Ret Antibody AF1485 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRet Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1485
NCBI Gene AliasesCDHF12, HSCR1, PTC, RET-ELE1, CDHR16, MEN2B, MEN2A, MTC1
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetRet
Quantity100 ug (also 25 ug)
IsotypeIgG
ReactivityHuman,Mouse,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
HostGoat
NCBI Full Gene Nameret proto-oncogene
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human Ret, Leu29-Arg635, Accession # P07949
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Ret in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Ret is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunofluorescence in human induced pluripotent stem cell-derived neurons?

Yes, the Ret Antibody has been shown to work in immunofluorescence characterization of human induced pluripotent stem cell-derived sensory neurons without the need for permeabilization, as demonstrated in a study by Alich et al. (2023) where it was used at a dilution of 1:200.

What species does this antibody react with, and how specific is it?

The Ret Antibody is reactive with Homo sapiens (human), mouse, and non-human primates. It has approximately 10% cross-reactivity with recombinant mouse Ret in direct ELISAs, indicating a degree of specificity for human Ret.

Has this antibody been utilized in any studies related to Parkinson's disease?

Yes, the Ret Antibody was used in a study by Chu et al. (2020) to assess RET expression in post-mortem brain tissues of patients with advanced Parkinson's disease following gene therapy. The study highlighted the expression of RET in nigral neurons, which is relevant for understanding the pathology of the disease.

What is the recommended storage condition for this antibody after reconstitution?

After reconstitution, the Ret Antibody should be stored under sterile conditions at -20 to -70 °C for up to six months. It can also be kept at 2 to 8 °C for one month under sterile conditions.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Spatially patterned kidney assembloids recapitulate progenitor self-assembly and enable high-fidelity in vivo disease modelingCell Stem CellOctober 2, 2025Biao Huang, Pedro Medina, Jincan He, Zipeng Zeng, Sunghyun Kim, Janet Romo, Kari Koppitch, Chennan C. Zhang, Georgina Gyarmati, Yohan Park, et al.,Cell Signaling Technology,Cat# 3223; RRID: AB_2238465 Goat polyclonal anti-RET (human),R&D Systems,Cat# AF1485; RRID: AB_354820 Rabbit Monoclonal anti-SOX9,Abcam,Cat# ab185230; RRID: AB_2715497 Rat Monoclonal

(2) Human fetal kidney organoids model early human nephrogenesis and Notch-driven cell fateThe EMBO JournalJuly 21, 2025Michael Namestnikov, Osnat Cohen-Zontag, Dorit Omer, Yehudit Gnatek, Sanja Goldberg, Thomas Vincent, Swati Singh, Yair Shiber, Tal Rafaeli Yehudai, Hadas Volkov, et al.Vector Laboratories), anti-EPCAM (1:500, CBL251, Merck Millipore, 1:500, ab71916, Abcam), anti-RET (1:100, AF1485, R&D Systems), anti-KI-67 (1:100, MONX10283, Monosan), and anti-PODXL (1:100, ab150358, Abcam). After

(3) Integrating collecting systems in human kidney organoids through fusion of distal nephron to ureteric budCell stem cellJuly 3, 2025Shi M, Crouse B, Sundaram N, Pode Shakked N, Thorner K, King NM, Dutta P, Ester L, Zhang W, Govindarajah V, et al.RRID:AB_10012062 Mouse anti-PRKCZ,Santa Cruz,Cat# sc-17781; RRID:AB_628148 Goat anti-RET,R&D Systems,Cat# AF1485; RRID:AB_354820 Rabbit anti-SIX1,Cell Signaling Technology,Cat# 12891; RRID:AB_2753209 Rabbit anti-SIX2,Proteintech,Cat#

(4) Induction of Human Pruriceptors from Pluripotent Stem Cells via Transcription FactorsbioRxivJune 18, 2025Hisato Iriki, Ruiqi Hu, Xu Li, Erdene Baljinnyam, Carina Habich, Ichiro Imanishi, Loan Miller, Kavya Chegireddy, Laraib Iqbal Malik, Daniel Yassky, et al.anti-IL31RA (Abcam, AB113498, 1:200), anti-OSMR (LSBio, LS-B11477–50, 1:200), anti-RET (Novus biologicals, AF1485, 1:200)]. After incubation, cells were washed three times with PBS and incubated for 1 h at RT in the

(5) Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric budbioRxiv : the preprint server for biologySeptember 20, 2024Shi M, Crouse B, Sundaram N, Pode Shakked N, Ester L, Zhang W, Janakiram V, Kopan R, Helmrath MA, Bonventre JV, et al.

