Islet-1 Antibody AF1837 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIslet-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1837
NCBI Gene AliasesISLET1, Isl-1
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry
Conjugate/TagUnconjugated
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityAvian - Chicken,Chicken,Human,Mouse,Transgenic Mouse
TargetIslet-1
IsotypeIgG
Quantity100 ug (also 25 ug)
NCBI Full Gene NameISL LIM homeobox 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Islet-1, Met4-Ala349, Accession # P61371
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study motor neuron development in human stem cells?

Yes, the Islet-1 antibody has been utilized in studies involving the differentiation of human induced pluripotent stem cells into motor neurons, demonstrating its effectiveness in marking these cells during development ().

What is the specificity of the Islet-1 antibody in immunostaining applications?

The Islet-1 antibody has been shown to specifically detect human Islet-1 in immunostaining applications, including immunohistochemistry, with a dilution of 1:1000 being effective for visualizing neuronal populations in the mouse brain ().

Is the Islet-1 antibody suitable for use in flow cytometry?

Yes, the Islet-1 antibody has been cited in studies that employed flow cytometry to analyze neuronal populations, indicating its suitability for this application ().

How does the Islet-1 antibody perform in terms of cross-reactivity with other proteins?

The Islet-1 antibody exhibits approximately 10% cross-reactivity with recombinant human Islet-2 in direct ELISAs, which is important to consider when interpreting results in experiments involving these closely related proteins.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Neurotrophin-3 produced by motor neurons non-cell autonomously regulate the development of pre-motor interneurons in the developing spinal cordbioRxivFebruary 20, 2026Andrea Angla-Navarro, Ana Dominguez Dominguez Bajo, Mathilde Toch, Cédric Francius, Maria Hidalgo-Figueroa, Jingwen Zhang, Olivier Schakman, Manon Martin, Xiuqian Mu, René Rezsohazy, et al.(Cell signaling #5852) at 1:200, guinea pig anti-MafA (Gierl et al., 2006) at 1:500, goat anti-Isl1 (R&D #AF1837) at 1:1000, rabbit anti-MafA (Novus Biological #NB400–137) at 1:500, mouse anti-MNR2 (DSHB #81.5C10)

(2) In vivo human embryonic spinal cord atlas validates stem cell–derived human dorsal interneurons and reveals ASD spinal signaturesbioRxivDecember 26, 2025Sandeep Gupta, Eric Heinrichs, Cristian Rodriguez, Emily Friedman, Salena Gallardo, Talin Dermirjie, Teny Panosian, Keith Phan, Anooshik Tahmasian, Yahir Verdin, et al.1:50), FOXD3 (guinea pig, a gift from Thomas Mueller, Germany, 1:10,000), ISL1 (goat; R&D systems, AF1837; 1:500), PAX2 (rabbit; Invitrogen, 71–6000; 1:500), LMX1B (guinea pig; Thomas Muller Lab), LHX1/5 (mouse;

(3) Embryonic motor neuron programming factors reactivate immature gene expression and suppress ALS pathologies in postnatal motor neuronsNature NeuroscienceOctober 1, 2025Emily R. Lowry, Tulsi Patel, Jonathon A. Costa, Elizabeth Chang, Shahroz Tariq, Hranush Melikyan, Ian Davis, Siaresh Aziz, Georgia Ntermentzaki, Francesco Lotti, et al.Millipore AB144P), ISL1 (1:10000, Thomas M. Jessell Laboratory, Columbia University or 1:5000, R&D Systems AF1837), LHX3 (1:5000, Thomas M. Jessell Laboratory, Columbia University), RFP (1:1000, Thermo Fisher 600-401-379),

(4) An organ-chip model of sporadic ALS using iPSC-derived spinal cord motor neurons and an integrated blood-brain-like barrierCell Stem CellJuly 3, 2025Deepti Lall, Michael J. Workman, Samuel Sances, Briana N. Ondatje, Shaughn Bell, George Lawless, Amanda Woodbury, Dylan West, Amanda Meyer, Andrea Matlock, et al.SMI32 (Biolegend, San Diego, CA, 801701, 1:1000), goat polyAb to Islet-1 (R&D Systems, Minneapolis, MN, AF1837, 1:500), rabbit Ki67 (abcam, Cambridge, MA, ab27619, 1:200). Secondary antibodies were mouse IgG conjugated

(5) Morphology and connectivity of retinal horizontal cells in two avian speciesFront Cell NeurosciMarch 4, 2025Anja Günther, Vaishnavi Balaji, Bo Leberecht, Julia J. Forst, Alexander Y. Rotov, Tobias Woldt, Dinora Abdulazhanova, Henrik Mouritsen, Karin Dedekprevious findings for the chicken retina (Araki and Kimura, 1991). The Islet-1 antibody (R&D Systems AF1837, RRID: AB2126324) has been validated through Western blot and immunocytochemistry in non-bird species.

