Ephrin-B2 Antibody AF496 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEphrin-B2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF496
ApplicationsProximity Ligation Assay (PLA),Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,CyTOF-ready,Functional Assay
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetEphrin-B2
ReactivityHuman,Mouse,Rat,Transgenic Mouse,Frog - Xenopus (African Clawed Frog)
HostGoat
Quantity100 ug (also 25 ug)
IsotypeIgG
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2, Arg27-Ala227, Accession # AAA82934
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameephrin B2
NCBI Gene AliasesEPLG5, HTKL, Htk-L, LERK5, ephrin-B2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this Ephrin-B2 antibody be used for studying memory formation in the amygdala?

Yes, the Ephrin-B2 antibody has been utilized in research demonstrating its role in long-term fear memory formation in the basolateral amygdala of mice. Deleting Ephrin-B2 from excitatory neurons and astrocytes in this region impairs long-term memory, indicating its significance in synaptic transmission and memory processes ().

Is the Ephrin-B2 antibody effective for immunohistochemistry in cardiac studies?

The Ephrin-B2 antibody has been shown to be effective in immunohistochemical applications, particularly in studies investigating cardiac lymphangiogenesis and post-myocardial infarction inflammation. It plays a crucial role in promoting cardiac lymphangiogenesis and improving cardiac function after ischemic injury ().

What species can this antibody be used on for research purposes?

The Ephrin-B2 antibody is reactive to multiple species, including human, mouse, rat, and Xenopus (amphibian), making it versatile for various experimental models in neuroscience and cardiovascular research.

Has this antibody been cited in studies related to axon guidance?

Yes, the Ephrin-B2 antibody has been cited in studies examining its expression patterns in the developing nervous system, particularly in relation to axon guidance during embryonic development. It has been used to investigate the alignment of Ephrin-B2 with developing neural structures, indicating its importance in neurodevelopmental processes ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Identification of New Ciliary Signaling Pathways in the Brain and Insights into Neurological DisordersJ NeurosciAugust 13, 2025Abdelhalim Loukil, Emma Ebright, Karama Hamdi, Elizabeth Menzel, Akiyoshi Uezu, Yudong Gao, Scott H. Soderling, Sarah C. GoetzInvitrogen, PA5-35382); anti-Ephrin-B1 (IF, 1:50; Bio-Techne, AF473); anti-Ephrin-B2 (IF, 1:50; Bio-Techne, AF496); anti-EphA4 (IF, 1:50; WB, 1:2,000; Proteintech, 21875-1-AP); anti-CSNK1G1 (IF, 1:50; OriGene, TA806333S);

(2) Identification of New Ciliary Signaling Pathways in the Brain and Insights into Neurological DisordersJ NeurosciJuly 7, 2025Abdelhalim Loukil, Emma Ebright, Karama Hamdi, Elizabeth Menzel, Akiyoshi Uezu, Yudong Gao, Scott H. Soderling, Sarah C. GoetzInvitrogen, PA5-35382); anti-Ephrin-B1 (IF, 1:50; Bio-Techne, AF473); anti-Ephrin-B2 (IF, 1:50; Bio-Techne, AF496); anti-EphA4 (IF, 1:50; WB, 1:2,000; Proteintech, 21875-1-AP); anti-CSNK1G1 (IF, 1:50; OriGene, TA806333S);

(3) Arterial-Lymphatic-Like Endothelial Cells Appear in Hereditary Hemorrhagic Telangiectasia 2 and Contribute to Vascular Leakage and Arteriovenous MalformationsCirculationFebruary 4, 2025Yang Y, Wu X, Zhao Y, Zhang D, Zhang L, Cai X, Ji J, Jing Z, Boström KI, Yao Y.against LYVE1 (Thermo Fisher Scientific, catalog No. 12-0443-82) and Ephrin B2 (R&D Systems, catalog No. AF496). Nonspecific fluorochrome- and isotype-matched IgGs (BD Pharmingen) served as controls. Immunoblotting

(4) Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCCOncogeneFebruary 1, 2025Abdelazeem KNM, Nguyen D, Corbo S, Darragh LB, Matsumoto MW, Van Court B, Neupert B, Yu J, Olimpo NA, Osborne DG, et al.

