrin1 patterns the dorsal spinal cord through modulation of Bmp signalingbioRxiv : the preprint server for biologyNovember 4, 2023Alvarez S, Gupta S, Honeychurch K, Mercado-Ayon Y, Kawaguchi R, Butler SJ.

(8) Netrin-1 Promotes the Immunosuppressive Activity of MDSCs in Colorectal CancerCancer immunology researchMay 3, 2023Xia X, Mao Z, Wang W, Ma J, Tian J, Wang S, Yin K.

(9) Restoring bone marrow niche function rejuvenates aged hematopoietic stem cells by reactivating the DNA Damage ResponseNature communicationsApril 10, 2023Ramalingam P, Gutkin MC, Poulos MG, Tillery T, Doughty C, Winiarski A, Freire AG, Rafii S, Redmond D, Butler JM.hours at room temperature. Sections were stained with an antibody raised against NTN1 (R&D Systems, AF1109, 1:100 dilution), and antibodies raised against mature hematopoietic cells (αGR1/CD11B/B220/CD3/CD41/TER119)

(10) Allosteric potentiation of GABA(A) receptor single-channel conductance by netrin-1 during neuronal-excitation-induced inhibitory synaptic homeostasisCell reportsNovember 1, 2022Chan ES, Ge Y, So YW, Bai YF, Liu L, Wang YT.

(11) Endothelial Unc5B controls blood-brain barrier integrityNature communicationsMarch 4, 2022Boyé K, Geraldo LH, Furtado J, Pibouin-Fragner L, Poulet M, Kim D, Nelson B, Xu Y, Jacob L, Maissa N, et al.(Invitrogen, 20221-1-AP, dilution: 1/300), Flrt2 (Novus bio, NBP2-43653, dilution: 1/500), Netrin-1 (R&D, AF1109, dilution: 1/500), Claudin-5 (Invitrogen, 35-2500, dilution: 1/1000), PLVAP (BD biosciences, 550563,

(12) The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in miceScientific reportsAugust 3, 2021Xiao M, Hu Z, Jiang H, Li C, Guo H, Fang W, Long X.

(13) DCC regulates astroglial development essential for telencephalic morphogenesis and corpus callosum formationeLifeApril 19, 2021Morcom L, Gobius I, Marsh AP, Suárez R, Lim JW, Bridges C, Ye Y, Fenlon LR, Zagar Y, Douglass AM, et al.Santa Cruz Biotechnology) and mouse NTN1 using a goat polyclonal antibody (1:500 U251 or 1:1000 N2A, AF1109, R&D Systems). GADPH was used as a loading control and detected using rabbit monoclonal anti-GADPH antibodies

(14) Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cellsNeuropharmacologyJune 1, 2017Yamahara K, Nakagawa T, Ito J, Kinoshita K, Omori K, Yamamoto N.

(15) Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal CordNeuronMay 17, 2017Varadarajan SG, Kong JH, Phan KD, Kao TJ, Panaitof SC, Cardin J, Eltzschig H, Kania A, Novitch BG, Butler SJ.1:200 (R&D Systems AF3076); mouse Dcc, 1:500 (R&D Systems AF844); mouse netrin1, 1:500 (R&D Systems AF1109); β-galactosidase, 1:2000 (Biogenesis 4600-1409); Sox2, 1:2000 (Santa Cruz Biotechnology #17320); Mouse:

(16) Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein ResponseCellular and molecular gastroenterology and hepatologyMay 1, 2016Lahlali T, Plissonnier ML, Romero-López C, Michelet M, Ducarouge B, Berzal-Herranz A, Zoulim F, Mehlen P, Parent R.β-actin (Sigma-Aldrich), total DAPK1 (Sigma-Aldrich), PR65β (Abcam, Cambridge, UK), netrin-1 antibody (AF1109; R&D Systems), eIF2α (Cell Signaling), Phospho-eIF2α (9721, Ser-51 specific; Cell Signaling), and α-tubulin

(17) Targeting netrin-1/DCC interaction in diffuse large B-cell and mantle cell lymphomasEMBO molecular medicineFebruary 1, 2016Broutier L, Creveaux M, Vial J, Tortereau A, Delcros JG, Chazot G, McCarron MJ, Léon S, Pangault C, Gadot N, et al.were then incubated in the autostainer (Dako) for 60 min at room temperature with netrin‐1 antibody (AF1109, R&D Systems), diluted at 1/100 in antibody diluent solution (Chemmate, Dako). After 3 PBS 1X rinses,

(18) Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesityNature medicineApril 1, 2014Ramkhelawon B, Hennessy EJ, Ménager M, Ray TD, Sheedy FJ, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, et al.overnight, embedded in paraffin and sectioned. The immunofluorescence analysis of netrin-1 (1:200, AF1109; R&D systems), caveolin-1 (1:500, 610059; BD bioscience), Unc5b (1:200, ab54430; Abcam), F4/80 (1:500,

(19) Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditionsArteriosclerosis, thrombosis, and vascular biologyMay 1, 2013van Gils JM, Ramkhelawon B, Fernandes L, Stewart MC, Guo L, Seibert T, Menezes GB, Cara DC, Chow C, Kinane TB, et al.

(20) Netrin-1 promotes glioblastoma cell invasiveness and angiogenesis by multiple pathways including activation of RhoA, cathepsin B, and cAMP-response element-binding proteinThe Journal of biological chemistryJanuary 25, 2013Shimizu A, Nakayama H, Wang P, König C, Akino T, Sandlund J, Coma S, Italiano JE Jr, Mammoto A, Bielenberg DR, et al.

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