EphB2 Antibody AF467 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEphB2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF467
NCBI Gene AliasesDrt, Hek5, Qek5, BDPLT22, Prkm5, DRT, ETECK, Nuk, PCBC, EPHT3, Tyro5, EK5, Erk, Cek5, Sek3, CAPB, ERK
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Proximity Ligation Assay,In vivo assay,Simple Western,Neutralization,Immunofluorescence,Immunocytochemistry,CyTOF-ready,Functional Assay
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetEphB2
ReactivityHuman,Mouse,Rat,Transgenic Mouse,Frog - Xenopus (African Clawed Frog)
Conjugate/TagUnconjugated
IsotypeIgG
Quantity100 ug (also 25 ug)
NCBI Full Gene NameEPH receptor B2, Eph receptor B2
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse EphB2, Val27-Lys548, Accession # P54763
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunofluorescence applications?

Yes, the EphB2 antibody has been shown to work in immunofluorescence applications, as demonstrated in studies where it was utilized for staining cells to analyze EphB2 expression in various contexts, including endothelial cells and neuronal cultures.

What species does this antibody react with?

The EphB2 antibody is reactive with human, mouse, rat, and Xenopus/Amphibian species, making it versatile for studies involving these organisms.

Is there any evidence of this antibody being used in studies related to nerve regeneration?

Yes, the EphB2 antibody has been cited in research focusing on peripheral nerve regeneration, where it was used to investigate the role of EphB2 in Schwann cell signaling and axon guidance during nerve repair processes.

How does this antibody perform in Western blot applications?

The EphB2 antibody has been effectively used in Western blotting, as indicated by its application in studies that required the detection of EphB2 protein levels in various experimental setups, including those examining signaling pathways in nerve cells.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) An AI-integrated organoid platform enables high-throughput functional evaluation of bioactive metal ionsBioact MaterJune 13, 2026Yi Shao, Yongfeng Wang, Yan Wang, Jingru Xu, Ping Li, Jiaqi Zhao, Xuan Du, Xueqiang Liu, Shihui Xu, Lu Wang, et al.Abcam, Shanghai, China), CDX2 (MAB3665-SP, R&D Systems), KRT20 (ab64090, Abcam, Shanghai, China), EphB2 (AF467, R&D Systems), MUC2 (Abcam, ab272692), Ki-67 (ab15580, Abcam, Shanghai, China) and cleaved Caspase-3

(2) A combined strategy of EGFR-MET bispecific antibody and HER3 ADC to overcome osimertinib resistance in NSCLCCell Oncol (Dordr)January 27, 2026Yuanyuan Wang, Haoyue Guo, Ruoshuang Han, Yuhan Wu, Taiping He, Meng Diao, Anwen Xiong, Fei Zhou, Lei Cheng, Chao Zhao, et al.(Novus, Littleton, CO, USA, Cat#NB600-724PE, PE, RRID: AB_3195682), MET (Novus, Cat#NBP2-44306AF647, AF467, RRID: AB_3308587) and HER3 (BD Pharmingen, Cat#751787, BV650, RRID: AB_2875762). Cellular specimens

(3) Specific neuroblast-derived signals control both cell migration and fate in the rostral migratory streambioRxivJanuary 16, 2026Wei Zhou, James R. Munoz, Hsin-Yi Henry Henry Ho, Kenji Hanamura, Matthew B. Dalvaanti-Doublecortin,Cell Signaling Technology,Cat# 4604; RRID: AB_10570650 Goat anti-EphB1,R&D Systems,Cat# AF467; RRID: AB_355262 Goat anti-EphB2,R&D Systems,Cat# AF467; RRID: AB_355262 Goat anti-EphB3,R&D Systems,Cat#

(4) Diet-induced MASH liver fibrosis promoted by EphB2 can be targeted by small molecule tetramerization inhibitorsbioRxiv : the preprint server for biologyDecember 8, 2025Yoshigi M, Kamdem SD, Wang H, Khambete A, Sprouse F, Kameni EM, Atiogue E, Pellizzari S, Evason KJ, Helms MN, et al.

(5) Multi-receptor targeted therapy of breast cancer and brain metastases with a novel QUAD-drug conjugateBreast Cancer ResOctober 27, 2025Waldemar Debinski, Kaitlin N. Fink, John Rossmeisl, Ryan T. Mott, Kounosuke Watabe, Ralph D'Agostino, Alexandra Thomas, Denise Herpaihouse), EphA3 (MyBiosource, San Diego, CA, cat# MBS821916), EphB2 (R&D Systems, Minneapolis, MN, cat# AF467), IL-13RA2 (HUABIO Woburn Massachusetts cat# HA722051), PLK-1 antibody 1:1000 (Genetex, cat# GTX15779

(6) Ligand preference of EphB2 receptor is selectively regulated by N-glycosylationiScienceMay 16, 2025Lyu C, Yuan L, Yang Y, Zhang D, Hu W, Zhao K, Ding Y, Chen W, Xiao K, Chen Y, et al.EphB2 protein expression level, confirmed by Western blot using an anti-EphB2 antibody (R&D SYSTEMS, AF467). After a 72-hour infection, biotinylated ephrin-A5 or ephrin-B2 proteins were pre-clustered with Streptavidin-Alexa

(7) Intestinal crypt microbiota modulates intestinal stem cell turnover and tumorigenesis via indole acetic acidNature microbiologyMarch 1, 2025Zhang S, Peng L, Goswami S, Li Y, Dang H, Xing S, Feng P, Nigro G, Liu Y, Ma Y, et al.

