IL-17/IL-17A Antibody AF-317-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-17/IL-17A Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF-317-NA
NCBI Gene AliasesIL-17A, ILA17, CTLA-8, CTLA8, IL17, IL-17
ApplicationsELISA Development,Tissue Culture,Mass Cytometry,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready,Cell Culture,Imaging Mass Cytometry,Dual RNAscope ISH-IHC Compatible,ELISA Detection
Conjugate/TagUnconjugated
TargetIL-17/IL-17A
IsotypeIgG
ReactivityCanine,Human,Mouse
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
Quantity100 ug (also 25 ug)
NCBI Full Gene Nameinterleukin 17A
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human IL-17, Ile20-Ala155, Accession # Q16552
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17, approximately 10% cross-reactivity with recombinant human IL-17F and less than 5% cross-reactivity with recombinant mouse IL-17 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunohistochemistry in human tissues?

Yes, the IL-17/IL-17A antibody has been utilized in immunohistochemistry for human tissues, as demonstrated in studies such as and . It has been shown to effectively detect IL-17A in various pathological contexts.

What species does this antibody react with, and is there any cross-reactivity?

The IL-17/IL-17A antibody is reactive with human (Homo sapiens), mouse (Mus), and canine (Canis) species. It exhibits approximately 30% cross-reactivity with recombinant canine IL-17 in direct ELISAs, making it suitable for studies involving these species.

Has this antibody been cited in any studies related to inflammatory diseases?

Yes, the IL-17/IL-17A antibody has been cited in multiple studies addressing inflammatory conditions, including its role in chronic inflammatory diseases such as interstitial cystitis and pancreatic cancer, as seen in and . These studies highlight the antibody's utility in understanding the immune response in these diseases.

Is there any evidence of this antibody being used in flow cytometry applications?

While specific references to flow cytometry applications for the IL-17/IL-17A antibody are not detailed in the provided information, it is listed among the applications for this antibody. Its use in flow cytometry has been implied in studies examining immune cell populations, such as those investigating T cell responses in various disease contexts.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease InflammationSmall MethodsMay 12, 2026Aditya Krishnakumar, Zhen Zhang, Courtney Vedelago, Timothy J. Liu, Yung‐Ching Kao, H. Peter Soyer, Mitchell S. Stark, Snehlata Kumari, Matt Trau, Alain WuethrichPBS. Subsequently, 10 µL PBS containing 5 µg mL−1 of antibodies against IL‐17A (R&D Systems, Cat no: AF317‐NA), IL‐22 (R&D Systems, Cat no: AF782), IL‐23p19 (R&D Systems, Cat no: AF1716), and TNF (R&D Systems,

(2) The role of IL-17 in orchestrating macrophage polarization via NF-κB, mTOR/HIF-1α and pyroptosis in diabetesScientific reportsFebruary 12, 2026Niu J, Liu G, He G, Zhang X, Bai H, Lu Y.

(3) Molecular characterization of chronic inflammatory diseases of the urinary bladder based on next-generation RNA sequencing and digital image analysisScientific ReportsOctober 31, 2025Yoshiyuki Akiyama, Jimpei Miyakawa, Masafumi Horie, Tetsuichi Saito, Tomonori Minagawa, Tetsuo Ushiku, Akiteru Goto, Haruki Kume, Yukio Homma, Yi Luo, et al.Bioscience, Tokyo, Japan), cytokeratin (1:100, clone AE1 + AE3, Dako, Glostrup, Denmark), IL-17 A (1:200, AF-317-NA, R&D Systems, Minneapolis, MN, USA), and mast cell tryptase (MCT; 1:4000, clone AA1, Dako, Glostrup,

(4) Lymphocyte loss and plasmacytosis are associated with IL-6- and TNF-producing cells in the spleens of fatal COVID-19 casesFrontiers in Cellular and Infection MicrobiologyOctober 23, 2025Bianca Ramos Mesquita, Lilian Verena da Silva Carvalho, Leonardo Cardoso Gomes Baqueiro, Reginaldo Brito, Luma Bahia Figueiredo Pinto, Erina Masayo Alves Hassegawa, Jonathan Luís Magalhães Fontes, Cláudio Pereira Figueira, Eraldo Salustiano de de Moura, Maria Brandão Tavares, et al.(Abcam-ab6671, 1/100), IFN-γ (Byorbit- orb10877, 1/50), IL-10 (Abcam- ab217941, 1/100) and IL-17 (R&D Systems- AF-317-NA, 1/20). After washing with 1x PBS, the slides were incubated with HRP Polymer Detection reagent (Vector

(5) Single-cell immunopathology of recurrent acute generalized exanthematous pustulosis associated with vancomycinJournal of Allergy and Clinical Immunology: GlobalJanuary 21, 2025Eric M. Mukherjee, Andrew Gibson, Matthew S. Krantz, Rama Gangula, Amy M. Palubinsky, Alan S. Boyd, Jeffrey P. Zwerner, Anna K. Dewan, Nontaya Nakkam, Katherine C. Konvinse, et al.were placed in a Protein Block (x0909, Dako) for 10 minutes. Slides were incubated with anti–IL-17 (AF-317-NA; R&D Systems, Minneapolis, Minn) for 1 hour at a 1:200 dilution, followed by biotinylated anti-goat

