Fig 1: SLC5A12 Blockade Promotes the Egress of CD4+ T Cell from the Inflamed Tissue(A) Organ culture schematic describing the analysis performed to assess the egress of mononuclear cells (MCs) from the inflamed tissue.(B) Analysis of MCs (CD4+, CD8+, CD19+, and CD14+) egress from tonsil tissues (n = 3, each in duplicate) cultured with sodium lactate (10 mM) and/or SLC5A12 Ab, or left untreated. Untreated MCs (CN, dotted line) set to 100.(C and D) Representative flow cytometry plots (D) and quantification (E) of egressed CD4+ T cells from RA synovial tissues (n = 3) cultured with sodium lactate (10 mM) and/or SLC5A12 Ab, 3C7 mAb, 10E11 mAb, or left untreated. Untreated MCs (CN, dotted line) set to 100.Two-tailed Student’s t test. Data expressed as mean ± SEM. *p = 0.05; **p = 0.01; ***p = 0.001. See also Figure S5.