(8) Tofacitinib and budesonide treatment affect stemness and chemokine release in IBD patient-derived colonoidsScientific reportsJanuary 30, 2025Sridhar A, Bakke I, Gopalakrishnan S, Osoble NMM, Hammarqvist EP, Pettersen HPS, Sandvik AK, Østvik AE, Hansen MD, Bruland T.LGR5 Mouse mAb (1:1000, #MA5-25644, Invitrogen), EPH Receptor B2 (EphB2) Goat mAb (1:1000 dilution, #AF467, R&D Systems), Phospho-MLKL (Ser358) (E7G7P) Rabbit mAb (1:1000 dilution, #18640, Cell Signaling Technology),

(9) Hepatic SerpinA1 improves energy and glucose metabolism through regulation of preadipocyte proliferation and UCP1 expressionNature communicationsNovember 12, 2024Okagawa S, Sakaguchi M, Okubo Y, Takekuma Y, Igata M, Kondo T, Takeda N, Araki K, Brandao BB, Qian WJ, et al.

(10) BMPRII(+) neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord developmentNeural regeneration researchFebruary 1, 2024Weible Ii MW, Lovelace MD, Mundell HD, Pang TWR, Chan-Ling T.raised against the human extracellular domain, used at 10 μg/mL; R&D Systems, Stillwater, MN, USA, Cat# AF467, RRID: AB_355622), anti-CD34 (vascular lineage marker; monoclonal antibody (1:50; mouse IgG1), Bio-Rad

(11) Identification of new ciliary signaling pathways in the brain and insights into neurological disordersbioRxiv : the preprint server for biologyDecember 21, 2023Loukil A, Ebright E, Uezu A, Gao Y, Soderling SH, Goetz SC.Bio-Techne, AF473); Anti-Ephrin-B2 (IF: 1:50, Bio-Techne, AF496); Anti-EphB2 (IF: 1:50, Bio-Techne, AF467); Anti-EphA4 (IF: 1:50, Proteintech, 21875-1-AP); Anti-CSNK1G1 (IF: 1:50, OriGene, TA806333S); Anti-ROBO2

(12) M2 macrophage-derived cathepsin S promotes peripheral nerve regeneration via fibroblast-Schwann cell-signaling relayJournal of neuroinflammationNovember 9, 2023Oshima E, Hayashi Y, Xie Z, Sato H, Hitomi S, Shibuta I, Urata K, Ni J, Iwata K, Shirota T, et al.Ephrin-B2 (rabbit polyclonal, 1:50, HPA008999, Sigma-Aldrich), or EphB2 (mouse polyclonal, 1:1000, AF467, R&D systems) in PBS overnight at 4 °C. For the staining of primary cultured macrophages, TG neurons,

(13) Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor functionbioRxiv : the preprint server for biologyOctober 28, 2023Chang C, Banerjee SL, Park SS, Zhang X, Cotnoir-White D, Opperman KJ, Desbois M, Grill B, Kania A.

(14) GluN3A subunit tunes NMDA receptor synaptic trafficking and content during postnatal brain developmentCell reportsMay 30, 2023González-González IM, Gray JA, Ferreira J, Conde-Dusman MJ, Bouchet D, Perez-Otaño I, Groc L.Upstate; anti-GluN2A, M264 Sigma; anti-GluN2B, AB1557, Sigma; anti-PSD95: CP35, Calbiochem; anti-EphB2R AF467 R&D System), membranes were incubated with secondary HRP-conjugated anti-mouse or anti-rabbit (LNA934V

(15) Role of EphB2/ephrin-B1 signalling in the development and progression of obesity-associated colorectal cancerOncology lettersSeptember 1, 2022Suzuki Y, Okabayashi K, Hasegawa H, Tsuruta M, Seishima R, Tokuda T, Kitagawa Y.

(16) EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networksCell reportsJuly 5, 2022Banerjee SL, Lessard F, Chartier FJM, Jacquet K, Osornio-Hernandez AI, Teyssier V, Ghani K, Lavoie N, Lavoie JN, Caruso M, et al.

(17) Retrograde movements determine effective stem cell numbers in the intestineNatureJuly 1, 2022Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, Bruens L, Webb AT, Laskaris D, Oost KC, Lafirenze SJA, Annusver K, Messal HA, et al.06-570). To visualise Ephrin B2 and B3, intestines were stained with Ephrin B2-antibody (1:100 R&D system, AF467) and Ephrin B3-antibody (1:100 R&D system, AF432). Stainings were finalized by incubation with Alexa

(18) Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injuryExperimental eye researchJune 1, 2022Guaiquil VH, Xiao C, Lara D, Dimailig G, Zhou Q.SC-25641,Goat anti rabbit Alexa green 448,1:400,ThermoFisher A11008 EphB2,Goat,1:200,all,R&D Systems AF467,Donkey anti goat TRITC,1:400,Jackson Immunoresearch 705-025-147 EphB4,Goat,1:200,all,R&D Systems AF446,Donkey

(19) Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sortingCell reportsMarch 15, 2022Lewis AE, Kuwahara A, Franzosi J, Bush JO.

(20) Tyrosine phosphorylation of DEPTOR functions as a molecular switch to activate mTOR signalingThe Journal of biological chemistryNovember 1, 2021M Gagné L, Morin N, Lavoie N, Bisson N, Lambert JP, Mallette FA, Huot MÉ.Santa Cruz; 1:1000 dilution), anti-EPHB2 D2X2I (83029; Cell Signaling; 1:1000 dilution), anti-EPHB2 (AF467; R&D Systems; 1:200 dilution), and anti-SYK D3ZE1 (13198; Cell Signaling; 1:500 dilution). IPs For

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EphB2 Antibody

EphB2 Antibody

EphB2 Antibody

EphB2 Antibody

EphB2 Antibody

EphB2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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EphB2 Antibody from R&D Systems, a Bio-Techne Brand

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