ErbB2/Her2 Antibody AF1129 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemErbB2/Her2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1129
NCBI Gene Aliasesc-ERB-2, NGL, MLN-19, CD340, VSCN2, TKR1, HER-2, p185(erbB2), HER2, NEU, HER-2/neu, MLN 19, c-ERB2
ApplicationsELISA Development,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Inhibition of Cell Growth,Immunocytochemistry,CyTOF-ready,ELISA Capture
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Transgenic Mouse
Conjugate/TagUnconjugated
HostGoat
TargetErbB2/Her2
Quantity100 ug (also 25 ug)
IsotypeIgG
NCBI Full Gene Nameerb-b2 receptor tyrosine kinase 2
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human ErbB2/Her2, Thr23-Thr652, Accession # P04626
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human ErbB2/Her2 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse ErbB2 is observed and less than 5% cross-reactivity with recombinant human (rh) ErbB3, rhErbB4, and rhEGF R is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for Western blotting applications?

Yes, the ErbB2/Her2 antibody has been successfully utilized in Western blotting, as evidenced by its application in studies where it was used to detect ErbB2 expression in various cell lines, including those overexpressing the receptor (, ).

What is the specificity of this antibody when detecting ErbB2?

The ErbB2/Her2 antibody specifically detects human ErbB2 with less than 20% cross-reactivity with recombinant mouse ErbB2 and minimal cross-reactivity (less than 5%) with other human ErbB receptors such as ErbB3 and ErbB4 ().

Has this antibody been cited in any studies related to cancer research?

Yes, the ErbB2/Her2 antibody has been cited in at least 18 scientific articles, including studies investigating its role in breast cancer and the mechanisms of ErbB2 signaling and degradation (, ).

Is this antibody suitable for use in immunofluorescence applications?

The ErbB2/Her2 antibody has been used in immunofluorescence studies, where it was employed to visualize ErbB2 expression in various cancer models, confirming its utility in this application ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Quantitative immunohistochemistry and the use of cellular calibrators for HER2 receptor number determinationScientific ReportsMarch 23, 2026Kevin McKinski, Bin Chenthe plate was washed again, and anti-HER2 goat pAb from R&D Systems (Minneapolis, MN, USA; Catalog AF1129) was diluted to 2 µg/mL and added to the plate for 1 h. The plate was washed a final time and then 2x

(2) Switchable Retargeting of Lentiviral Vectors Through a VSV-G-Binding Adapter MoleculeVirusesNovember 29, 2025Vladislav A. Zhuchkov, Marat P. Valikhov, Yulia E. Kravchenko, Elena I. Frolova, Stepan P. ChumakovCFL555-labeled anti-ERBB2 antibodies. 2.5. Antibodies and Flow Cytometry Goat anti-human ErbB2/Her2 antibody AF1129 (R&D Systems, Minneapolis, MN, USA) and mouse anti-goat IgG-CFL 555 (Santa Cruz Biotechnology, Dallas,

(3) HER1 (EGFR) and/or HER2 Inclusion Potentiates the Antitumor Effect Elicited by a HER3-Specific Monovalent VaccineMolecular cancer therapeuticsAugust 1, 2025Bermudez-Abreut E, Bergado-Báez G, Fundora-Barrios T, Arencibia-Perezleo J, Lopez Medinilla A, Chao L, Sanchez Ramirez B.

(4) Antitumor activity of PAbs generated by immunization with a novel HER3-targeting protein-based vaccine candidate in preclinical modelsFrontiers in OncologyOctober 16, 2024Ernesto Bermúdez-Abreut, Gretchen Bergado Báez, Melissa Martínez Pestano, Giuseppe Attanasio, Carlos Yordan Gonzales Castillo, Diana Rosa Hernández Fernández, Rydell Alvarez-Arzola, Andrea Alimonti, Belinda Sánchez-Ramírezmembranes were incubated overnight with primary antibodies: anti-HER3 (AF234, R&D Systems, USA), anti-HER2 (AF1129, R&D Systems), anti-HER1 (AF231, R&D Systems), or ß-actin (MAB8929, R&D Systems). This was followed

