DLL4 Antibody AF1389 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemDLL4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF1389
ApplicationsIHC-F,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
Conjugate/TagUnconjugated
ReactivityChicken,Human,Mouse,Rat,Transgenic Mouse
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetDLL4
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
ClonalityPolyclonal
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
NCBI Full Gene Namedelta like canonical Notch ligand 4
NCBI Gene AliasesAOS6, delta4, hdelta2
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse DLL4, Ser28-Pro525, Accession # BAB18580

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DLL4 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunofluorescence applications in my studies on endothelial cells?

Yes, the DLL4 antibody has been successfully used in immunofluorescence applications, as demonstrated in studies such as those by Fernández-Chacón et al. (2019) and Ren et al. (2023), where it was utilized to visualize DLL4 expression in various cellular contexts.

What species does this antibody react with, and is there any cross-reactivity?

The DLL4 antibody is reactive with mouse, human, and rat species. It has been shown to exhibit approximately 50% cross-reactivity with recombinant human DLL4 in direct ELISAs, which may be relevant for studies involving human samples.

How has this antibody been utilized in research related to angiogenesis?

The DLL4 antibody has been employed in various studies investigating angiogenesis, including research by Izumi et al. (2012) and Kikuchi et al. (2011), where it was used to assess the role of DLL4 in endothelial cell behavior and vascular development, highlighting its importance in Notch signaling pathways.

Is there any specific storage requirement for this antibody after reconstitution?

Yes, after reconstitution, the DLL4 antibody should be stored under sterile conditions at -20 to -70 °C for up to six months. It can also be kept at 2 to 8 °C for one month under sterile conditions, but repeated freeze-thaw cycles should be avoided to maintain its integrity.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Retinoic acid regulates fetoplacental vascularization via notch signaling and a SEMA3E/F-PLEXIND1 axisiScienceApril 17, 2026Aleksandra Cwiek, Umadevi Paila, Zaneta Markowska, Nafiisha Genet, Madeline Jackson, Gael Genet, Shelby R. Cain, Jordon W. Aragon, Elizabeth A. Nelson, Ricardo Moraes Borges, et al.1:100) (Cat# 550274; BD, Franklin Lakes, NJ), arterial ECs (DLL4- Mouse DLL4 Antibody conc. 1:200) (Cat# AF1389; R & D Systems, Minneapolis, MN), and venous ECs (Endomucin-Endomucin Rat anti Mouse Monoclonal Antibody

(2) Bmpr2 Loss in the Vascular Endothelium Enhances Neoangiogenesis and Growth of Lung Metastatic Lesions in a Mouse Model of Breast CancerJournal of Cellular BiochemistryNovember 12, 2025Jordan A. Harry, Sofia I. Skebo, Devon V. Cole, Vincent Dinh, M. Martin VandenBroek, Abby K. Lee, Natasha Camacho, Hollie N. Piepjohn, Victoria Hoskin, Kimberly J. Laverty, et al.segments. Lung sections were also stained for DLL4 using a 1:400 dilution of goat‐α‐DLL4 antibody (AF1389; Bio‐Techne, Minneapolis, MN) and a 1:500 dilution of donkey‐α‐goat AF647‐conjugated secondary (Invitrogen,

(3) Mechanical forces pattern endocardial Notch activation via mTORC2-PKC pathwayeLifeFebruary 11, 2025Mu Y, Hu S, Liu X, Tang X, Lin J, Shi H.,R&D Systems,"Cat# AF1002, RRID:AB_2077789",IF(1:400) antibody,Dll4 (Goat polyclona),R&D Systems,"Cat# AF1389, RRID:AB_354770",IF(1:200) antibody,Troponin T (RV-C2) (Rabbit monoclonal),DSHB,"Cat# RV-C2, RRID:AB_2240831",IF(1:200) antibody,Sox9

(4) Specialized post-arterial capillaries facilitate adult bone remodellingNature cell biologyDecember 1, 2024Mohanakrishnan V, Sivaraj KK, Jeong HW, Bovay E, Dharmalingam B, Bixel MG, Dinh VV, Petkova M, Paredes Ugarte I, Kuo YT, et al.