(6) Human Nephrogenesis can Persist Beyond 40 Postnatal Days in Preterm InfantsKidney international reportsFebruary 1, 2024Carpenter J, Yarlagadda S, VandenHeuvel KA, Ding L, Schuh MP.Rosemont, IL), rabbit anti-WT1 (Abcam, ab202639, Cambridge, United Kingdom), goat anti-RET (R&D Systems, AF1485, Minneapolis, MN), guinea pig antikeratin K8 (PROGEN Biotechnik, GP-K8), and mouse anti-E-cadherin (BD Biosciences

(7) Emergence of nociceptive functionality and opioid signaling in human induced pluripotent stem cell-derived sensory neuronsPainAugust 1, 2023Röderer P, Belu A, Heidrich L, Siobal M, Isensee J, Prolingheuer J, Janocha E, Valdor M, Hagendorf S, Bahrenberg G, et al.1:100, abcam, ab85015; TrkA: 1:50, Alomone labs, ANT-018; hRet: 1:100, R&D Systems, Minneapolis, MN, AF1485). Images for quantification purposes were taken with the InCell Analyzer 2200 plate microscope (GE Healthcare,

(8) Directed differentiation of ureteric bud and collecting duct organoids from human pluripotent stem cellsNature protocolsAugust 1, 2023Shi M, Fu P, Bonventre JV, McCracken KW.systems,610181,1:500,1:500,AB_397580 SOX9,Novusbio,NBP1-85551,1:200,1:200,AB_11002706 RET,R&D Systems,AF1485,1:200,1:200,AB_354820 AQP2,Millipore,178612,1:200,Not tested,AB_2056864 Donkey anti-goat AF488,Jackson Immuno

(9) Bringing to light the physiological and pathological firing patterns of human induced pluripotent stem cell-derived neurons using optical recordingsFrontiers in cellular neuroscienceJanuary 1, 2022Alich TC, Röderer P, Szalontai B, Golcuk K, Tariq S, Peitz M, Brüstle O, Mody I.solutions (mouse-anti-Nav1.7, Abcam, ab85015, 1:100; goat-anti-hRet, R&D Systems, Minneapolis, MN, USA, AF1485, 1:200). After washing, coverslips were mounted on high precision cover glasses using Fluoromount-G

(10) GDNF signaling in subjects with minimal motor deficits and Parkinson's diseaseNeurobiology of diseaseJune 1, 2021Chu Y, Kordower JH.

(11) Expansion of Human iPSC-Derived Ureteric Bud Organoids with Repeated Branching PotentialCell reportsJuly 28, 2020Mae SI, Ryosaka M, Sakamoto S, Matsuse K, Nozaki A, Igami M, Kabai R, Watanabe A, Osafune K.

(12) Gut Microbiota Modulate CD8 T Cell Responses to Influence Colitis-Associated TumorigenesisCell reportsApril 7, 2020Yu AI, Zhao L, Eaton KA, Ho S, Chen J, Poe S, Becker J, Gonzalez A, McKinstry D, Hasso M, et al.

(13) Long-term post-mortem studies following neurturin gene therapy in patients with advanced Parkinson's diseaseBrain : a journal of neurologyMarch 1, 2020Chu Y, Bartus RT, Manfredsson FP, Olanow CW, Kordower JH.supernatant was then used in lieu of the primary antibody. The adsorption experiment of RET antibody (AF1485; R&D system) with a recombinant RET protein (1168-CR; R&D system) was performed using the above method.

(14) Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidneyDevelopment (Cambridge, England)August 30, 2018Menon R, Otto EA, Kokoruda A, Zhou J, Zhang Z, Yoon E, Chen YC, Troyanskaya O, Spence JR, Kretzler M, et al.

(15) Functional RET G691S polymorphism in cutaneous malignant melanomaOncogeneAugust 27, 2009Narita N, Tanemura A, Murali R, Scolyer RA, Huang S, Arigami T, Yanagita S, Chong KK, Thompson JF, Morton DL, et al.

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Ret Antibody

Ret Antibody

Ret Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Reviews

Ret Antibody from R&D Systems, a Bio-Techne Brand

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