(5) Long-range organization of intestinal 2D-crypts using exogenous Wnt3a micropatterningNature communicationsJanuary 3, 2025Larrañaga E, Marin-Riera M, Abad-Lázaro A, Bartolomé-Català D, Otero A, Fernández-Majada V, Batlle E, Sharpe J, Ojosnegros S, Comelles J, et al.

(6) EphrinB2-mediated CDK5/ISL1 pathway enhances cardiac lymphangiogenesis and alleviates ischemic injury by resolving post-MI inflammationSignal transduction and targeted therapyNovember 18, 2024Bai Y, Chen L, Guo F, Zhang J, Hu J, Tao X, Lu Q, Li W, Chen X, Gong T, et al.overnight at 4 °C in a humidified chamber. The antibodies utilized were as follows: EphrinB2 (10 μg/ml;# AF496, R&D system), VEGFR3/FLT4 (2 μg/ml; #AF743, R&D system), LYVE-1 (1:100; #67538, Cell Signal Technology),

(7) EphrinB2 in excitatory neurons and astrocytes in the basolateral amygdala controls long-term fear memory formationCommunications biologySeptember 17, 2024Agarwal K, Farhat A, Lamprecht R.Triton and 3% BSA. Sections were then incubated overnight at 4 °C with the anti-ephrinB2 (R&D Systems, AF496-SP1:100), anti-EAAT1 (Cell Signaling Technology, #5684; 1:100), anti-DCX (Cell Signaling Technology,

(8) Identification of new ciliary signaling pathways in the brain and insights into neurological disordersbioRxiv : the preprint server for biologyDecember 21, 2023Loukil A, Ebright E, Uezu A, Gao Y, Soderling SH, Goetz SC.Invitrogen, PA5-35382); Anti-Ephrin-B1 (IF: 1:50, Bio-Techne, AF473); Anti-Ephrin-B2 (IF: 1:50, Bio-Techne, AF496); Anti-EphB2 (IF: 1:50, Bio-Techne, AF467); Anti-EphA4 (IF: 1:50, Proteintech, 21875-1-AP); Anti-CSNK1G1

(9) SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformationsThe Journal of clinical investigationSeptember 15, 2023Banerjee K, Lin Y, Gahn J, Cordero J, Gupta P, Mohamed I, Graupera M, Dobreva G, Schwartz MA, Ola R.no. CX40-A; 1:200), anti-PH3 (Sigma-Aldrich no. 06-570; 1:200), and anti-Ephrinb2 (R&D Systems no. AF496; 1:100). For Western blotting, the following antibodies were used: anti-pAkt (Cell Signaling no. 4060;

(10) EphrinB2 knockdown in cervical spinal cord preserves diaphragm innervation in a mutant SOD1 mouse model of ALSbioRxiv : the preprint server for biologyAugust 11, 2023Urban MW, Charsar BA, Heinsinger NM, Markandaiah SS, Sprimont L, Zhou W, Brown EV, Henderson NT, Thomas SJ, Ghosh B, et al.

(11) Actin cable formation and epidermis-dermis positional relationship during complete skin regenerationScientific reportsSeptember 23, 2022Takaya K, Okabe K, Ishigami A, Imbe Y, Kanazawa H, Sakai S, Aramaki-Hattori N, Kishi K.antibodies: rat anti-E-cadherin (SAB4200684; Sigma-Aldrich, St. Louis, MO, USA; 1:200), goat anti-ephrin-B1 (AF496; R&D Systems, Minneapolis, MN, USA; 1:200), and rabbit anti-Pdlim5 (10530-1-AP; Proteintech, Rosemont,

(12) Histochemical examination of blood vessels in murine femora with intermittent PTH administrationJournal of oral biosciencesSeptember 1, 2022Maruoka H, Zhao S, Yoshino H, Abe M, Yamamoto T, Hongo H, Haraguchi-Kitakamae M, Nasoori A, Ishizu H, Nakajima Y, et al.