(5) Analyzing embryo dormancy at single-cell resolution reveals dynamic transcriptional responses and activation of integrin-Yap/Taz prosurvival signalingCell Stem CellSeptember 5, 2024Rui Chen, Rui Fan, Fei Chen, Niraimathi Govindasamy, Heike Brinkmann, Martin Stehling, Ralf H. Adams, Hyun-Woo Jeong, Ivan Bedzhovanti-Cdcp1,R&D systems,"Cat# AF4515-SP, RRID: AB_2078800" Mouse polyclonal anti-Dll4,R&D systems,"Cat# AF1389, RRID: AB_354770" Hamster monoclonal anti-CD29-FITC,BD Biosciences,"Cat# 561796, RRID: AB_10894590" Rat

(6) Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signalingNeuronJune 19, 2024Biswas S, Shahriar S, Bachay G, Arvanitis P, Jamoul D, Brunken WJ, Agalliu D.anti-cleaved Caspase-3 (Cell Signaling Technology, Cat #9664S, 1:500), Goat anti-DLL4 (R & D Systems, Cat #AF1389, 1:200), rabbit anti-Claudin-5 (ThermoFisher Scientific, Cat #34-1600, 1:500), rabbit anti-SOX9 (Millipore Sigma

(7) Role of endothelial PDGFB in arterio-venous malformations pathogenesisAngiogenesisMay 1, 2024Yanzhu Lin, Johannes Gahn, Kuheli Banerjee, Gergana Dobreva, Mahak Singhal, Alexandre Dubrac, Roxana Olaanti-Jag1 (#AF599, 1:200, R&D systems), anti-Endomucin (#sc-65495, 1:200, Santa Cruz), anti-Dll4 (#AF1389, 1:200, R&D systems), anti-phospho-SMAD3 (#ab52903, 1:100, Abcam), anti-phospho-SMAD1/5 (#13820S, 1:100,

(8) Dopamine signaling from ganglion cells directs layer-specific angiogenesis in the retinaCurrent biology : CBSeptember 25, 2023Liang JH, Akhanov V, Ho A, Tawfik M, D'Souza SP, Cameron MA, Lang RA, Samuel MA.Jagged1,Santa Cruz Biotechnology,"Cat# sc-390177, RRID:AB_2892141" Goat polyclonal Dll4,R&D Systems,"Cat# AF1389, RRID:AB_354770" Chicken polyclonal anti-GFP,Abcam,Cat#ab13970; RRID:AB_300798 Rabbit monoclonal VEGFR2,Cell Signaling Technology

(9) Hypoxia-induced AFAP1L1 regulates pathological neovascularization via the YAP-DLL4-NOTCH axisJournal of translational medicineSeptember 22, 2023Ren JS, Bai W, Ding JJ, Ge HM, Wang SY, Chen X, Jiang Q.anti-AFAP1L1 (Santa Cruz Biotechnology, sc-376700; IF 1:50, WB 1:1000), mouse DLL4 Antibody (R&D Systems, AF1389; IF 1:200), rabbit anti-CD31(Abcam, ab28364,1:200), mouse anti-DLL4 (Santa Cruz Biotechnology, sc-365429;

(10) Targeting FSCN1 with an oral small-molecule inhibitor for treating ocular neovascularizationJournal of translational medicineAugust 18, 2023Bai W, Ren JS, Xia M, Zhao Y, Ding JJ, Chen X, Jiang Q.anti-p-VEGFR2 (Tyr1175) (Cell Signaling Technology, 2478; WB 1:1000), goat anti-DLL4 (R&D Systems, AF1389; IF 1:200), and mouse anti-β‐actin (Millipore, A5441; WB 1:1000). The following secondary antibodies

(11) Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signalingbioRxiv : the preprint server for biologyJuly 11, 2023Biswas S, Shahriar S, Bachay G, Arvanitis P, Brunken WJ, Agalliu D.

