FABP5/E-FABP Antibody AF1476 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFABP5/E-FABP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF1476
NCBI Gene AliasesPA-FABP, Fabpe, KFABP, EFABP, E-FABP, mal1, Klbp, PAFABP
ApplicationsCo-Immunoprecipitation,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
TargetFABP5/E-FABP
ReactivityHuman,Mouse,Rat,Transgenic Mouse
IsotypeIgG
NCBI Full Gene Namefatty acid binding protein 5, epidermal, fatty acid binding protein 5
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse FABP5/E-FABP, Met1-Gln135, Accession # Q05816
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse FABP5/E-FABP in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

What applications has the FABP5/E-FABP antibody been used for in scientific research?

The FABP5/E-FABP antibody has been shown to work in various applications including Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry, as evidenced by its use in studies such as those by Bogdan et al. (2022) and Kretzschmar et al. (2014).

Can this antibody be used to study the role of FABP5 in pain signaling?

Yes, the FABP5/E-FABP antibody has been utilized to investigate the role of FABP5 in nociceptors, as demonstrated in research by Bogdan et al. (2022), which highlighted its involvement in modulating endocannabinoid signaling and TRPV1 sensitization in pain pathways.

Is the FABP5/E-FABP antibody effective in detecting FABP5 in skin tissues?

The antibody has been effectively used to detect FABP5 in skin tissues, as shown in studies like those by Yin et al. (2022) and Napoletano et al. (2019), where it was employed to analyze its expression in relation to skin inflammation and psoriasis.

What species does the FABP5/E-FABP antibody react with?

The FABP5/E-FABP antibody is reactive with mouse, human, and rat samples, making it suitable for studies across these species, as indicated by its validation in various research contexts.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Dietary Stearic Acid Accelerates Intestinal Tumorigenesis via Fatty Acid-binding Protein 5 Without Promoting ObesityCellular and Molecular Gastroenterology and HepatologyJanuary 28, 2026Kazuaki Nakata, Seiga Komiyama, Keisuke Sekine, Motoyoshi Nagai, Takuma Okawa, Wakana Ohashi, Kenta Nakano, Tadashi Okamura, Takuma Kozono, Nobuyuki Takemura, et al.this study included Ki67 (GeneTex, #GTX16667), Lysozyme C (Santa Cruz, #sc-27958), FABP5 (R&D System, #AF1476), Chr-A (Santa Cruz, #sc-393941), Mucin-2 (SantaCruz, #sc-15334), DCAMKL1 (Abgent, #Ap7219b), CD44 (Biolegend,

(2) An Olive Oil-Based High-Fat Diet Promotes Obesity-Driven Metastasis of Triple-Negative Breast CancerCancer researchDecember 15, 2025Avellino A, Jiang X, Lee M, Yu J, Liu S, Han X, Li J, Shilyansky J, Wang Z, Curry M, et al.

(3) FABP5 in skin macrophages mediates saturated fat-induced IL-1β signaling in psoriatic inflammationCell ReportsSeptember 23, 2025Jianyu Yu, Jiaqing Hao, Matthew S. Yorek, Xingshan Jiang, Anthony Avellino, Shanshan Liu, Xiaochun Han, Jonathan Shilyansky, Zhaohua Wang, Yuhang Wang, et al.Blue,Biolegend,Cat#505020; RRID: AB_2125094 Goat polyclonal anti-human/mouse FABP5,R&D Systems,Cat#AF1476; RRID: AB_2293656 Donkey polyclonal anti-rabbit IgG,Biolegend,Cat#406419; RRID: AB_2563453 Donkey

(4) Multi-omic identification of perineurial hyperplasia and lipid-associated nerve macrophages in human polyneuropathiesNat CommunAugust 23, 2025Michael Heming, Anna-Lena Börsch, Jolien Wolbert, Christian Thomas, Anne K. Mausberg, Fabian Szepanowski, Bianca Eggert, I-Na Lu, Julia Tietz, Finja Dienhart, et al.primary antibodies: rabbit anti-Osteopontin (1:100, Abcam, ab63856), goat anti-FABP5 (1:100, R&D Systems, AF1476), rabbit anti-Perilipin-2 (1:100, Novusbio, NBP2-48532), rabbit anti-CD36 (1:100, Abcam, ab124515),