(6) Th9 and Th17 Cells in Human Ulcerative Colitis-Associated Dysplastic LesionsClinical Medicine Insights: OncologyDecember 7, 2024Guanglin Cui, Aping Yuan, Sveinung W Sørbye, Jon FlorholmenInc, Seattle, WA, USA) to label Th9 cells and goat anti-human IL-17A polyclonal antibody (Catalog No: AF-317-NA, clone Ile20Ala155, purchased from R&D System; Minneapolis, MN, USA) to label Th17 cells. After which

(7) Sex-dependent APOE4 neutrophil-microglia interactions drive cognitive impairment in Alzheimer's diseaseNature medicineOctober 1, 2024Rosenzweig N, Kleemann KL, Rust T, Carpenter M, Grucci M, Aronchik M, Brouwer N, Valenbreder I, Cooper-Hohn J, Iyer M, et al.

(8) Multiplex spatial omics reveals changes in immune-epithelial crosstalk during inflammation and dysplasia development in chronic IBD patientsiScienceAugust 16, 2024Matthijs J.D. Baars, Evelien Floor, Neeraj Sinha, José J.M. ter Linde, Stephanie van Dam, Mojtaba Amini, Isaäc J. Nijman, Joren R. ten Hove, Julia Drylewicz, G.Johan A. Offerhaus, et al.L8F6),Cell Signaling Technology,Cat# CST6956BF; RRID: AB_2797704 IL-17a (polyclonal),R&D Systems,Cat# AF-317-NA; RRID: AB_354463 Ki-67 (clone B56),BD Biosciences,Cat# 550609; RRID: AB_393778 Granzyme B (clone D6E9W),Cell

(9) IL-17a promotes hepatocellular carcinoma by increasing FAP expression in hepatic stellate cells via activation of the STAT3 signaling pathwayCell Death DiscoveryMay 13, 2024Dapeng Sun, Wen Li, Dongyang Ding, Kunjiang Tan, Wenbin Ding, Zongyan Wang, Siyuan Fu, Guojun Hou, Wei-ping Zhou, Fangming GuAB_2837829), anti-GAPDH (Affinity Biosciences Cat# T0004, RRID: AB_2833041), anti-IL17a (R&D Systems, #AF-317-NA), anti-Stat3 (79D7) (CST, #4904), anti-phospho-Stat3 (Tyr705) (D3A7) (CST, #9145), anti-phospho-Stat3 (Ser727

(10) The Immune Microenvironment Landscape of Pituitary NeuroEndocrine Tumors, a Transcriptomic ApproachGenes (Basel)April 24, 2024Sandra Vela-Patiño, Ma. Isabel Salazar, Keiko Taniguchi-Ponciano, Eduardo Vadillo, Erick Gomez-Apo, Aurea Escobar-España, Vadim Perez-Koldenkova, Laura Bonifaz, Cristina Aguilar-Flores, Daniel Marrero-Rodríguez, et al.(R&D systems, Minneapolis, MN, USA, #cat AF2408), and anti-IL-17 dilution 1:100 (R&D systems, #cat AF-317-NA). Incubation of secondary antibodies anti-goat AF594, anti-rabbit AF647, anti-rabbit 594, and anti-mouse

(11) NFκB and NLRP3/NLRC4 inflammasomes regulate differentiation, activation and functional properties of monocytes in response to distinct SARS-CoV-2 proteinsNature communicationsMarch 7, 2024Tsukalov I, Sánchez-Cerrillo I, Rajas O, Avalos E, Iturricastillo G, Esparcia L, Buzón MJ, Genescà M, Scagnetti C, Popova O, et al.

(12) Identification of immunological patterns characterizing immune-related psoriasis reactions in oncological patients in therapy with anti-PD-1 checkpoint inhibitorsFront ImmunolMarch 1, 2024Martina Morelli, Maria Luigia Carbone, Giovanni Luca Scaglione, Claudia Scarponi, Valentina Di Francesco, Sabatino Pallotta, Federica De Galitiis, Siavash Rahimi, Stefania Madonna, Cristina Maria Failla, et al.France, 1:30 dilution), anti-CD15 (#347420, BD Biosciences, Milan, Italy, 1:30 dilution), anti-IL-17A (#AF-317-NA, R&D Systems, Abingdon, UK, 1:30 dilution) and anti-ADAMTSL5 (#NBP1-93438, Novus Biologicals, Centennial,

