Novus Biologicals
NF-H Antibody
NB300-217
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Our research mainly focuses on neurodegenerative processes using animal models. The specific goal of this experiment was to evaluate axonal integrity in the course of disease progression. This antibody clearly highlighted axons of the optic nerve.
Immunofluorescence
Feline
Diluted at 1:5000, overnight at 4°C
10% normal donkey serum + 0.5% triton X + 0.1M PBS
Alexa 568 conjugated anti-chicken IgY 1:500, 1 hr at RT
N/A
By an epifluorescent microscope with an appropriate filter
This experiment demonstrated robust and specific signals in axons of the optic nerve (red). DAPI (blue).
Robust and specific staining
None
Highly recommended for axonal staining in central nerve system.