HGFR/c-MET Antibody AF527 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHGFR/c-MET Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF527
ApplicationsELISA Development,ELISA Development (Capture),Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Rat,Transgenic Mouse
HostGoat
TargetHGFR/c-MET
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse HGF R/c-MET, Glu25-Asn929, Accession # P16056
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameMET proto-oncogene, receptor tyrosine kinase
NCBI Gene AliasesAUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) HGF R and less than 1% cross-reactivity with rhMSP R is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pericytes are organ-specific regulators of tissue morphogenesisNat CommunMay 12, 2026Seyed Javad Rasouli, Kai Kruse, Rodrigo Diéguez-Hurtado, Parisa Ghanbari, Anusha Aravamudhan, Mara E. Pitulescu, Ralf H. Adamsab76013), rat anti-ICAM2/CD102 (1:100, BD Pharmingen, 553326), goat anti-HGFR/c-Met (1:100, R&D Systems, AF527), rabbit anti ERG (1:100, Abcam, ab110639), rabbit anti-Cleaved Caspase-3 (1:100, Cell Signaling, #9664),

(2) Acquired and intrinsic resistance to vemurafenib in BRAFV600E -driven melanoma brain metastasesFEBS Open BioJanuary 1, 2024Ping Zhang, Laura Esmee Kuil, Levi Conrad Maria Buil, Stephan Freriks, Jos Hendrik Beijnen, Olaf van Tellingen, Mark Cornelis de Gooijer3169; Cell Signaling Technology), phospho‐MET (1 : 150; 3077; Cell Signaling Technology), MET (1 : 100; AF527; R&D Systems, Minneapolis, MN, USA), phospho‐IGF1R (1 : 40 000; sc‐101703; Santa Cruz Biotechnology,

(3) SOX9-positive pituitary stem cells differ according to their position in the gland and maintenance of their progeny depends on contextScience advancesOctober 6, 2023Rizzoti K, Chakravarty P, Sheridan D, Lovell-Badge R.

(4) Novel rAAV vector mediated intrathecal HGF delivery has an impact on neuroimmune modulation in the ALS motor cortex with TDP-43 pathologyGene therapyAugust 1, 2023Genç B, Nho B, Seung H, Helmold B, Park H, Gözütok Ö, Kim S, Park J, Ye S, Lee H, et al.

(5) Fibroblast-derived Hgf controls recruitment and expansion of muscle during morphogenesis of the mammalian diaphragmeLifeSeptember 26, 2022Sefton EM, Gallardo M, Tobin CE, Collins BC, Colasanto MP, Merrell AJ, Kardon G.2837,Working concentration:0.48 µg/ml Antibody,Anti-HGFR/c-MET (goat polyclonal),R&D Systems,Cat# AF527,Working concentration:10 µg/ml Antibody,"Anti-PHOSPHO-MET, PE Conjugate (rabbit monoclonal)",Cell Signaling,Cat#

(6) The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal modelBMC cancerJune 17, 2022Jiang AN, Wang B, Wang S, Zhao K, Wu H, Yan K, Wu W, Yang W.Signaling, Danvers, MA), HIF-α (MAB1536-SP, R&D Systems), IL-6 (AF-406-SP, R&D Systems) and c-Met (AF527, R&D Systems). The positive cells of different markers were analyzed according to IHC/IF staining of

(7) Two-Chains Tissue Plasminogen Activator Unifies Met and NMDA Receptor Signalling to Control Neuronal SurvivalInternational journal of molecular sciencesDecember 15, 2021Hedou E, Douceau S, Chevilley A, Varangot A, Thiebaut AM, Triniac H, Bardou I, Ali C, Maillasson M, Crepaldi T, et al.anti-phospho-MET (Y1234/1235) (Santa Cruz Biotechnology, sc-101736, diluted 1:1000), anti-Met (R&D systems, AF527, diluted 1:1000), anti-HGF (Invitrogen, SBF5, diluted 1:1000), anti-GluN2B (abcam, ab65783, diluted

(8) Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosiseLifeMarch 15, 2021Weinstock A, Rahman K, Yaacov O, Nishi H, Menon P, Nikain CA, Garabedian ML, Pena S, Akbar N, Sansbury BE, et al.anti-TGFb (521707; Biolegend), 0.1 mg/mL anti-gp130 from (MAB4682; R&D Systems), 1 mg/mL anti-HGFR (AF527; R&D Systems), 10 mg/mL anti-IL10R (112707; Biolegend), 0.1 mM AMG487 CXCR3 inhibitor (4487; Tocris

(9) MET Receptor Tyrosine Kinase Regulates Lifespan Ultrasonic Vocalization and Vagal Motor Neuron DevelopmentFrontiers in neuroscienceJanuary 1, 2021Kamitakahara AK, Ali Marandi Ghoddousi R, Lanjewar AL, Magalong VM, Wu HH, Levitt P.ab13970, RRID:AB_300798), goat anti-MET receptor tyrosine kinase (MET) (1:500, R and D Systems Cat# AF527, RRID:AB_355414), mouse monoclonal anti-Neurofilament H 1G9 (NF) (1:500, Pennypacker et al., 1991),

(10) Aberrant activation of hepatocyte growth factor/MET signaling promotes β-catenin-mediated prostatic tumorigenesisThe Journal of biological chemistryJanuary 10, 2020Aldahl J, Mi J, Pineda A, Kim WK, Olson A, Hooker E, He Y, Yu EJ, Le V, Lee DH, et al.