(13) IL-23 induces CLEC5A+ IL-17A+ neutrophils and elicit skin inflammation associated with psoriatic arthritisJournal of AutoimmunityFebruary 1, 2024Hiroki Furuya, Cuong Thach Nguyen, Trevor Chan, Alina I. Marusina, Alexander A. Merleev, Maria de la Luz Garcia-Hernandez, Shie-Liang Hsieh, George C. Tsokos, Christopher T. Ritchlin, Ilias Tagkopoulos, et al.system (Olympus VS120). For immunofluorescence, sections were blocked and stained with goat anti-IL-17 (AF-317-NA, R&D systems), mouse anti-CD66b (NB100–77808SS. Novus Biologicals), and rabbit anti-MRP8 (SA100A8),

(14) Th1/17 polarization and potential treatment by an anti-interferon-γ DNA aptamer in Hunner-type interstitial cystitisiScienceNovember 17, 2023Yoshiyuki Akiyama, Kaori Harada, Jimpei Miyakawa, Karl J. Kreder, Michael A. O’Donnell, Maeda Daichi, Hiroto Katoh, Miyuki Hori, Kensuke Owari, Kazunobu Futami, et al.clone 236A/E7, Abcam, Cambridge, UK), IFN-γ (1:200, ab218890, Abcam, Cambridge, UK), IL-17A (1:200, AF-317-NA, R&D Systems, Minneapolis, MN, USA), mast cell tryptase (MCT) (1:4000, clone AA1, Dako, Glostrup, Denmark),

(15) Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitisiScienceOctober 20, 2023van Eijs MJM, Ter Linde JJM, Baars MJD, Amini M, Laclé MM, Brand EC, Delemarre EM, Drylewicz J, Nierkens S, Verheijden RJ, et al.AB_2894896 CD56 (clone NCAM1/784),Abcam,Cat# ab216010; RRID: AB_2909431 IL-17A (polyclonal),R&D Systems,Cat# AF-317-NA; RRID: AB_354463 Ki67 (clone B56),BD Biosciences,Cat# 556003; RRID: AB_396287 Granzyme B (clone D6E9W),Cell

(16) Clinicopathologic Characteristics of PANDAS in a Young Adult: A Case ReportDevelopmental NeuroscienceSeptember 12, 2023Lakshmi Shree Kulumani Mahadevan, Melissa Murphy, Marina Selenica, Elizabeth Latimer, Brent T. Harris1:80), RORgammaT (Biocare, catalog no.: ACI3208A, dilution: 1:100), IL17A (R&D Systems, catalog no.: AF-317-NA, dilution: 1:75), and Foxp-3 (Abcam, catalog no.: ab20034, dilution: 1:50) and photographed using spectral

(17) IL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin BiopsiesJID InnovJuly 1, 2023Chen JS, Murphy MJ, Singh K, Wang A, Chow RD, Kim SR, Cohen JM, Ko CJ, Damsky W.IL-17 (Ab number #1: Abcam, Cat number #ab79056, Cambridge, UK) (Ab number #2: R&D Systems, Cat number #AF-317-NA; Minneapolis, MN), IFN-γ (Ab number #1: Abcam, Cat number #ab25101) (Ab number #2: R&D Systems, Cat

(18) Canakinumab leads to rapid reduction of neutrophilic inflammation and long-lasting response in Schnitzler syndromeFrontiers in medicineJanuary 1, 2023Bossart S, Seyed Jafari SM, Heidemeyer K, Yan K, Feldmeyer L, Borradori L, Yawalkar N.(clone NP57, DakoCytomation), IL-1β (clone 11E5; Abcam), IL-8 (clone 807; Abcam), and IL-17 (polyclonal, AF-317-NA, R&D Systems, Minneapolis, MN, United States). Irrelevant IgG subclass-matched antibodies were used

(19) Clinical significance of CD155 expression and correlation with cellular components of tumor microenvironment in gastric adenocarcinomaFrontiers in immunologyJanuary 1, 2023Liu X, Xu C, Guo T, Zhan S, Quan Q, Li M, Wang Z, Zhang X, Guo L, Cao L.anti-CD4 (UMAB64, ZSGB-BIO), anti-CD8 (EP334, ZSGB-BIO), anti-Foxp3 (1054C, R&D Systems), anti-IL-17 (AF-317-NA, R&D systems), anti-IFN-γ (sc-74108, Santa Cruz Biotechnology), anti-CD19 (EPl69, ZSGB-BIO), anti-CD11c

(20) Insight into immune profile associated with vitiligo onset and anti-tumoral response in melanoma patients receiving anti-PD-1 immunotherapyFrontiers in immunologyJanuary 1, 2023Carbone ML, Capone A, Guercio M, Reddel S, Silvestris DA, Lulli D, Ramondino C, Peluso D, Quintarelli C, Volpe E, et al.LSBio-B7396-50; LS Bio, Seattle, WA; 1:5); mouse monoclonal antibody anti-interleukin (IL)-17A (cod. AF-317-NA, R&D Systems, Minneapolis, MSP; 1:30); monoclonal antibody anti-CD56 (cod. NCL-CD56, Novocastra Scytek,

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Human IL-17/IL-17A Antibody