Fig 2: LIF and LIFR protein in murine inter- and intra-implantation sites.A. LIF protein in cellular extracts quantified by ELISA (inter- and intra-implantation sites from n = 3 independent animals). B. LIFR protein in cellular extracts quantified by Western blot (inter- and intra-implantation sites from n = 3 independent animals).

Fig 3: LIF and LIFR immunolocalization in human endometrial biopsies.A. LIF immunolocalized to glandular epithelium (*), endothelial cells (a) and decidualized stromal cells (arrows) during the mid-secretory phase. B. LIF immunolocalized to decidual cells (arrows), endothelial cells (a) and glandular epithelium (*) in 1st trimester decidua. C. LIFR immunolocalized to glandular epithelium (*) and decidualized stromal cells (arrows) during the mid-secretory phase. D. LIFR immunolocalized to decidual cells (arrows) in 1st trimester decidua. Representative photomicrographs from n = 3. Scale, 100 µm; Insert, negative (IgG) control; a, spiral arterioles.

Fig 4: LIF (50 ng/ml) enhanced IL6 and IL15 secretion by in vitro decidualized HSEC.A. Cytokine secretion by first trimester decidual biopsies (n = 3). B. Cytokine secretion by HESC decidualized with estradiol (E) and medroxy-progesterone acetate (MPA) (n = 5). C. LIF treatment (50 ng/ml) significantly (*; compared to 0.5 ng/ml; p<0.05) induced IL6 secretion by decidualized HESC (n = 5). D. LIF treatment (50 ng/ml) had no significant effect (compared to 0.5 ng/ml; p = 0.222) on IL8 secretion by decidualized HESC (n = 5). E. LIF treatment (50 ng/ml) significantly (*; compared to 0.5 ng/ml; p<0.05) induced IL15 secretion by decidualized HESC (n = 5). F. LIF treatment (50 ng/ml) had no significant effect (compared to 0.5 ng/ml; p = 0.222) on MCP1 secretion by decidualized HESC (n = 5).

Fig 5: Leukemia inhibitory factor (LIF) activated STAT3 in HESC.Cells were treated with LIF for 15 min and with a LIF antagonist (LA) for 30 min prior to the addition of LIF. A. Representative immunoblot for pSTAT3 and total STAT3 in cell lysates from non-decidualized HESC (n = 3). B. Representative immunoblot for pSTAT3 and total STAT3 in cell lysates from decidualized HESC (n = 3).