(5) Nanotechnology and machine learning enable circulating tumor cells as a reliable biomarker for radiotherapy responses of gastrointestinal cancer patientsBiosensors and BioelectronicsApril 15, 2023Michael J. Poellmann, Jiyoon Bu, Stanley Liu, Andrew Z. Wang, Steven N. Seyedin, Chandrikha Chandrasekharan, Heejoo Hong, YoungSoo Kim, Joseph M. Caster, Seungpyo Hong(HEPES) were obtained from MilliporeSigma (St. Louis, MO). The capture antibodies aEPCAM (AF960), aHER2 (AF1129), aEGFR (AF231), along with recombinant human E-Selectin/CD62E chimera protein, CF were obtained from

(6) Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer PatientsBiosensorsMarch 7, 2023Wignarajah S, Chianella I, Tothill IE.

(7) Cell surface protein aggregation triggers endocytosis to maintain plasma membrane proteostasisNature communicationsFebruary 28, 2023Paul D, Stern O, Vallis Y, Dhillon J, Buchanan A, McMahon H.(2367) and EGFR (4267) were from Cell Signaling Technology. An antibody recognising the HER2 ectodomain (AF1129) was from R&D Systems. Antibodies recognising VAV1 (ab245440), VAV2 (ab52640), VAV3 (ab52938), GFP (ab290),

(8) Multiomic Profiling Identified EGF Receptor Signaling as a Potential Inhibitor of Type I Interferon Response in Models of Oncolytic Therapy by Vesicular Stomatitis VirusInternational journal of molecular sciencesMay 8, 2022Nikitina AS, Lipatova AV, Goncharov AO, Kliuchnikova AA, Pyatnitskiy MA, Kuznetsova KG, Hamad A, Vorobyev PO, Alekseeva ON, Mahmoud M, et al.sc-47778 diluted at 1:3500 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and ErbB2/Her2 Antibody AF1129 diluted at 1:3000 (R&D Systems, Minneapolis, MN, USA) were used for actin control and HER2 detection,

(9) CHIP/STUB1 Ubiquitin Ligase Functions as a Negative Regulator of ErbB2 by Promoting Its Early Post-Biosynthesis DegradationCancersAugust 4, 2021Luan H, Bailey TA, Clubb RJ, Mohapatra BC, Bhat AM, Chakraborty S, Islam N, Mushtaq I, Storck MD, Raja SM, et al.through Covance Research Products, Denver, PA, USA. ErbB2 immuno-staining was carried out using the AF1129 antibody from R&D Systems, Minneapolis, MN, USA, or Alexa Fluor-488 or 647-conjugated mouse anti-human

(10) Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspiratesNPJ breast cancerJuly 2, 2021Joh DY, Heggestad JT, Zhang S, Anderson GR, Bhattacharyya J, Wardell SE, Wall SA, Cheng AB, Albarghouthi F, Liu J, et al.transfer radical polymerization as described previously36. HER2 capture/detection Ab pairs (cat# MAB1129/AF1129, respectively, R&D Systems, Inc.) were then deposited onto POEGMA-coated slides as follows. The cAbs

(11) The deubiquitylase USP2 maintains ErbB2 abundance via counteracting endocytic degradation and represents a therapeutic target in ErbB2-positive breast cancerCell death and differentiationSeptember 1, 2020Zhang J, Liu S, Li Q, Shi Y, Wu Y, Liu F, Wang S, Zaky MY, Yousuf W, Sun Q, et al.(clones A-2 and 9G6) antibodies were purchased from Santa Cruz Biotechnology (CA, USA). Goat anti-ErbB2 (AF1129) antibody was obtained from R&D systems. Rabbit anti-USP2 antibody was purchased from Abgent. Mouse

