KAP3 Antibody PA5-76170 from Thermo Fisher Scientific

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Western blot was performed using Anti-KAP3 Polyclonal Antibody (Product # PA5-76170) and a 80 kDa band corresponding to KAP3 was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), NIH/3T3 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), DU 145 (Lane 5) and tissue extract (30 µg lysate) of Mouse Brain (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band at ~32 kDa was also observed in some of the samples tested.
ICC/IF

Immunofluorescence analysis of KAP3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KAP3 Polyclonal Antibody (Product # PA5-76170) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in cytosol and nucleus. Panel e represents cells with no primary antibody to assess background. The images were captured at 60X magnification..

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Thermo Fisher Scientific for
KAP3 Antibody

Thermo Fisher Scientific's KAP3 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, Immunoprecipitation, and Western Blot. The KAP3 Antibody was generated using KIFAP3 as the antigen and it reacts with Homo Sapiens, Human, Mouse, and Rat.

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KAP3 Antibody from Thermo Fisher Scientific

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KAP3 Antibody from Thermo Fisher Scientific

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