(5) Astrocytic FABP5 mediates retrograde endocannabinoid transport at central synapsesiScienceMay 16, 2025Saida Oubraim, Mohammad Fauzan, Keith Studholme, Chris Gordon, Sherrye T. Glaser, Roh-Yu Shen, Iwao Ojima, Martin Kaczocha, Samir Haj-Dahmaneprimary antisera: rabbit anti-FABP5 (BioVendor R&D, RD181060100), goat anti-FABP5 (R&D Systems Inc, #AF1476), mouse anti-NeuN (Millipore, MAB377), rabbit anti-NeuN (Abcam, #AB104225), and mouse anti-s100β (Sigma,

(6) Expression profiles of FABP4 and FABP5 in breast cancer: clinical implications and perspectivesDiscov OncolMarch 19, 2025Xingshan Jiang, Yiqin Xiong, Jianyu Yu, Anthony Avellino, Shanshan Liu, Xiaochun Han, Zhaohua Wang, Jonathan S. Shilyansky, Melissa A. Curry, Jiaqing Hao, et al.4 °C with primary antibodies, including anti-FABP4 (R&D Systems, AF3150), anti-FABP5 (R&D Systems, AF1476), anti-human vWF (Novus Biologicals, NB600-586) or anti-CD163 (Abcam, ab182422). Depending on the primary

(7) Transgelin 2 guards T cell lipid metabolism and antitumour functionNatureNovember 28, 2024Sung-Min Hwang, Deepika Awasthi, Jieun Jeong, Tito A. Sandoval, Chang-Suk Chae, Yusibeska Ramos, Chen Tan, Matías Marin Marin Falco, Camilla Salvagno, Alexander Emmanuelli, et al.1:200; BD Biosciences), CD45.1 (A20, 1:200), CD45.2 (104, 1:200; BD Biosciences), anti-FABP5 (primary; AF1476, 1:100; R&D Systems, secondary; Donkey anti-Goat IgG (H+L) Alexa Fluor™ Plus 647; A32849, 1:200; Invitrogen),

(8) Biofunctionalized gelatin hydrogels support development and maturation of iPSC-derived cortical organoidsCell reportsNovember 26, 2024Kjar A, Haschert MR, Zepeda JC, Simmons AJ, Yates A, Chavarria D, Fernandez M, Robertson G, Abdulrahman AM, Kim H, et al.1:50,Sigma-Aldrich,Cat# HPA030180; RRID: AB_10603770 Goat polyclonal anti-FABP5 1:250,R&D Systems,Cat#AF1476; RRID: AB_2293656 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(9) Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometrySTAR protocolsJune 21, 2024Yu J, Hao J, Yorek MS, Han X, Avellino A, Jiang X, Liu S, Wang J, Li B.(1:300),Proteintech,"Cat#10590-1-AP, RRID: AB_2134306" Goat polyclonal anti-human/mouse FABP5 (1:300),R&D Systems,Cat#AF1476; RRID: AB_2293656 "Donkey polyclonal anti-rabbit IgG-BV510 (1:2,000)",BioLegend,Cat#406419; RRID: AB_2563453 "Donkey

(10) Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signalingCell reportsApril 23, 2024Zhong D, Chen J, Qiao R, Song C, Hao C, Zou Y, Bai M, Su W, Yang B, Sun D, et al.

(11) Fatty acid binding protein 5 suppression attenuates obesity-induced hepatocellular carcinoma by promoting ferroptosis and intratumoral immune rewiringNature metabolismApril 1, 2024Sun J, Esplugues E, Bort A, Cardelo MP, Ruz-Maldonado I, Fernández-Tussy P, Wong C, Wang H, Ojima I, Kaczocha M, et al.30 min. Liver sections were incubated overnight at 4 °C with primary FABP5 antibodies (R&D Systems; AF1476). The following day, sections were incubated for 30 min at room temperature with biotinylated rabbit

(12) Transgelin 2 guards T cell lipid metabolic programming and anti-tumor functionResearch squareDecember 14, 2023Hwang SM, Awasthi D, Jeong J, Sandoval TA, Chae CS, Ramos Y, Tan C, Falco MM, McBain IT, Mishra B, et al.

