Fig 1: 4-AP promotes division reinnervation and enhanced PGP-9.5 expression. (a) Representative image of co-immunostaining for Ki-67+ cells (red) and NF-H+ cells (yellow) surrounding de novo hair follicles in the healed wound at day 14 and dashed line denotes the epidermal/dermal border. Scale bars = 20 μm. (b,c) Ki67+ and NF-H+ cells were quantified. Data represents 20 images from 5 different mouse wounds and are shown as mean ± SEM, n = 5 animals per group, statistical significance indicated by asterisks (O = saline mouse data points, ∆ = 4-AP treated mouse data points,* = p between 0.01 and 0.05, and *** = p between 0.001 and 0.0002 vs. saline). (d) Representative images of PGP-9.5 protein expression by immunofluorescence staining of healed wounds. PGP-9.5 (red) and nuclear stain DAPI (blue) and the dashed line denotes the epidermal/dermal border. Scale bars = 20 μm. (e) Quantification (integrated density) of PGP-9.5 protein expression. Data represents 20 images from 5 different mouse wounds and are shown as mean ± SEM, n = 5 animals per group, statistical significance indicated by asterisks (O = saline mouse data points, ∆ = 4-AP treated mouse data points, ** = p between 0.01 and 0.001 vs. saline).