(11) Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumor aggressiveness in the murine prostateThe Journal of biological chemistryDecember 28, 2018Mi J, Hooker E, Balog S, Zeng H, Johnson DT, He Y, Yu EJ, Wu H, Le V, Lee DH, et al.

(12) Pulmonary pericytes regulate lung morphogenesisNature communicationsJune 22, 2018Kato K, Diéguez-Hurtado R, Park DY, Hong SP, Kato-Azuma S, Adams S, Stehling M, Trappmann B, Wrana JL, Koh GY, et al.(553326, BD Biosciences, 1:100), rabbit anti-Tropoelastin (ab21600, abcam, 1:200), goat anti-HGF R/c-MET (AF527, R&D Systems, 1:200), rat anti-RAGE (MAB1179, R&D Systems, 1:200), rabbit anti-ERG (ab110639, abcam,

(13) Distinct projection targets define subpopulations of mouse brainstem vagal neurons that express the autism-associated MET receptor tyrosine kinaseThe Journal of comparative neurologyDecember 15, 2017Kamitakahara A, Wu HH, Levitt P.(GFP) (Abcam Cat# ab13970, RRID:AB_300798), MET receptor tyrosine kinase (MET) (R and D Systems Cat# AF527, RRID:AB_355414), Neurofilament (DSHB Cat# 2H3, RRID:AB_531793), or Tyrosine Hydroxylase (TH) (Millipore

(14) Specific Connectivity and Unique Molecular Identity of MET Receptor Tyrosine Kinase Expressing Serotonergic Neurons in the Caudal Dorsal Raphe NucleiACS chemical neuroscienceMay 17, 2017Kast RJ, Wu HH, Williams P, Gaspar P, Levitt P.Fluorescent Protein (anti-GFP) (Abcam Cat# ab13970), goat anti-HGF receptor (anti-MET) (R and D Systems Cat# AF527), and rabbit anti-serotonin (anti-5HT, Sigma). Secondary antibodies used were as follows: Alexa 647

(15) Hepatocyte Growth Factor-c-MET Signaling Mediates the Development of Nonsensory Structures of the Mammalian Cochlea and HearingThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 3, 2016Shibata S, Miwa T, Wu HH, Levitt P, Ohyama T.upon request. For immunohistochemistry, antibodies used in this study were c-Met (R&D Systems, catalog #AF527; 1:100), Claudin 11 (Abcam, catalog #ab53041; RRID, AB_2276205; 1:500), Kcnq1 (Sigma, catalog #SAB2501224;

(16) Receptor tyrosine kinase Met promotes cell survival via kinase-independent maintenance of integrin α3β1Molecular biology of the cellAugust 1, 2016Tesfay L, Schulz VV, Frank SB, Lamb LE, Miranti CK.Becton-Dickinson Transduction Labs (Franklin Lakes, NJ). Polyclonal goat mouse-specific anti-Met antibody (AF527) was from R&D Systems (Minneapolis, MN). Integrin α6 polyclonal antibody was provided by Anne Cress

(17) Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranesNature communicationsJune 23, 2016Barrow-McGee R, Kishi N, Joffre C, Ménard L, Hervieu A, Bakhouche BA, Noval AJ, Mai A, Guzmán C, Robbez-Masson L, et al.polyclonals anti-: human c-Met extracellular domain (AF276, R&D Systems), mouse c-Met extracellular domain (AF527, R&D Systems), early endosome antigen 1 (EEA1) (Santa Cruz Biotechnology). —Rat monoclonal anti-mouse

(18) cMET inhibitor crizotinib impairs angiogenesis and reduces tumor burden in the C3(1)-Tag model of basal-like breast cancerSpringerPlusJanuary 1, 2016Cozzo AJ, Sundaram S, Zattra O, Qin Y, Freemerman AJ, Essaid L, Darr DB, Montgomery SA, McNaughton KK, Ezzell JA, et al.anti-mouse phospho-cMET (Abcam #ab5662, Lot GR159296-1; 1:4000); Goat anti-mouse cMET (R&D Systems #AF527, Lot CTB0310091; 1:1000) diluted in Renoir Red Diluent (BM #PD904H). Additional reagents included biotin-conjugated

(19) Hepatocyte Growth Factor and MET Support Mouse Enteric Nervous System Development, the Peristaltic Response, and Intestinal Epithelial Proliferation in Response to InjuryThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 19, 2015Avetisyan M, Wang H, Schill EM, Bery S, Grider JR, Hassell JA, Stappenbeck T, Heuckeroth RO.1:800, Neuromics), RET (R787) (Rabbit, 1:100; #18121, Immuno-Biological Laboratories), MET (goat, 1:100; AF527, R&D Systems), CGRP (rabbit, 1:100; #C8198, Sigma-Aldrich), phosphohistone 3 (pH3; rabbit, 1:800; #AB06-570,

(20) MET is required for the recruitment of anti-tumoural neutrophilsNatureJune 18, 2015Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson AA, Wauters E, Walmsley S, Prenen H, Granot Z, et al.Briefly, 96-well microtiter plates (MaxiSorp, Nunc) were coated with 1 μg/ml of anti-mouse MET antibody (AF527, R&D Systems) overnight at 4°C and then incubated for 2 hours at room temperature (RT) in blocking buffer

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HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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HGFR/c-MET Antibody from R&D Systems, a Bio-Techne Brand

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