CCL2/JE/MCP-1 Antibody AB-479-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCCL2/JE/MCP-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAB-479-NA
NCBI Gene AliasesMCP1, Scya2, MCAF, Sigje, HSMCR30, JE, HC11, SMC-CF, GDCF-2, SCYA2, MCP-1
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,In vivo assay,Neutralization,Cell Culture
Quantity1 mg
Conjugate/TagUnconjugated
IsotypeIgG
ReactivityHuman,Mouse,Transgenic Mouse
TargetCCL2/JE/MCP-1
HostGoat
NCBI Full Gene NameC-C motif chemokine ligand 2, chemokine (C-C motif) ligand 2
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose
ImmunogenE. coli-derived recombinant mouse CCL2/JE/MCP-1, Gln24-Arg96, Accession # Q5SVU3
ConcentrationLYOPH
PurityProtein A or G purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human MCP-1, recombinant mouse (rm MCP-5, and recombinant rat JE is observed. In Western blots, no cross-reactivity with rmEotaxin, rmMCP-5, or rmCCL7 is observed.

Sizes Available: 1 mg

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Adoptive cellular therapy prevents reconstitution of myeloid-derived suppressor cells in the glioma tumor microenvironmentNeurooncol AdvMarch 7, 2026John W Figg, Caitland Love, Sofia Stansbury, Dan Jin, Connor Francis, Bayli DiVita Dean, Alexandra Reid, Mia Engelbart, Illeana West, Laura Falceto Font, et al.the chemotaxis effect, chemokine was also plated with neutralizing antibody (αCCL2, R&D Systems, cat. AB-479-NA; αCCL12, R&D Systems, cat. MAB428-SP). Cells were plated at 150 µL/well in the upper Boyden chamber

(2) Cancer Manipulates Adjacent Adipose Tissue to Exploit Fatty Acids via HIF‐1α/CCL2/PPARα Axis: A Metabolic Circuit to Support Tumor ProgressionAdvanced ScienceJanuary 1, 2026Jeong‐Eun Yun, Jieun Seo, Jiwon Koh, Seock‐Ah Im, Ki Yong Hong, Yeseon Son, Do‐Won Jeong, Junji Fukuda, Jong‐Wan Park, Yang‐Sook Chuntreated to human cell lines at a concentration of 1 µg mL−1 and mouse neutralizing antibody‐CCL2 (#AB‐479‐NA, R&D systems) was treated to mouse cell lines at a concentration of 20 µg mL−1. Both neutralizing antibodies

(3) Immunological and Pathological Peculiarity of Severe Acute Respiratory Syndrome Coronavirus 2 Beta VariantMicrobiology spectrumOctober 26, 2022Lee S, Yoon GY, Lee SJ, Kwon YC, Moon HW, Kim YJ, Kim H, Lee W, Jeong GU, Kim C, et al.variant-infected mice were injected intraperitoneally with 5 mg/kg of body weight of CCL2/JE/MCP-1 antibody (AB-479-NA) and normal goat IgG control (AB-108-C) (R&D Systems) at 1, 3, and 6 dpi. Efforts were made to minimize

(4) ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activationCellular and molecular life sciences : CMLSFebruary 26, 2022Kim HJ, Kim WJ, Shin HR, Yoon HI, Moon JI, Lee E, Lim JM, Cho YD, Lee MH, Kim HG, et al.Santa Cruz Biotechnology), p21 (#2947; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse CCL2 antibody (AB-479-NA; R&D Systems, Inc, Minneapolis, MN, USA), mouse CCL5 antibody (AF478; R&D Systems), mouse CCL7 antibody

(5) B7-H3 Suppresses Antitumor Immunity via the CCL2-CCR2-M2 Macrophage Axis and Contributes to Ovarian Cancer ProgressionCancer immunology researchJanuary 1, 2022Miyamoto T, Murakami R, Hamanishi J, Tanigaki K, Hosoe Y, Mise N, Takamatsu S, Mise Y, Ukita M, Taki M, et al.

(6) Glioma-derived CCL2 and CCL7 mediate migration of immune suppressive CCR2(+)/CX3CR1(+) M-MDSCs into the tumor microenvironment in a redundant mannerFrontiers in immunologyJanuary 1, 2022Takacs GP, Kreiger CJ, Luo D, Tian G, Garcia JS, Deleyrolle LP, Mitchell DA, Harrison JK.(anti-CCL2, anti-CCL7, and normal goat IgG control) were purchased from R & D Systems (product number AB-479-NA, AF-456-NA, and AB-108-C) and stored following manufacture instructions. All wells that received neutralizing

(7) Myeloid cell-mediated targeting of LIF to dystrophic muscle causes transient increases in muscle fiber lesions by disrupting the recruitment and dispersion of macrophages in muscleHuman molecular geneticsDecember 27, 2021Flores I, Welc SS, Wehling-Henricks M, Tidball JG.with rat anti-F4/80 (1:50; Affimatrix eBioscience #14–8011) and goat anti-CCL2 (1:75; R&D Systems #AB-479-NA) overnight at 4°C and then incubated with a biotinylated anti-goat secondary antibody (1:200; Vector