(13) Epidermal Fatty Acid‒Binding Protein Mediates Depilatory-Induced Acute Skin InflammationThe Journal of investigative dermatologyJuly 1, 2022Yin D, Hao J, Jin R, Yi Y, Bodduluri SR, Hua Y, Anand A, Deng Y, Haribabu B, Egilmez NK, et al.were stained with anti-Ly6G (Cat# 127607, Biolegend), anti-Gr-1 (Cat#,108442 Biolegend), E-FABP (Cat# AF1476, R&D system), cPLA24E (Cat # 18088–1-AP, Thermo) for 30 mins on ice, then stained with 0.2μM DAPI (Cat#

(14) FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory painScientific reportsJune 2, 2022Bogdan DM, Studholme K, DiBua A, Gordon C, Kanjiya MP, Yu M, Puopolo M, Kaczocha M.Antibody,Dilution,Supplier,Cat #,RRID FABP5,1:500,BioVendor,RD181060100,AB_344491 FABP5,1:600,R&D Systems,AF1476,AB_2293656 TRPV1,1:600,EMD Millipore,AB5566,AB_91901 CB1,1:500,Abcam,Ab172970,AB_2692328 TrkA,1:200,R&D Systems

(15) Destructing biofilms by cationic dextran through phase transitionCarbohydrate polymersMarch 1, 2022Li Y, Wang S, Xing Z, Niu Y, Liao Z, Lu Y, Qiu J, Zhang J, Wang C, Dong L.

(16) Stroke induces disease-specific myeloid cells in the brain parenchyma and piaNature communicationsFebruary 17, 2022Beuker C, Schafflick D, Strecker JK, Heming M, Li X, Wolbert J, Schmidt-Pogoda A, Thomas C, Kuhlmann T, Aranda-Pardos I, et al.Rabbit-anti-MS2/Adam8 (1:100, clone EPR22688-44, Abcam, ab255608), Goat-anti-FABP5 (1:100, R&D Systems, AF1476), Chicken-anti-MAP2 (1:500, Abcam, ab5392), BODIPY 493/503 (1:1000, Molecular Probes, D3922) and Rabbit-anti-Perilipin-2

(17) Fatty Acid-Binding Protein 5 Modulates Brain Endocannabinoid Tone and Retrograde Signaling in the StriatumFrontiers in cellular neuroscienceJanuary 1, 2022Fauzan M, Oubraim S, Yu M, Glaser ST, Kaczocha M, Haj-Dahmane S.sections (30 μm) were incubated with the following primary antisera: goat anti-FABP5 (R&D Systems Inc, #AF1476), mouse anti-NeuN (Millipore, MAB377), and mouse anti-s100β (Sigma, #S2532). Secondary antibodies used

(18) Mutant Lef1 controls Gata6 in sebaceous gland development and cancerThe EMBO journalMay 2, 2019Oulès B, Rognoni E, Hoste E, Goss G, Fiehler R, Natsuga K, Quist S, Mentink R, Donati G, Watt FM.Walko et al, 2017). Primary antibodies were used at the indicated dilutions: Fabp5 (1:100, R&D Systems AF1476); Krt15 (1:1,000, LHK‐15 clone, Abcam ab80522); Lef1 (1:100–500, C12A5 clone, Cell Signaling 2230 and

(19) Arid1a Loss Drives Nonalcoholic Steatohepatitis in Mice Through Epigenetic Dysregulation of Hepatic Lipogenesis and Fatty Acid OxidationHepatology (Baltimore, Md.)May 1, 2019Moore A, Wu L, Chuang JC, Sun X, Luo X, Gopal P, Li L, Celen C, Zimmer M, Zhu H.(SCBT, sc-13551), anti-FAS mouse antibody (SCBT, sc-48357), anti-FABP5 goat antibody (R&D Systems, AF1476), Anti-rabbit IgG, HRP-linked Antibody (Cell signaling, #7074), Anti-mouse IgG, HRP-linked Antibody

(20) Intersections between Regulated Cell Death and AutophagyTrends in cell biologyApril 1, 2019Napoletano F, Baron O, Vandenabeele P, Mollereau B, Fanto M.Walko et al, 2017). Primary antibodies were used at the indicated dilutions: Fabp5 (1:100, R&D Systems AF1476); Krt15 (1:1,000, LHK-15 clone, Abcam ab80522); Lef1 (1:100–500, C12A5 clone, Cell Signaling 2230 and

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