CCL5/RANTES Antibody AF478 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCCL5/RANTES Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF478
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunocytochemistry
Conjugate/TagUnconjugated
IsotypeIgG
TargetCCL5/RANTES
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Rat
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse CCL5/RANTES
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameC-C motif chemokine ligand 5
NCBI Gene AliasesD17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CCL5/RANTES in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) DMXAA accelerates orthodontic tooth movement via macrophage-mediated Rab13-enriched extracellular vesicle release and chemokine secretionProgress in OrthodonticsJune 29, 2026Yan Wang, Kejia Zhang, Xueyu Sun, Hao Xu, Heng Gu, Zhichun Jin, Linlin Zhu, Hanwen Zhang, Bin Yansupernatants from DMXAA-treated BMDM with or without containing antibodies of CCL5 (concentration: 0.2 µg/mL, #AF478, R&D systems) and CXCL10 (concentration: 0.5 µg/mL, #AF-466-NA, R&D systems) were incubated osteoclasts

(2) Loss of SOCS1 in Donor T Cells Exacerbates Intestinal GVHD by Driving a Chemokine‐Dependent Pro‐Inflammatory Immune MicroenvironmentAdvanced ScienceMarch 1, 2026Zhigui Wu, Bixia Wang, Xinya Jiang, Fangqing Zhang, Qianqian Huang, Xinyi Wu, Shuang Fan, Qi Zhang, Jingrui Zhou, Jianing Tang, et al.mice. Recipient mice were treated with anti‐CCL5 neutralizing antibodies (10 µg/mouse; R&D Systems, AF478) or isotype control antibodies. Antibodies were diluted in PBS and administered i.v. on days 0, 3, 5,

(3) Dendritic cells regulate the innate-adaptive balance in lymph nodes for optimal host defenseCellJanuary 22, 2026Jessica Y. Huang, Michael Y. Gernerpolyclonal anti-CCL4/MIP-1 beta,R&D Systems,CAT# AF451 Goat polyclonal anti-CCL5/RANTES,R&D Systems,CAT# AF478 Goat polyclonal anti-CCL21/6Ckine,R&D Systems,CAT# AF457 Goat polyclonal anti-CCL22/MDC,R&D Systems,CAT#

(4) Tumors hijack macrophages for iron supply to promote bone metastasis and anemiaCellOctober 30, 2025Yujiao Han, Hirak Sarkar, Zhan Xu, Sereno Lopez-Darwin, Yong Wei, Xiang Hang, Fengshuo Liu, Kimberley Tran, Wei Wang, Jennifer M. Miller, et al.media. To assess the effect of blocking migration, neutralizing antibodies against CCL5 (R&D Systems, #AF478, 500 ng/ml) and IL34 (R&D Systems, # MAB5195, 500 ng/ml) or small-molecule inhibitors targeting CSF1R

(5) Impaired macroautophagy in oligodendrocyte precursor cells suppresses neuronal plasticity via a senescence-associated signalingScience AdvancesSeptember 26, 2025Hong Chen, Yan-Yun Sun, Qi-Fa Li, Yu-Tong Du, Na-Na Hu, Ao-Ran Sui, Xiao-Qing Luo, Xin Huang, Chao Zhu, Gang Yang, et al.anti–CCL3/MIP-1 alpha (R&D Systems, AF-450-NA, RRID: AB_354492), goat anti-CCL5/RANTES (R&D Systems, AF478, RRID: AB_355385), normal goat immunoglobulin G (IgG) control (R&D Systems, AB-108-C, RRID: AB_354267),

(6) Astrocyte-derived CCL5-mediated CCR5(+) neutrophil infiltration drives depression pathogenesisScience advancesMay 23, 2025Yao H, Jiang SY, Jiao YY, Zhou ZY, Zhu Z, Wang C, Zhang KZ, Ma TF, Hu G, Du RH, et al.1:1000, Millipore, USA), anti-Ly6G (ab25377, 1:400, Abcam), anti-CCR5 (ab7346, 1:500, Abcam), anti-CCL5 (AF478, 1:500, R&D Systems, USA), anti-NeuN (24307S, 1:1000, Cell Signaling Technology, USA), anti-IBA1 (ab5076,

(7) Lactobacillus intestinalis facilitates tumor-derived CCL5 to recruit dendritic cell and suppress colorectal tumorigenesisGut MicrobesJanuary 8, 2025Yong Sun, Qiwen Wang, Yao Jiang, Jiamin He, Dingjiacheng Jia, Man Luo, Wentao Shen, Qingyi Wang, Yadong Qi, Yifeng Lin, et al.room temperature, permeabilized with 0.5% Triton X-100, and stained with anti-CCL5 antibody (1:100, #AF478, R&D systems, USA) overnight at 4°C. DyLight 488 conjugated rabbit anti-goat antibody (1:200, #FDO161,

(8) A sexually dimorphic hepatic cycle of periportal VLDL generation and subsequent pericentral VLDLR-mediated re-uptakeNature CommunicationsSeptember 28, 2024Tomaz Martini, Cédric Gobet, Andrea Salati, Jérôme Blanc, Aart Mookhoek, Michael Reinehr, Graham Knott, Jessica Sordet-Dessimoz, Felix Naef1: 300), mouse anti-GS/GLUL (Santa Cruz sc-74430, clone E-4, 1: 100) and goat anti-E-CAD (R&D systems AF478, 1: 200). After washes with 1x PBS, secondary antibodies were applied simultaneously for 40 min at RT:

(9) Protein phosphatase 2Cm-regulated branched-chain amino acid catabolic defect in dorsal root ganglion neurons drives pain sensitizationActa neuropathologica communicationsSeptember 10, 2024Lian N, Li F, Zhou C, Yin Y, Kang Y, Luo K, Lui S, Li T, Lu P.