(8) Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularizationCell death & diseaseNovember 27, 2020Liu X, Guo A, Tu Y, Li W, Li L, Liu W, Ju Y, Zhou Y, Sang A, Zhu M.and 5%CO2) for 24 h (hypoxia group), HMPL-013 (0.05 μmol/l for 24 h), CCL2-neutralizing antibody (#AB-479-NA, R&D Systems, USA; 5 μg/ml for the last 30 min), recombinant human CCL2 protein (#279-MC, R&D Systems;

(9) Mast Cells Localize in Hypoxic Zones of Tumors and Secrete CCL-2 under Hypoxia through Activation of L-Type Calcium ChannelsJournal of immunology (Baltimore, Md. : 1950)February 15, 2020Ramírez-Moreno IG, Ibarra-Sánchez A, Castillo-Arellano JI, Blank U, González-Espinosa C.tryptase (catalog no. AB-2378) were from Abcam (Cambridge, U.K.), and Abs against CCL-2 (catalog no. AB479-NA) were from R&D Systems (Minneapolis, MN). Abs against HIF1a (catalog no. MAB5382) were from Merck (Darmstadt,

(10) Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophyNature communicationsJune 26, 2019Welc SS, Flores I, Wehling-Henricks M, Ramos J, Wang Y, Bertoni C, Tidball JG.buffer for 1 h. Sections were then incubated with rat anti-F4/80 and goat anti-CCL2 (1:50, R&D Systems, AB-479-NA) or rabbit anti-TGFβ1 (1:100, Abcam, #ab92486) overnight at 4 °C. Sections were washed with PBS and

(11) Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARCOncogeneMay 1, 2019John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N.

(12) Chemokines CCL2, 3, 14 stimulate macrophage bone marrow homing, proliferation, and polarization in multiple myelomaOncotargetSeptember 15, 2015Li Y, Zheng Y, Li T, Wang Q, Qian J, Lu Y, Zhang M, Bi E, Yang M, Reu F, et al.antibodies to human CCL3 (MAB270), CCL14 (MAB3241) and CCL2 (MAB679), and mouse CCL3 (AB-450-NA), CCL2 (AB-479-NA) were purchased from R&D Systems. Immunohistochemistry antibodies to human CCL3, CCL14 and CCL2 and

(13) Retinol-binding protein 4 inhibits insulin signaling in adipocytes by inducing proinflammatory cytokines in macrophages through a c-Jun N-terminal kinase- and toll-like receptor 4-dependent and retinol-independent mechanismMolecular and cellular biologyMay 1, 2012Norseen J, Hosooka T, Hammarstedt A, Yore MM, Kant S, Aryal P, Kiernan UA, Phillips DA, Maruyama H, Kraus BJ, et al.chemoattractant protein 1 (MCP-1) were obtained from R&D Systems (catalog no. AB-410-NA, AB406-NA, and AB-479-NA). Cocultured 3T3L1 adipocytes and RAW264.7 macrophages were serum starved for 2 h, pretreated with 1

(14) IL-24 transgenic mice: in vivo evidence of overlapping functions for IL-20, IL-22, and IL-24 in the epidermisJournal of immunology (Baltimore, Md. : 1950)February 15, 2010He M, Liang P.CD3 (catalog SC-1127; Santa Cruz Biotechnology, Santa Cruz, CA) and goat anti-mouse MCP-1 (catalog AB-479-NA; R&D Systems, Minneapolis, MN) were both used at 1:500 dilutions. The rabbit envision +/DAB+ system

(15) Exacerbation of Established Pulmonary Fibrosis in a Murine Model by GammaherpesvirusAmerican Journal of Respiratory and Critical Care MedicineApril 1, 2008Tracy R. McMillan, Bethany B. Moore, Jason B. Weinberg, Kevin M. Vannella, W. Brad Fields, Paul J. Christensen, Linda F. van Dyk, Galen B. ToewsUniversity School of Medicine, St. Louis, MO) and CCL2 was detected using a goat anti-murine CCL2 IgG (no. AB-479-na; R&D Systems, Minneapolis, MN). Primary antibodies were detected using appropriate secondary reagents

(16) High pressure promotes monocyte adhesion to the vascular wallCirculation researchApril 27, 2007Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, et al.

(17) Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6Proceedings of the National Academy of Sciences of the United States of AmericaFebruary 13, 2007Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, et al.

(18) Low-Level Monocyte Chemoattractant Protein-1 Stimulation of Monocytes Leads to Tumor Formation in Nontumorigenic Melanoma CellsThe Journal of ImmunologyJune 1, 2001Mark Nesbit, Helmut Schaider, Thomas H. Miller, Meenhard Herlyn(Pharmacia). Western analysis indicated reactivity of a 13-kDa protein with mouse pAb specific for MCP-1 (AB479-NA; R&D Systems, Minneapolis, MN). BALB/c mice were immunized s.c. with 50 ␮g of recombinant protein in

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CCL2/JE/MCP-1 Antibody

CCL2/JE/MCP-1 Antibody

CCL2/JE/MCP-1 Antibody

CCL2/JE/MCP-1 Antibody

CCL2/JE/MCP-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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CCL2/JE/MCP-1 Antibody from R&D Systems, a Bio-Techne Brand

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