(13) Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra virusesCellJuly 7, 2022Ang LT, Nguyen AT, Liu KJ, Chen A, Xiong X, Curtis M, Martin RM, Raftry BC, Ng CY, Vogel U, et al.gene),Abcam,ab92513 (1:500) ,, Mouse Anti-Human CD144 (VE-Cad),BD Bioscience,555661 (1:500) Anti-EFNB2,R&D Systems,AF496 (1:5) APC Donkey Anti-Goat,R&D Systems,F0108 (1:10) Alexa Fluor 568 Donkey Anti-rabbit,Thermo Fisher

(14) Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injuryExperimental eye researchJune 1, 2022Guaiquil VH, Xiao C, Lara D, Dimailig G, Zhou Q.anti goat TRITC,1:400,Jackson Immunoresearch 705-025-147 EphrinB2,Goat,1:200,"mchc, tg",R&D Systems AF496,Donkey anti goat Alexa green 488Donkey anti goat TRITC,1:4001:400,ThermoFisherA11055Jackson Immunoresearch

(15) Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation cultureRegenerative therapyMarch 1, 2022Yamasaki S, Kuwahara A, Kishino A, Kimura T, Takahashi M, Mandai M.Biologicals,X1180P,AB_2314191,1:500 Collagen-IV,Rabbit,Abcam,ab6311,AB_305414,1:500 Crx,Rabbit,Takara Bio Inc.,M231,,1:500 EphrinB2,Goat,R&D Systems,AF496,AB_2095679,1:500 Laminin,Rabbit,Abcam,ab-11575,AB_298179,1:500 Mitf,Mouse,Exalpha Biologicals,X2398M,,1:500 Nrl,Goat,R&D Systems

(16) EphrinB2-RhoA upregulation attenuates sympathetic hyperinnervation and decreases the incidence of ventricular arrhythmia after myocardial infarctionJournal of cardiologyMarch 1, 2022Wang Y, Shao C, Qi L, Tan J, Zhao Y, Xue M, Li X, Cheng W, Li X, Yin J, et al.

(17) Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4(+) endothelial cellsCell metabolismSeptember 7, 2021Fan Z, Turiel G, Ardicoglu R, Ghobrial M, Masschelein E, Kocijan T, Zhang J, Tan G, Fitzgerald G, Gorski T, et al.1:500), anti-α smooth muscle actin Cy3 conjugated (αSMA) (1:500, C6198, Sigma-Aldrich), anti-Ephrin-B2 (AF496, R&D Systems,1:200), anti- Carbonic Anhydrase IV (Car4) (PA5-47312 Thermo Fischer Scientific, 1:250),

(18) The Value of EphB2 Receptor and Cognate Ephrin Ligands in Prognostic and Predictive Assessments of Human Breast CancerInternational journal of molecular sciencesJuly 28, 2021Ebrahim AS, Hailat Z, Bandyopadhyay S, Neill D, Kandouz M.Inc. #AM11063SU-N) diluted to 1/100, anti-ephrinB1 (R&D Systems #AF473), anti-ephrin-B2 (R&D Systems #AF496), anti-ephrin-B3 (R&D Systems #AF395), and anti-Ephrin-A5 antibody (R&D Systems #AF3743) diluted to

(19) Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous FistulaeThe Journal of surgical researchJune 1, 2021Wang T, Liu J, Liu H, Lee SR, Gonzalez L, Gorecka J, Shu C, Dardik A.Target Antigen,Vendor or Source,Identifier,Working Concentration EphrinB2,R&D Systems,"Cat# AF496,RRID: AB_2095679",IF 1:100 EphrinB2 (HRP),Abcam,"Cat# ab201512,RRID: AB_2810831",WB 1:8000 eNOS,BD biosciences

(20) Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndromeYearbook of Paediatric EndocrinologyOctober 23, 2020Li M et al.BD ARTICLE Biosciences, 555289; Phalloidin, Alexa-Fluor-647, 1/500, Invitrogen, A22287; antiephrin-B2, 1/100, R&D Systems AF496), diluted in blocking buffer (2% FBS diluted in PBS) were applied overnight at 4 °C. After washing,

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