(6) Modelling a pathological GSX2 variant that selectively alters DNA binding reveals hypomorphic mouse basal ganglia and hindbrain defectsDisease Models & MechanismsFebruary 1, 2025Laura Tweedie, Matthew R. Riccetti, Brittany Cain, Shenyue Qin, Joseph Salomone, Jordan A. Webb, Amy Riesenberg, Lisa A. Ehrman, Ronald R. Waclaw, Rhett A. Kovall, et al.2000), chicken anti-EGFP (1:1000; Aves, GFP-1010, RRID:AB_2307313), goat anti-Isl1 (1:500; R&D Systems, AF1837, RRID:AB_2126324), rabbit anti-ZFP503 (Nolz1) (1:1000; Sigma-Aldrich, HPA026848, RRID:AB_10610704),

(7) Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particlesNat CommunJanuary 4, 2025Thibaut Halegua, Valérie Risson, Julien Carras, Martin Rouyer, Laurent Coudert, Arnaud Jacquier, Laurent Schaeffer, Théophile Ohlmann, Philippe Emmanuel Mangeotbuffer. After three washes with PBS, cells were analysed. Antibody dilutions were: Islet1 R&D Systems AF1837 dilution factor 1/500, Olig2 Millipore AB9610, dilution factor 1/200, β3-tubuline Biolegend 801202,

(8) Netrin1 patterns the dorsal spinal cord through modulation of Bmp signalingCell ReportsNovember 26, 2024Sandy Alvarez, Sandeep Gupta, Yesica Mercado-Ayon, Kaitlyn Honeychurch, Cristian Rodriguez, Riki Kawaguchi, Samantha J. Butleral.76,N/A Mouse anti Isl1/2,Developmental Studies Hybridoma bank,Cat#39.4DS Goat anti Isl1/2,R&D Systems,"Cat#AF1837, RRID:AB_2126324" Rabbit anti Pax2,Invitrogen,Cat#71-6000 Guinea Pig anti Olig2,Gift from Bennett

(9) Identification of novel neuroprotectants against vincristine-induced neurotoxicity in iPSC-derived neuronsCellular and molecular life sciences : CMLSJuly 27, 2024Petrova V, Snavely AR, Splaine J, Zhen S, Singh B, Pandey R, Chen K, Cheng A, Hermawan C, Barrett LB, et al.donkey serum at 4 C overnight. The following primary antibodies were used: anti-Islet1/2 (R&D Systems, AF1837, 1:250), anti-choline acetyltransferase (Millipore, AB144P, 1:100), anti-HB9 (DSHB, 81.5C10, 1:25),

(10) Perivascular neurons instruct 3D vascular lattice formation via neurovascular contactCellMay 23, 2024Toma K, Zhao M, Zhang S, Wang F, Graham HK, Zou J, Modgil S, Shang WH, Tsai NY, Cai Z, et al.RRID:AB_2313614 mouse anti-Tuj1,Biolegend,Cat#801201; RRID:AB_2728521 goat anti-Islet1,R&D Systems,Cat#AF1837; RRID:AB_2126324 sheep anti-Pax6,R&D Systems,Cat#AF8150; RRID:AB_2827378 mouse anti-Hif1a,Novus Biologicals,Cat#NB100-449;

(11) Decoding the spatiotemporal regulation of transcription factors during human spinal cord developmentCell researchMarch 1, 2024Shi Y, Huang L, Dong H, Yang M, Ding W, Zhou X, Lu T, Liu Z, Zhou X, Wang M, et al.MASH1 (BD, 556604), PAX3 (DSHB, Pax3), CRABP1 (NOVUS, NB300-539), DRGX (NOVUS, NBP1-94059), ISL1 (R&D, AF1837), FOXP1 (Abcam, ab16645), LHX3 (DSHB, 67.4E12), LHX1 (Abcam, ab14554), CRYAB (NOVUS, NB100-2519), NTRK2

(12) Transcriptional control of motor pool formation and motor circuit connectivity by the LIM-HD protein Isl2eLifeOctober 23, 2023Lee Y, Yeo IS, Kim N, Lee DK, Kim KT, Yoon J, Yi J, Hong YB, Choi BO, Kosodo Y, et al.polyclonal),R&D Systems,Cat# AF5857,(1:1000) Antibody,Anti-Isl1 (goat polyclonal),R&D Systems,Cat# AF1837,(1:1000) Antibody,Anti-Isl2 (guinea pig polyclonal),PMID:14766174,Cat# AF1837,(1:8000) Antibody,Anti-Lhx1

