Fig 1: PLD2 promotes cell invasion through the regulation of ANG in SKRC52 and SKRC59 cells. A, Evaluation of the mRNA expression of ANG of SKRC52 and SKRC59 cells infected with scramble or PLD2 shRNA. P‐value was assessed by Student's t test (*P < .05, **P < .01, ***P < .001). B, The mRNA expression of ANG in 52 clear cell renal cell carcinoma (ccRCC) patients. P‐value was assessed by Wilcoxon rank sum test. C, Analysis of cell invasion ability after treatment with ANG‐neutralized antibody to control IgG. P‐value was assessed by Student's t test (*P < .05, **P < .01, ***P < .001). D, Analysis of cell invasion ability after treatment with recombinant human ANG protein to control (0.1%BSA). P‐value was assessed by Student's t test (*P < .05, **P < .01, ***P < .001). E, The effect of PA on the mRNA expression of ANG in SKRC52 and SKRC59 infected with PLD2 shRNA. P‐value was assessed by Student's t test (*P < .05, **P < .01, ***P < .001)