(10) Rapid functional impairment of natural killer cells following tumor entry limits anti-tumor immunityNature communicationsJanuary 24, 2024Dean I, Lee CYC, Tuong ZK, Li Z, Tibbitt CA, Willis C, Gaspal F, Kennedy BC, Matei-Rascu V, Fiancette R, et al.

(11) Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitorsOncogeneMarch 1, 2023Vorontsova A, Cooper TJ, Haj-Shomaly J, Benguigui M, Levin S, Manobla B, Menachem R, Timaner M, Raviv Z, Shaked Y.(CM). CM (1 ml) was depleted of CCL5 or CXCL16 by overnight incubation at 4 °C with 1 µg anti-CCL5 (AF478, R&D Systems) or anti-CXCL16 (AF503, R&D Systems) antibodies, respectively. The CM was then incubated

(12) RGMa regulates CCL5 expression via the BMP receptor in experimental autoimmune encephalomyelitis mice and endothelial cellsMolecular medicine reportsMarch 1, 2022Tang S, Su B, Tao T, Yan W, Zhang R, Qin X, Feng J.room temperature. Subsequently, the sections were incubated with CCL5 primary antibodies (cat. no. AF478; 1:200 dilution; R&D Systems, Inc.) at 4°C overnight. Sections were then washed and incubated with tetramethylrhodamine

(13) ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activationCellular and molecular life sciences : CMLSFebruary 26, 2022Kim HJ, Kim WJ, Shin HR, Yoon HI, Moon JI, Lee E, Lim JM, Cho YD, Lee MH, Kim HG, et al.USA), mouse CCL2 antibody (AB-479-NA; R&D Systems, Inc, Minneapolis, MN, USA), mouse CCL5 antibody (AF478; R&D Systems), mouse CCL7 antibody (AF-456-NA; R&D Systems), and normal goat IgG control (AB-108-C;

(14) CCR5 maintains macrophages in the bone marrow and drives hematopoietic failure in a mouse model of severe aplastic anemiaLeukemiaNovember 1, 2021Seyfried AN, McCabe A, Smith JNP, Calvi LM, MacNamara KC.software and analyzed using FlowJo software (TreeStar, Ashland, OR). CCL5 neutralization Anti-CCL5 (AF478; 150 μg; R&D Systems) or isotype control antibody was diluted in PBS and administered to mice via intravenous injection

(15) Boosting immune surveillance by low-dose PI3K inhibitor facilitates early intervention of breast cancerAmerican journal of cancer researchJanuary 1, 2021Wang J, Zhang Y, Xiao Y, Yuan X, Li P, Wang X, Duan Y, Seewaldt VL, Yu D.

(16) Cytotoxic T cells swarm by homotypic chemokine signallingeLifeOctober 13, 2020Galeano Niño JL, Pageon SV, Tay SS, Colakoglu F, Kempe D, Hywood J, Mazalo JK, Cremasco J, Govendir MA, Dagley LF, et al.MAB21651-100 RRID:AB_2801441,(10 µg/ml) Antibody,Anti-CCL5 (polyclonal goat IgG),R & D Systems,Cat. #: AF478 RRID:AB_10080077,(1 µg/ml) Antibody,Anti-CCL9/10 (monoclonal rat IgG1),R & D Systems,Cat. #: MAB463

(17) The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injuryPloS oneJanuary 1, 2020Yagura K, Ohtaki H, Tsumuraya T, Sato A, Miyamoto K, Kawada N, Suzuki K, Nakamura M, Kanzaki K, Dohi K, et al.(25H14L17),Rabbit,"Thermo Fisher Scientific (Waltham, MA)",701030,100 CCL5,Goat,"R&D systems (Minneapolis, MN)",AF478,300 CCR2,Goat,"Novus Biologicals (Littleton, Co)",NB100-701,300 CCR2,Goat,"GeneTex (Irvine, CA)",GTX45788,300 CCR5,Rabbit,"Bioss

(18) Translational repression of Ccl5 and Cxcl10 by 4E-BP1 and 4E-BP2 restrains the ability of mouse macrophages to induce migration of activated T cellsEuropean journal of immunologyAugust 1, 2019William M, Leroux LP, Chaparro V, Graber TE, Alain T, Jaramillo M.

(19) Deletion of C-C Motif Chemokine Ligand 5 Worsens Invariant Natural Killer T-Cell-Mediated Hepatitis via Compensatory Up-regulation of CXCR2-Related Chemokine ActivityCellular and molecular gastroenterology and hepatologyJanuary 1, 2019Chen L, Gu J, Qian Y, Li M, Qian Y, Xu M, Li J, Wen Y, Xia L, Li J, et al.until there were no salt crystals. The cells were stained by mouse CCL5/RANTES antibody (cat number: AF478; R&D System) and visualized by Alexa Fluor 488 AffiniPure Donkey anti-goat immunoglobulin G (H+L) Alexa

(20) NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune ControlCellFebruary 22, 2018Böttcher JP, Bonavita E, Chakravarty P, Blees H, Cabeza-Cabrerizo M, Sammicheli S, Rogers NC, Sahai E, Zelenay S, Reis e Sousa C.the time of tumor transplantation, followed by a second injection two days later (R&D Systems; CCL5: AF478 and MAB478; XCL1: AF486, MAB486; Isotype controls: AB-108-C, MAB006). Processing of tumor tissue Unless

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CCL5/RANTES Antibody

CCL5/RANTES Antibody

CCL5/RANTES Antibody

CCL5/RANTES Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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CCL5/RANTES Antibody from R&D Systems, a Bio-Techne Brand

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