(13) Large-scale differentiation of iPSC-derived motor neurons from ALS and control subjectsNeuronApril 1, 2023Michael J. Workman, Ryan G. Lim, Jie Wu, Aaron Frank, Loren Ornelas, Lindsay Panther, Erick Galvez, Daniel Perez, Imara Meepe, Susan Lei, et al.(1:1000),Biolegend,Cat#801701; RRID:AB_2564642 Goat anti-Human Islet-1/ISL1 (1:250),R&D Systems,Cat#AF1837; RRID:AB_2126324 Mouse anti-NKX6.1 (1:1000),DSHB,Cat#F55A10-s; RRID:AB_532378 Goat anti-NKX6.1,R&D Systems

(14) Tissue clearing of human iPSC-derived organ-chips enables high resolution imaging and analysisLab on a chipOctober 25, 2022Ondatje BN, Sances S, Workman MJ, Svendsen CN.for immunocytochemistry: mouse mAb to SMI32 (Biolegend, 801701, 1 : 1000), goat polyAb to Islet-1 (R&D Systems, AF1837, 1 : 500), rabbit Ki67 (abcam, ab27619, 1 : 200). Samples were then washed in 0.5% Triton-X solution

(15) The cardiopharyngeal mesoderm contributes to lymphatic vessel development in mouseeLifeOctober 5, 2022Maruyama K, Miyagawa-Tomita S, Haneda Y, Kida M, Matsuzaki F, Imanaka-Yoshida K, Kurihara H.BD Pharmingen, RRID: AB_394816, 1:100), Flk1 (555307, BD Pharmingen, RRID:AB_395720, 1:100), Isl1 (AF1837, R&D systems, RRID:AB_2126324, 1:250), Prox1 (11-002, AngioBio, RRID: AB_10013720, 1:200; AF2727, R&D Systems

(16) Moxifloxacin rescues SMA phenotypes in patient-derived cells and animal modelCellular and molecular life sciences : CMLSJuly 22, 2022Januel C, Menduti G, Mamchaoui K, Martinat C, Artero R, Konieczny P, Boido M.Model,Antibodies,Designation,Reference,Host,Provider,Dilution Motoneurons,Primary,Islet1,AF1837,Goat,R&D systems,1:500 Motoneurons,Primary,Tubulin β III (Tuj1),801201,Mouse,Biolegend,1:1000 Motoneurons,Primary,Tubulin

(17) In vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansionCell reportsJuly 19, 2022Gupta S, Kawaguchi R, Heinrichs E, Gallardo S, Castellanos S, Mandric I, Novitch BG, Butler SJ.RESOURCE,SOURCE,IDENTIFIER Antibodies,, ,, Goat polyclonal anti-Lhx2,Santa Cruz,Sc-19344; RRID:AB_2135660 Goat polyclonal anti-Isl1,R&D Systems,"AF1837, RRID:AB_2126324" Rabbit polyclonal anti-Pax2,Invitrogen,"71–6000, RRID:AB_2336046" Mouse monoclonal

(18) Rapid 3D-STORM imaging of diverse molecular targets in tissueCell Rep MethodsJuly 18, 2022Albrecht NE, Jiang D, Akhanov V, Hobson R, Speer CM, Robichaux MA, Samuel MA.AB_397879" GFAP,Sigma-Aldrich,"Cat# G3893, RRID: AB_477010" Iba1,Abcam,"Cat# ab5076, RRID: AB_2224402" Islet1,R&D System,"Cat# AF1837, RRID: AB_2126324" NG2,Abcam,"Cat# ab129051, RRID: AB_2877152" PKCα,Abcam,"Cat# ab31, RRID: AB_303507" PSD95,Abcam,"Cat#

(19) Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation modelCell reportsJune 21, 2022Ohgushi M, Taniyama N, Vandenbon A, Eiraku M.

(20) Transcription factor protein interactomes reveal genetic determinants in heart diseaseCellMarch 3, 2022Gonzalez-Teran B, Pittman M, Felix F, Thomas R, Richmond-Buccola D, Hüttenhain R, Choudhary K, Moroni E, Costa MW, Huang Y, et al.Kadonaga’s laboratory), NKX2-5 (Cat. Sc-8697 X, Santa Cruz), MEIS1 (Cat. Ab19867, Abcam), ISL1 (Cat. AF1837, R&D Systems). The specificity of the antibodies was validated in previous publications (Luna-Zurita

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12/29/2021Anti-Isl1 Antibody for Immunofluorescence José VieiraGood antibody to label Isl1+ cells.

Human Islet-1 Antibody

Islet-1 Antibody

Islet-1 Antibody

Islet-1 Antibody

Islet-1 Antibody

Islet-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (50)

Reviews

Islet-1 Antibody from R&D Systems, a Bio-Techne Brand

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