(12) Direct protein quantification in complex sample solutions by surface-engineered nanorod probesScientific reportsJuly 6, 2017Schrittwieser S, Pelaz B, Parak WJ, Lentijo-Mozo S, Soulantica K, Dieckhoff J, Ludwig F, Schotter J.drug (Ab-a) or commercial HER2 antibodies (Ab-b) purchased from R&D Systems under catalogue number AF1129. Here, Ab-a is a monoclonal antibody, while Ab-b is of polyclonal nature. In a next step, 100 bovine

(13) Stable and Potent Selenomab-Drug ConjugatesCell chemical biologyApril 20, 2017Li X, Nelson CG, Nair RR, Hazlehurst L, Moroni T, Martinez-Acedo P, Nanna AR, Hymel D, Burke TR Jr, Rader C.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Goat anti-human HER2 polyclonal antibodies,R&D Systems,Cat#AF1129; RRID: AB_354617 Goat IgG,Jackson ImmunoResearch Laboratories,Cat#005-000-003; RRID: AB_2336985 Alexa

(14) Marked enhancement of lysosomal targeting and efficacy of ErbB2-targeted drug delivery by HSP90 inhibitionOncotargetMarch 1, 2016Raja SM, Desale SS, Mohapatra B, Luan H, Soni K, Zhang J, Storck MA, Feng D, Bielecki TA, Band V, et al.for Western blotting studies from BD Pharmingen™ (San Diego, CA); goat anti-human-ErbB2 polyclonal (AF1129) used for flow cytometry and immunofluorescence studies from R&D Systems (Minneapolis, MN); mouse monoclonal

(15) Polypeptide-based nanogels co-encapsulating a synergistic combination of doxorubicin with 17-AAG show potent anti-tumor activity in ErbB2-driven breast cancer modelsJournal of controlled release : official journal of the Controlled Release SocietyJune 28, 2015Desale SS, Raja SM, Kim JO, Mohapatra B, Soni KS, Luan H, Williams SH, Bielecki TA, Feng D, Storck M, et al.CA); for flow cytometry and immunofluorescence studies, the goat anti-human-ErbB2 polyclonal antibody (AF1129) was from R&D Systems (Minneapolis, MN); the mouse monoclonal anti-phosphotyrosine (anti-pY; 4G10) was

(16) Engineering a single ubiquitin ligase for the selective degradation of all activated ErbB receptor tyrosine kinasesOncogeneFebruary 20, 2014Kong F, Zhang J, Li Y, Hao X, Ren X, Li H, Zhou P.

(17) Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligationNew biotechnologyJanuary 25, 2013Gu GJ, Friedman M, Jost C, Johnsson K, Kamali-Moghaddam M, Plückthun A, Landegren U, Söderberg O.non-conjugated SNAP-DARPin band. The anti-HER2 antibody pertuzumab and the anti-HER2 polyclonal antibody (AF1129, R&D Systems) were conjugated separately with aldehyde-modified priming and non-priming oligonucleotides

(18) Novel method for differentiation between Trastuzumab and host adaptive responseMolecular immunologySeptember 1, 2011Denisova G, Jenkins K, Evelegh C, Bramson J.

(19) ErbB2 Is Necessary for ErbB4 Ligands to Stimulate Oncogenic Activities in Models of Human Breast CancerGenes & cancerAugust 1, 2011Mill CP, Zordan MD, Rothenberg SM, Settleman J, Leary JF, Riese DJ 2nd.(Santa Cruz Biotechnology; used to assess endogenous ErbB2 expression in MCF7 and T47D cells), the AF1129 goat anti-ErbB2 polyclonal antibody (R&D Systems, Minneapolis, MN; used to assess ectopic ErbB2 expression

(20) Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected MimotopesPLoS ONEApril 23, 2009Galina Denisova, Dimitri Denisov, Carole Evelegh, Michaela Weissgram, Jochen Beck, Stephen Ronan Foley, Jonathan Lorne Bramson(BD Biosceinces, catalog# 610161) and a polyclonal serum against human HER-2 (R&D Systems, catalog #AF1129) Measurement of HER-2 specific antibodies by ELISA 96 well plates (Nunc, New York, USA) were coated

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