(12) Active Remodeling of Capillary Endothelium via Cancer Cell-Derived MMP9 Promotes Metastatic Brain ColonizationCancer researchApril 14, 2023Karreman MA, Bauer AT, Solecki G, Berghoff AS, Mayer CD, Frey K, Hebach N, Feinauer MJ, Schieber NL, Tehranian C, et al.anti-Collagen IV (1:100, AB756P, Sigma-Aldrich, USA, RRID:AB_2276457), anti-Delta-like ligand 4 (Dll4, 1:100, AF1389, R&D Systems, USA, RRID:AB_354770), anti-Endocan/ESM-1 (1:100, AF1999, R&D Systems, USA, RRID:AB_2101810),

(13) PI(4,5)P(2)-dependent regulation of endothelial tip cell specification contributes to angiogenesisScience advancesMarch 31, 2023Davies EM, Gurung R, Le KQ, Roan KTT, Harvey RP, Mitchell GM, Schwarz Q, Mitchell CA.PI(4,5)P2 (Z-PO45), and PI(4)P (Z-P004) antibodies were from Echelon Biosciences. Goat anti-DLL4 antibody (AF1389) was from R&D Systems. Collagen IV (21501470) was from Bio-Rad. Pan-actin (MS-1295-P) was from Neomarkers.

(14) Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infectionCell reportsSeptember 27, 2022Hou Q, Jia J, Lin J, Zhu L, Xie S, Yu Q, Li Y.

(15) Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem CellsStem cells translational medicineJuly 20, 2022Lee CK, Park BY, Jo T, Park CH, Kim JH, Chung KJ, Kim YH, Park DY, Kim IK.MD, USA) for 1 h and then incubated overnight with the following primary antibodies: anti-Dll4 (goat; AF1389; R&D Systems, Minneapolis, USA), anti-CD31 (hamster; 2H8; Millipore, Billerica, USA), anti-perilipin

(16) Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodentsDevelopment (Cambridge, England)July 1, 2022Katreddi RR, Taroc EZM, Hicks SM, Lin JM, Liu S, Xiang M, Forni PE.Signaling Technology), goat anti-Dll4 (*with antigen retrieval 1:50 and without antigen retrieval 1:25, AF1389, R&D Systems), *rabbit anti-NICD activated (1:75, D3B8, Cell Signaling Technology), *mouse anti-DsRd

(17) Gatekeeping role of Nf2/Merlin in vascular tip EC induction through suppression of VEGFR2 internalizationScience advancesJune 10, 2022Bae JH, Yang MJ, Jeong SH, Kim J, Hong SP, Kim JW, Kim YH, Koh GY.ab92513), goat anti-ESM1 polyclonal (R&D Systems, AF1999), goat anti-Dll4 polyclonal (R&D Systems, AF1389), rabbit anti-pERK1/2 monoclonal [Cell Signaling Technology (CST), #4370], rabbit anti-TAZ polyclonal

(18) A novel peptide inhibitor of Dll4-Notch1 signalling and its pro-angiogenic functionsBritish journal of pharmacologyApril 1, 2022Zhu G, Lin Y, Ge T, Singh S, Liu H, Fan L, Wang S, Rhen J, Jiang D, Lyu Y, et al.(1:100; Abcam, ab15200), mouse anti-aSMA-Cy3 (1:500; Sigma, C6198), goat anti-Dll4 (1:100; R&D Systems, AF1389) and goat anti-Jag1 (1:100; Sigma, J4127). After wash in PBS with three times, appropriate Alexa Fluor

(19) Induction of osteogenesis by bone-targeted Notch activationeLifeFebruary 4, 2022Xu C, Dinh VV, Kruse K, Jeong HW, Watson EC, Adams S, Berkenfeld F, Stehling M, Rasouli SJ, Fan R, et al.R&D Systems, 1:100), rabbit anti-Osteocalcin (LS-C17044, LifeSpan BioSciences, 1:100), goat anti-Dll4 (AF1389, R&D Systems, 1:100), goat anti-Jag1 (J4127, Sigma, 1:100), goat anti-PDGFRβ (AF1042, R&D, 1:100), rabbit

(20) Vascular Sema3E-Plexin-D1 Signaling Reactivation Promotes Post-stroke Recovery through VEGF Downregulation in MiceTranslational stroke researchFebruary 1, 2022Yu R, Kim NS, Li Y, Jeong JY, Park SJ, Zhou B, Oh WJ.Receptor 2 (55B11) antibody (1:1000, 2479, Cell Signaling), goat polyclonal anti-Dll4 antibody (1:1000, AF1389, R&D systems), rabbit monoclonal anti-b-actin antibody (1:5000, 5125, Cell Signaling), rabbit polyclonal

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DLL4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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DLL4 Antibody from R&D Systems, a Bio-Techne Brand

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